GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGK13 978 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human germline pseudogene for the leader peptide and variable
region of a kappa immunoglobulin (subgroup V kappa II)
ACCESSION Z00012 X00904
KEYWORDS immunoglobulin; immunoglobulin light chain; kappa-immunoglobulin;
pseudogene; signal peptide.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (sites)
AUTHORS Zachau,H.G.
JOURNAL Unpublished (1984)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 978)
AUTHORS Pech,M., Jaenichen,H.-R., Pohlenz,H.-D., Neumaier,P.S.,
Klobeck,H.-G. and Zachau,H.G.
TITLE Organization and evolution of a gene cluster for human
immunoglobulin variable regions of the kappa type
JOURNAL J. Mol. Biol. 176, 189-204 (1984)
STANDARD full automatic
COMMENT The sequence is designated Vc in ref. [1]; gaps are not included.
From EMBL entry HSIGK13; dated 13-JUN-1985.
FEATURES Location/Qualifiers
intron <1..199
/note="first intron"
misc_feature 200..202
/note="stop codon (pseudogene)"
CDS 200..510
/note="second exon (rest of L and variable region) (200 is
1st base in codon)"
/codon_start=200
misc_feature 449..449
/note="frameshift mutation (pseudogene)"
misc_feature 513..519
/note="heptanucleotide box, not canonical"
BASE COUNT 279 a 231 c 210 g 258 t
ORIGIN
1 gatctgtgag agtgaggaag attccagaag gagcaaggat atgtaaatat tggttctttt
61 taaatctgta tgttttggga ggattaatca aaatcacaca cacaaaaata attgagcaaa
121 acataaataa cagacagaaa atgattaaaa tgactcacaa tgtttgcaca taaccttgca
181 cttctctctc attatttcat gatccaatgg agatgctgtg atgacccaac ctccactctc
241 cctgcccatc acccctggag agccagcctc catctcctgc aggtctagtc acagccccct
301 tcacagtaat ggatacatct atttcaactg gtacctacag aagacaggcc agcctccttg
361 gctcccaatc tatttggttt ccaatcacga ccctggagtc ccagacaggt tcagtggcag
421 tgggttgggg acagatttca tgctgaaatc aggaggatgg atgctgagga tgttggggtt
481 tattgctgcc agcaaagtac acattatcct cccacaatgg tatagtcttg aacacaaacc
541 cccccacttg ctgtggccta gctgcccaga tgtgctgttt ctgtggagag caggcactgt
601 ggattctctt agatgcctaa agaccaagat gttggagaac tcagaggact tggtgcagct
661 gagggctcat gaccataaat ttcttggcta cacctcaggc accacatttt aaggtcccgt
721 cagctgcagc agcctttgca tgacagagtc tgcagtatgg aggaggtcca cgtgccctct
781 gagcaatgag acacaagaga agagaaggct caatgagagc tcattctaat cctctcttcc
841 ttccctatgt tcattcatca actaaattca ttctgcataa caggcaccta attgagactg
901 attactggca acacaaaact aacacattct tttgatttgg tttagcagtt accagagtag
961 atgtagattg ataagatc
//GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGK5 1157 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human germline pseudogene for the leader peptide and variable
region of a kappa immunoglobulin (subgroup V kappa I)
ACCESSION Z00003 X00747
KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; pseudogene;
variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 1157)
AUTHORS Jaenichen,H.R.
JOURNAL Unpublished (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1157)
AUTHORS Jaenichen,H.R., Pech,M., Lindenmaier,W., Wildgruber,N. and
Zachau,H.G.
TITLE Composite human V kappa genes and a model of their evolution
JOURNAL Nucleic Acids Res. 12, 5249-5263 (1984)
STANDARD full automatic
COMMENT The sequence is designated V14 in refs. [1] and [2]; the start and
stop positions of the leader peptide were defined according to
sequence comparisons; several mutations render the leader gene
segment inactive; the variable region gene segment is inactivated
by a stop codon at the amino acid position 71.
From EMBL entry HSIGK5; dated 13-JUN-1985.
