GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGU266 8381 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human U266 rearranged DNA for lambda-immunoglobulin light chain
ACCESSION X51754
KEYWORDS immunoglobulin; immunoglobulin light chain;
immunoglobulin superfamily; joining region, constant region;
pseudogene; rearranged DNA; variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 8381)
AUTHORS Vasicek,T.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Vasicek,T.J. and Leder,P.
TITLE Structure and expression of the human immunoglobulin lambda genes
JOURNAL J. Exp. Med. 172, 609-620 (1990)
STANDARD full staff_review
COMMENT *source: strain=cell line=U266; cell type=myeloma; clone=pU9.0;
**map: chromosome=22;
From EMBL entry HSIGU266; dated 03-JAN-1991.
FEATURES Location/Qualifiers
misc_feature 1469..1476
/note="octamer repeat"
TATA_signal 1502..1506
misc_feature 1532..1616
/note="pseudo exon 1 (leader exon)"
misc_feature 1571..1573
/note="atg start"
misc_feature 1617..1902
/note="intron within pseudo-V region"
misc_feature 1903..2197
/note="pseudo-V exon 2"
misc_feature 2198..2204
/note="heptamer recombination signal"
misc_feature 2228..2236
/note="nonamer recombination signal"
misc_feature 3122..3129
/note="octamer repeat"
misc_feature 5154..5162
/note="octamer repeat"
TATA_signal 5193..5197
exon 5220..5308
/note="exon 1 (leader exon)"
CDS 5263..5308
/note="lambda-immunoglobulin light chain V-region (V266)
(AA 1-15) (5263 is 1st base in codon)"
/codon_start=5263
intron 5309..5423
/note="intron I"
exon 5424..5765
/note="exon 2"
CDS 5424..5765
/note="lambda-immunoglobulin light chain V-J region (AA
16-129) (5424 is 2nd base in codon) (5765 is 1st base in
codon)"
/codon_start=5424
intron 5766..7057
/note="intron II"
exon 7058..7496
/note="exon 3"
CDS 7058..7374
/note="lambda-immunoglobulin light chain C-region (AA
130-235) (7058 is 2nd base in codon)"
/codon_start=7058
misc_feature 7480..7485
/note="put. polyA signal"
misc_feature 7491..7596
/note="put. polyA signal"
BASE COUNT 1976 a 2283 c 2175 g 1947 t
ORIGIN
1 aggcatcagg gacctgagca gtaggttctg ttcctatgcc tggacccagg aggcccatga
61 gcctcagcag tcagtatgga gcagccacca ggggggcagc aaagagtcac ctgggaaagc
121 cacctcggga tggggtggac ctgggcatca gggagatgct gacatgcagg gagggtcagt
181 gaccacgacc tgacacctgg agaaaatagg tccttctttt tctagagtgt tgtccttgac
241 actgatgtaa aaattctagc atcaaatttt atcttcagct tttgtagatg ctcctatttc
301 cctgacaaag gtccaggcac ttgacttccc cacacatcct ctcctcacac acccagttgt
361 gctaaaatgc tgtccctgtc acgcacagtg tccttcctga ggtgacacgt gtccccctcc
421 ctgccctttg ctctgggagc ccctgtcctg tagacacagc agcttcttct tccctggctg
481 ctccccatct ccactgtccc tgggctttcc catctgtctc ctgggaaagc tgccaaatca
541 caggtctcaa tttccttgtg aggcacaaaa tagttgctca ttaaccttct gttgacttaa
601 ttaagttttt aattccttga tttgttttcc ccagcaactg aagacaactt agtcataata
661 aacattcatt tccaggggtc ttgcagtttg ctgacccctc tcattctctc atggagcaaa
721 cattttcttg acctccttat ctgtcattgg tcctcacagc accctgggga cactgaggca
