GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGKVII 490 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human rearranged DNA for kappa-immunoglobulin leader peptide and
variable region
ACCESSION X03960
KEYWORDS immunoglobulin; kappa-immunoglobulin; rearranged DNA.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 447 to 490)
AUTHORS Hieter,P.A., Maizel,J.V.Jr.. and Leder,P.
TITLE Evolution of human immunoglobulin kappa J region genes
JOURNAL J. Biol. Chem. 257, 1516-1522 (1982)
STANDARD full automatic
REFERENCE 2 (bases 1 to 311)
AUTHORS Klobeck,H.G., Solomon,A. and Zachau,H.G.
TITLE Contribution of human V kappa II germ-line genes to light-chain
diversity
JOURNAL Nature 309, 73-76 (1984)
STANDARD full automatic
REFERENCE 3 (bases 1 to 490)
AUTHORS Klobeck,H.G.
JOURNAL Unpublished (1986)
STANDARD full automatic
COMMENT The variable region gene segment is recombined with the J4 gene
segment; the same V gene segment is recombined with J1 in cell line
GM607
From EMBL entry HSIGKVII; dated 18-NOV-1986.
FEATURES Location/Qualifiers
CDS 135..485
/note="leader peptide and variable region fragment (exon
2)"
/codon_start=135
conflict 235..235
/note="c at pos. 91 in [3]"
misc_feature 446..446
/note="frameshift mutation, aberrant joining"
intron 486..>490
/note="intron II"
BASE COUNT 130 a 104 c 114 g 142 t
ORIGIN
1 gaattcattt tagaatgtgt atttttatgg cataaatcac tattttttaa aattaagttt
61 aaataaatga cataagataa attatgaaaa ttgctcatta ggtttgtaca taactttgca
121 attcattatt tcaggatcca gtggggatat tgtgatgact cagtctccac tctccctgcc
181 cgtcacccct ggagagccgg cctccatctc ctgcaggtct agtcagagcc tcctgcatag
241 taatggatac aactatttgg attggtacct gcagaagcca gggcagtctc cacagctcct
301 gatctatttg ggttctaatc gggcctccgg ggtccctgac aggttcagtg gcagtggatc
361 aggcacagat tttacactga aaatcagcag agtggaggct gaggatgttg gggtttatta
421 ctgcatgcaa gctctacaaa ctcccgctca ctttcggcgg agggaccaag gtggagatca
481 aacgtaagtg
//