FEATURES Location/Qualifiers
misc_feature 2..16
/note="put. regulatory element pd"
misc_feature 34..43
/note="put. regulatory element dc"
promoter 77..82
/note="TATA-box"
CDS 130..184
/note="first exon (leader peptide, inactive) (184 is 1st
base in codon)"
/codon_start=130
intron 185..308
/note="first intron"
CDS 309..604
/note="second exon (rest of L and variable region) (309 is
2nd base in codon)"
/codon_start=309
misc_feature 607..613
/note="heptanucleotide box"
BASE COUNT 289 a 292 c 289 g 287 t
ORIGIN
1 ctgcagctgt gctcagcctg ccccatcccc tgctgatttg cctgttccta gagcacagcc
61 ccctgccctg aagacttttt ataggctggt cacacccggt gcaggagtca gccccagtca
121 ggacacagca cggatgcaag gtcccccact cagctcctgg ggctcctggt gctctggctg
181 ccaggtaagg aaggagaaca ctaggattat actcggtcag tgtgctcagt actgtctgga
241 acctcaggga agtcctctga taacatgatt aattgcaaga atatttgttt ttatgtttcc
301 aactgcaggt gccagatgtg acatccagat gacccagtct ccatcctccc tgtctgcatc
361 tgtaggaggc agagtcacca tcacttgccg ggcgagtcag ggcattagca ataatttaaa
421 ttggtatcag cagaaaccag ggaaaactcc taagcccctg atctatgctg catccagtct
481 gcaaagtggg attccctctc agttcagtga cagtggatct gggacagatt agactctcac
541 catcagcagc ctgcagcctg aagattttgc aacttattac tgtcaacaga gttacagtac
601 ccctcccaca gtgttgcaag tcataatata aaccccaagg aagcagatgt gtgaggctgg
661 gctgccccaa tgctccttct ggtgcctcta tctgctgagg gaagttctca aactcagtca
721 ggcttggaaa gtcattggga gattttccta gaggaggtca gggaggttcc tctgaaccct
781 aagcctcttt cgccctcatc cccagcagac aagatgtgac aatgcctgtc ctgactgaat
841 aaagaagaga gataagtcca gctgaggagt ctgtgttatg ggataatcgg aatttgtaca
901 gcaaaagaga agctattctc agtatttcaa ggagaaatta ttcaagttga ataaattaga
961 gtctaaacca cagtctttcc gaagcctatg gagtgttatt catgaagcag gtactagaca
1021 caggggattc tcaggtgcta cttcagaagc cagggtgcac ctgcccctgg tggtatgtgc
1081 tgaacaccat gtgatgatcc tcagtcctgt ctgggaagcc cagggctggg ggtgctgatg
1141 ctctcagctg cctgcag
//GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGK6 1247 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human germline pseudogene for the leader peptide and variable
region of a kappa immunoglobulin (subgroup V kappa I)
ACCESSION Z00004 X00748
KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; pseudogene;
variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 1247)
AUTHORS Jaenichen,H.R.
JOURNAL Unpublished (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1247)
AUTHORS Jaenichen,H.R., Pech,M., Lindenmaier,W., Wildgruber,N. and
Zachau,H.G.
TITLE Composite human V kappa genes and a model of their evolution
JOURNAL Nucleic Acids Res. 12, 5249-5263 (1984)
STANDARD full automatic
COMMENT The sequence is designated V52 in refs. [1] and [2]; the variable
region gene segment is inactivated by a frameshift mutation
(insertion of a G) at the position 886)
From EMBL entry HSIGK6; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 4..47
/note="simple sequence region"
unsure 69..69
/note="A or G or T"
unsure 77..77
/note="A or C or T"
unsure 89..89
/note="A or C"
unsure 211..211
/note="A or G or T"
unsure 349..349
/note="A or T"
misc_feature 416..425
/note="defective (due to a deletion) put. regulatory
element pd"
misc_feature 427..436
/note="put. regulatory element dc"
promoter 470..477
/note="TATA-box"
CDS 525..579
/note="first exon (leader peptide) (579 is 1st base in
codon)"
/codon_start=525
intron 580..710
/note="first intron"
CDS 711..1000
/note="second exon (rest of L and variable region) (711 is
2nd base in codon)"
/codon_start=711
misc_feature 886..886
/note="frameshift mutation (insertion of a G)"
misc_feature 1003..1009
/note="heptanucleotide box, not canonical"