781 cagcatgtac gacctgccct ggagctcaca gccaacgagg aaacttaccg catcccatgg
841 atcttccaac actccatgtg cacttggcaa ccaagaaatt ttattcttct ctagcactga
901 ggagctgtgc cctggggtgg ggtctgtacc tatccaaggc acatgctgtc acctgtagag
961 tgtggataga gggtccttgc attaggttct ggaaagtgtc ttcaaaaacg gatttaggcc
1021 tacacccaaa atctcctctc tggcacaaat tattcatgat caggatacat ctagaggcct
1081 agaccaaaat tcaccacctc cctcctgagc cagaatggaa tggaggaggg gactggggac
1141 cccagataat ttccccatca ccactgtgac tctatggcca cctcctgcaa cagttaggga
1201 taatcacata ggaagggctt catgtatcct aggaaaaaca cccagagtca cttatgagct
1261 ctaccatgac caagtgagcc caggagtcgg gttgagcaca gcgtggtgtg gagatatctg
1321 cagagaccgt ggaaccagtt tgtaaaggag atcctgtagg ctgagggtga gagtgcaagg
1381 ccactcctcc ccctctctgc atcctttggg gctaagccct tctctggaaa ccacaaagct
1441 cctccagtag cagcccttga ctctgctgat ttgcatcaca ggctgctctc ttcagcaagg
1501 ggataagaga gggctggaag gaacctgccc agcctgggcc tcaggaagca gcatcggggg
1561 tgccgcagcc atggcctgga ccgctctcct tctgagcctc cttgctcact ttacaggtgc
1621 tgcccccagt gtcccagcca cctacccagc tccaaggctc tgggtccagc ctggcctgac
1681 agtgatctca gcagggccct gcctgtggtg tgcaggatgc tcatgatcct gctgcagggg
1741 gaggggctgc tggaggtgaa atccccccac actgttcttc tgtgctcatg gtcccctgag
1801 gacacttcta ttcctgaaac tcaggccagg caggtgggaa ggcattgttg ggttgagcct
1861 ctcagtttca agtctattct attctctccc cttttcttgc aggttctgtg gcctcctatg
1921 agctgactca gccactctca gtgtcagtgg ccctgggaca ggcggccagg attacctgtg
1981 ggggaaacaa ccttggatat aaaatgtgca ctggtaccag cagaagccag gccaggcccc
2041 tgtgctggtc atctataggg ataacaaccg gccctctggg atccctgagc gattctctgg
2101 ctccaactcg gggaacacgg ccaccctgac catcagcaga gcccaagccg gggatgaggc
2161 tgactattac tgtcaggtgt gggacagcag cactgcacac agtaacacag gcagatgaag
2221 aagtgagaca caaacccttt cgccatctat gtcaccctct ttctctaatt caaggaggac
2281 tatgaacaaa gccttacgca gatctggtta aattctcttt aatctctgcc cccagcctgt
2341 ccctccctcc agtccttcag gcggggtctg cagaaagtgc atcagtagtt catttagggt
2401 gatgtgatca ggatggactg gtgttttggt gactcagctg tgacttggga aaatagggcc
2461 tgcatggaag atcagactga ggtagacatc cacagttgtc tctggatttc cataggtggt
2521 gacaggccag tccttgcccc aaatgtccta gatcaggggc cagaactgcc cagtctcagg
2581 cccctgtggt agcctgtcat tctttagaga acaccaagga agtagacact gtgggatctg
2641 cccagatccc ctgttctgag cttacccact aagcactact gggagcttct ggcaggtcag
2701 agctgtgtcc tcactgtaaa gtgctgttcg gtgcagaaaa cagactcctc caggtctatg
2761 ccctttctca gtgaggttag atttcatgac tgcctgaggc cgagttctcg actctgcctc
2821 aatataggaa gacctgaaaa tcctcccagc tccacagctc tctgtaggaa agacttgaga
2881 cctcaatgag ggtcagggtc tcctgcccag ggttgcctcc ctcacctcct ttcacatcct
2941 ctgtgcatgc aattcctcat ctcagagtct gcttccaggg aacccaatta agatcaccag
3001 ccatccccaa catgggccat gtgggacttc aggaggccac tgtatgctgg aaagcacctg
3061 tttattccta aattagcact gtgagtatcc acacgcccaa tgagatctga gtgtttcttg
3121 catttgcatg gaatgagaag ggagcctgac tcgccaggtg ctctggggtc taagggagag
3181 ttggagtcag atctccctgc aggaaacctg gggcaggagg agcagcctta ccccatgcag
3241 ggaccacaga tgcacccaca gggtgagctt aagtatggac actgcctacc tccaccctcc
3301 acatcctgtg taaaaaatca ttctttccac ccctcctcca gcctatcaaa gttgacactt