BASE COUNT 335 a 299 c 270 g 338 t 5 others
ORIGIN
1 aaatctatct atctatctat ctatctatct atctatctat ctatctatca ttcatgatta
61 taccttaang catccantgt tggtagcant gacaatttac agcactggtg tttccaggga
121 attggaccaa aaaggaagtc tctctgacct taatagtact catctgtatc aaatgcagga
181 aacttctaaa atttcttgag tttctagaga ngtttttccc tagcagacct tatcataaat
241 agaaagctag caagagaagc atgtcatgaa acatgaagag agcaaaagaa cactccacac
301 ataggacagt aggctgattc tttcctgtag cctgcaggga gaaacacang ctctgcagac
361 tttggacacc tgggaggcac tgggcctgtg cagtgttatt gagataagtc atctttgcag
421 ctgtgcagat ttgcatgtcc cacagagcaa cgcctactgc cctgaacatt tatcaatagg
481 ctggtgacat cctgtgcaga agtctctctc agtccagaca cagcatggac atgagggccc
541 ccgctcagct cctgggactc ctgctgctct ggctcccagg taaggaggga aacaacaaaa
601 attttattca gccagtgtag ccattaatgc ctggcacttc aggaaattct tcttagaaca
661 ttactaatca tgtggatatg tgtttttatg ttcctaatat cagataccag atgtgacatc
721 cagatgaccc agtctccatc ctccctgtct gcatctgtag gagacagagt caccatcact
781 tgccgggcga gtcagggcat tagcaattat ttagcctggt atcagcagaa accagggaaa
841 gttcctaagc tcctgatcta tgctgcatcc gctttgcaat cagggggtcc catctcggtt
901 cagtggcagt ggatctggga cagatttcac tctcaccatc agcagcctgc agcctgaaga
961 tgttgcaact tattactgtc aaaagtataa cagtgcccct cccactgtga tacaagcccg
1021 aacataaacc atggagggaa gtagatgtgt gagtctgggc tgccccagct gctcctcctg
1081 gtgccgccgt ctgctgacag cagttctcag atgcagccaa ggtttgaagc tccctgggaa
1141 gttttggtag aaggggtcag ggaggcacat ttacattcta tctctcttta tcctcagctc
1201 catcagctga tatgcaagta tctctcctga ttattattaa taaagga
//GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGK7 661 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human germline pseudogene for the leader peptide and variable
region of a kappa immunoglobulin (subgroup V kappa I)
ACCESSION Z00005 X00749
KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; pseudogene;
variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 661)
AUTHORS Jaenichen,H.R.
JOURNAL Unpublished (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 661)
AUTHORS Jaenichen,H.R., Pech,M., Lindenmaier,W., Wildgruber,N. and
Zachau,H.G.
TITLE Composite human V kappa genes and a model of their evolution
JOURNAL Nucleic Acids Res. 12, 5249-5263 (1984)
STANDARD full automatic
COMMENT The sequence is designated V55 in refs. [1] and [2]; the variable
region gene segment is inactivated by several frameshift mutations
From EMBL entry HSIGK7; dated 13-JUN-1985.
FEATURES Location/Qualifiers
CDS <1..27
/note="first exon (leader peptide) (1 is 2nd base in
codon) (27 is 1st base in codon)"
/codon_start=1
intron 28..151
/note="first intron"
misc_feature 28..29
/note="not canonical donor splice site"
CDS 152..447
/note="second exon (rest of L and variable region) (152 is
2nd base in codon)"
/codon_start=152
misc_feature 450..456
/note="heptanucleotide box"
BASE COUNT 162 a 167 c 152 g 180 t
ORIGIN
1 cgggacttct gccatcctgg tttccagtta agaaaagaaa ttactagcct cttactcagc
61 cagtgtgcta ggtactgcct ggccattcag ggaagtcttc ttataacatg attaattgtg
121 tggatatttg tttttctgtt tgcaatttca gggaccagaa gtgacatcca gtcacccagt
181 ctccatccac actgtctgca tctgtaagga gacagcgtca ccatcacttg ccgggcgagt
241 cagagcattg gtagtaattt agactggtat cagcagaaac cagggaaggc ccctaagggc
301 ctgatctatg ctgcatccag tttgcaatct ggggctcctt cgcggttcgg tggcagtgga
361 tctgggacag attttactct caccatcaga atcctgcagc ctaaagatgt tgcaagttat
421 tactgtcaac agtataaaaa ttaccctatc acagtgttac aaaccataac aaaccccccc
481 caggaaagca gacatgtgac gctgggctgc cccacctgct cttctttgtg cagccatctg
541 gtgacaacac ttctcagact cagcctgagt tttgagggtt attgggagat tacggaagag
601 ggggccatga aggtgtctct ccaccctaag tctctttcct ctcatggcaa tgtctcttcg
661 a
//