3361 aaaaagccac cacaacccac ccttgtcaac aggaaaccca ttcacatctc catcagccac
3421 acaatctcca cataaagatt cttcaaaaaa taataacaat agccatgcaa agtatatgat
3481 atatatcata aataattgta cttagattat atatgaaaat gatactcttt ttgatatgct
3541 aggtaaataa aatacattat ttaaactcat tgacttcttc tctttgtatt tataacatga
3601 tgactagaca gtgggattcc catgaggctc acatcacatt gtgattgaac aaggctggtc
3661 tgcagggctt caggttacag gggctgtgga gtgacctggt gcaggaagcc ccgtctccat
3721 ccataggaca tcagtgtgaa cccagggaga ggcgctggta taagagagat gagaacacag
3781 gtgtgaacca ctgcttgtgc aggggactga gccttcactt tgagccaaat aagttttctt
3841 tcccaagttc gatcctgaac aagagggctt tctggacttc tttttgtttt caggtttgtg
3901 ccccttcagg tttggggctg tctggagtcc cagccagggg acagtgagga aatccagcag
3961 tctcacactg actctgttgt atttcatctg ctggggccat cccaaactgc tgcgtaccat
4021 ttcctttcct ggtgctcaaa tagctgctcc atgcacttgt catggtttta tagctcaatc
4081 tagatgggaa gacaggatag tgtatgttac aatatttaaa tagaacctga atcccttaat
4141 agttatttta atatggaaca ttaaaagagt tgcatcatag gcaatgggga aagatgaaga
4201 aagattaaac gcatttggag gaaaaactaa aaatgtcttt attgaatgtc tgtgtgtctt
4261 cagtgtcgaa gggttgagag aggtcagctt taggttgtcc tgaggatgtg ctcagatggg
4321 ggaaagtaag aaagccggaa acgtgcatct ctgtgacctg acacataaat cacagtaaaa
4381 tggaagtaaa tctttcaata aacctttcaa gataacatca ctctggtgca catagaaagg
4441 cctgttagca aactttccct tttccctgct ggctcatatc cccatggatc acctgtatgc
4501 agaacggact ctgtggtctc catttatcag gctgagaacc agaggctggg aaggacaagt
4561 cacctgctca tgagtgggag gggcaggatt gggagcagcg gactcaggct caggactgtg
4621 gccctggttg gtgctccttg ttcccttcac agggtcccac ccacatgtgc ccgtgtggat
4681 gtgggtgcac tgcctggggt ggctgatgtg ctccagggat ttgatggtgc agatgcttca
4741 ggacaaagca tgggagtggg gatgggacga ggctgccgct gggcagacca gggaccaacg
4801 ccacccagca gaggcttcca gtggggttgg gcagccccat ttcctgagaa tagagagaac
4861 ctggcaatga gtgccagaaa gaggttactt cgaggtgtgc ccaggcctgg agggtcacag
4921 agacacaggc accacacagc agagacactg agggccaaga gctcactcag gtgaggggct
4981 tcagcagatt tttctctcct gagcaaatca cgtgcaaaga aatcaacttc ctgtcatcag
5041 aatagacaat cagggacttt agtctccttg ctgagccccg ctgtcaaggg aagcagaagt
5101 ctctaagccc aggcccaagt gagggtgggg tgagaagagg agctcaggat gcagatttgc
5161 atggaggtcc cgcccttctc tgaggcagag gggataagac agggctgggg gcaggcccag
5221 tgctggggtc tcaggaggca gcgctctcag gacgtcacca ccatggcctg ggctctgctc
5281 atcctcaccc tcctcactca gggcacaggt gatgcctcca gggaaggggc cacagggacc
5341 tctgggctga tccttggtct cctgctcctc aggctcacct gggcccagta gtgactcact
5401 agactgtctt tctccctttc cagggtcctg ggcccagtct gccctgactc agcctccctc
5461 cgcgtccggg tctcttggac agtcagtcac cttctcctgc agtggaacca gcagtgacat
5521 tggtaattat aattatgtct cctggtaccg acaacaccca ggcaaagccc ccaaactcat
5581 gatctatgag gtcactaagc ggccctcagg ggtccctaac cgcttctctg gctccaagtc
5641 tggcaacacg gcctccctga ccgtctctgg gctccaggct gaggatgagg ctgattatta
5701 ctgcagctca tacgcaggca gtaatagttt gattttcggc ggagggacca ggctgaccgt
5761 cctaggtaag tctcttctcc cctctccttc cccactcttg ggacattttc tgctggtttt
5821 ccttggttct gtatcttgtc tcaatttgtg gtcagccttc tccctgcatc ccaggcctga
5881 gaaaggacct ctgccctccc tgttcagacc ctaccaacca cttcacctct gaccgcaggg
5941 gcgggggaca agatagagtg accgactgag cctcgtctgt ctgactgtct gcctctctgt
6001 ttgtctgtct gtctctctgt ttgtctctct gtctgtctga caggcgcagg ctgggtctcg
6061 aagccttgtt ctgttctggc ctccgcagtc tgggttcttg tcggaacagc tttgcccttg
6121 ggttacctgg gttccatctc ctggggagtt gggaacaagg ggtctgaggg aggctcctcc
6181 tgggagactt tagaaggacc cagatgccct cggggctgat gctcgggaat cacagacctg
6241 ggacccagag ccaggatcca gacccagaat gaggtgggag gtggaggggc tgccctgggc
6301 agtctggggg ctgccaggga ctgagccctg agccagcccg agactcagga gaccccgtca
6361 ggacggagaa gggagaagca gactctggac accagaaagg caggggaagg gtcacaaaag
6421 gagtggatgt gacggaaggg cgggctcctg ggtctcttca gaacatatcc cctgtgtcca
6481 gggggatcag aggggcagag tccactgcgt gaaagcccca ctgctacgac caggtagccg
6541 ggacgtgggg tggatgccag aaaagactcc atggaataag agagagtcca ggacagcagg
6601 caggctctcc gatcccccca ggcccttgcc ccaaacacgg gctccagaac acacatttgc
6661 tggaacagcc tgagggacca aaaggcccca gtatcccaca gagctgagga gccaggccag
6721 aaaagtaacc ccagagttcg ctgtgcagga gagacacaga gctctctttt tctgtcagga
6781 tggccggagg gggcagggtc agggcgctga gggtcagatg tcggtgttgg gaaggccccg
6841 agagatctcc ggacaggagg tcaggtgtct aaggtaaaac acctccccgt gcagatcagg
6901 acatagtgga aaacaccctg acccctctgc ctggcataga ccttcagaca cagagcccct
6961 gaacaagggc accccaacac ctcatcatat actgaggtca ggggctcccc aggtggacac
7021 caggactctg accccctgcc cctcatccac cccgcaggtc agcccaaggc tgccccctcg
7081 gtcactctgt tcccgccctc ctctgaggag cttcaagcca acaaggccac actggtgtgt
7141 ctcataagtg acttctaccc gggagccgtg acagtggcct ggaaggcaga tagcagcccc
7201 gtcaaggcgg gagtggagac caccacaccc tccaaacaaa gcaacaacaa gtacgcggcc
7261 agcagctatc tgagcctgac gcctgagcag tggaagtccc acagaagcta cagctgccag
7321 gtcacgcatg aagggagcac cgtggagaag acagtggccc ctacagaatg ttcataggtt
7381 ctcaaccctc acccccacca cgggagacta gagctgcagg atcccagggg aggggtctct
7441 cctcccaccc caaggcatca agcccttctc cctgcactca ataaaccctc aataaatatt
7501 ctcattgtca atcagaaatc ttgttttatc tcattttttc ttttctcaca tataattcct
7561 agcctttcgt gggttctcaa tttgtggtgg aaagaaccct gaacccagtg ggaaagttgc
7621 ctatgtgaag gggttctcag ttccctgggc atctctgcag gtaaggcctt cctcacccag
7681 acaccccttc ctcagctctc cactgtaccc ctgagccacc agcctcgcct ggctgggacc
7741 aggggggtgt cacactctcc tagattctgc ctttcaacag aaacctaacc acgcatcaca
7801 cggcacttct cgcatgcctt ctgtgtctgc tccagtctct gggctaaaga gttgctggtc
7861 cgggacaggg gataggtccg ctcttggtca gatgccaggt ccctgccatg gcatccctga
7921 ccctatgcaa caagccagtg actctggtga gctctctgtg tcaggagaat ccatgatcca
7981 gagtttcata ttgtcctgca agcatctggt gggctgtagc tcttgccaaa ctgggaaata
8041 ccatggccca gcatcaggat gcaggacagt ccggagaggg aaatcaggag aagtgaaggg
8101 gtctctgggg agcccagatg tgggctagag gcagaagtaa gggtgaagag cacctatgag
8161 tcaatgtcat ggtctcagca ggaacacagt tgaaaatccc cattccacac gagaccgttt
8221 agcaggaaag gagtccatac ttgtgctgcc accaggatgt cctgagaagc cttggagaat
8281 gaaacataca ggtgcatttc ctagacttga caatgcacgt tagccaagta aaggcaatga
8341 aaagttctct actagggaaa taatttcctg tggtaaagct t
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