GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS TATLFC1 3467 bp ss-mRNA INV 27-MAY-1991
DEFINITION Horseshoe crab limulus factor C mRNA, complete cds.
ACCESSION D90271
KEYWORDS complement; endotoxin; epidermal growth factor; lectin;
limulus factor C; serine protease.
SOURCE Horseshoe crab adult hemocytes amebocyte, cDNA to mRNA, clones
lambda FC[53 and 41].
ORGANISM Tachypleus tridentatus
Eukaryota; Animalia; Metazoa; Arthropoda; Chelicerata; Merostomata;
Xiphosura; Xiphosurida; Limulidae.
REFERENCE 1 (bases 1 to 3467)
AUTHORS Muta,T., Miyata,T., Misumi,Y., Tokunaga,F., Nakamura,T., Toh,Y.,
Ikehara,Y. and Iwanaga,S.
TITLE Limulus factor C: An endotoxin-sensitive serine protease zymogen
with a mosaic structure of complement-like, epidermal growth
factor-like, and lectin-like domains
JOURNAL J. Biol. Chem. 266, 6554-6561 (1991)
STANDARD full staff_review
COMMENT These data kindly submitted in computer readable form by:
Tatsushi Muta
Department of Molecular Biology
Graduate School of Medical Science
Kyushu University
33 Higashi-ku
Fukuoka 812
Japan
Phone: 092-641-1101 x4447
Fax: 092-632-2741
FEATURES Location/Qualifiers
CDS 45..3104
/note="limulus factor C precursor"
sig_peptide 45..119
/note="limulus factor C signal peptide"
mat_peptide 120..3101
/note="limulus factor C"
misc_feature 120..2114
/note="heavy chain"
misc_feature 348..452
/note="EGF-like domain"
misc_feature 468..629
/note="suchi domain (=SCR)"
misc_feature 641..806
/note="suchi domain (=SCR)"
misc_feature 822..1007
/note="suchi domain (=SCR)"
misc_feature 1350..1748
/note="lectin-like domain"
misc_feature 1515..1516
/note="alternative splicing site"
misc_feature 1611..1613
/note="put. glycosylation site"
misc_feature 1644..1646
/note="put. glycosylation site"
misc_feature 1770..1946
/note="suchi domain (=SCR)"
misc_feature 1914..1916
/note="put. glycosylation site"
misc_feature 1962..2288
/note="suchi domain (=SCR)"
misc_feature 2115..3101
/note="light chain"
misc_feature 2115..2330
/note="A chain"
misc_feature 2331..3101
/note="B chain"
misc_feature 2331..3101
/note="serine protease domain"
misc_feature 2343..2345
/note="put. glycosylation site"
misc_feature 2778..2780
/note="put. glycosylation site"
misc_signal 3433..3438
/note="polyadenylation signal"
misc_feature 3467..3467
/note="polyadenylation site"
BASE COUNT 951 a 718 c 839 g 959 t
ORIGIN
1 caggtttaac gcgaacgtgg aagaactctg aaggtaactt aagtatggtc ttagcgtcgt
61 ttttggtgtc tggtttagtt ctagggatac tagcccaaca aatgcgtcca gttcagtcca
121 gaggagtaga tctgggcttg tgtgatgaaa cgaggttcga gtgtaagtgt ggagatccag
181 gctatgtgtt caacgtccct atgaaacaat gcacgtactt ctatcgatgg aggccttatt
241 gtaaaccatg tgatgacctg gaggctaagg acatttgtcc aaagtacaaa cgatgtcaag
301 agtgtaaggc tggtcttgat agttgtgtta cttgtccacc taacaaatat ggtacttggt
361 gtagcggtga atgtcaatgt aagaatggag gtatctgtga ccagaggaca ggagcttgta
421 cctgtcgtga cagatatgaa ggagcgcact gtgaaattct caaaggttgt cctcttcttc
481 catcggattc tcaagttcag gaagtcagaa acccaccaga taatccccaa actattgact
541 acagctgttc accagggttc aagcttaaag gcgtggcacg aattagctgt ctcccaaatg
601 gacagtggag tagctttcca cccaaatgta ttcgagaatg tgccaaggtt tcatctccag
661 aacacgggaa agtgaatgct cctagtggca atatgataga aggggctact ttacggttct
721 catgtgatag tccctactac ttgattggtc aagaaacatt aacctgccag ggtaatggtc
781 agtggagtgg acaaatacca caatgtaaga agttggtctt ctgtcctgac cttgatcctg
841 taaaccatgc tgaacaccag gttaaaattg gtgtggaaca aaaatatggt cagtttcctc
901 aaggcactga agtgacctat acgtgttcgg gtaactactt cttgatgggt tttaacacct
961 taaaatgtaa ccctgatggg tcctggtcag gatcacagcc atcctgtgtt aaagtggcag
1021 acagagaggt cgactgtgac agtaaagctg tagacttctt ggatgatgtt ggtgaacctg
1081 tcaggatcca ctgtcctgct ggctgttctt tgacagctgg tactgtgtgg ggtacagcca
1141 tataccacga actttcctca gtgtgtcgtg cagccatcca tgctggcaag cttccaaact
1201 ctggaggggc ggtgcatgta gtgaacaatg gcccctactc ggactttctg ggtagtgacc
1261 tgaatgggat aaaatcggaa gagttgaagt ctcttgcccg cagttttcga tttgattatg
1321 tcagttcatc cacagcaggt agatcaggat gtcctgatgg atggtttgag gtagaagaga
1381 actgtgtgta cgttacatca aaacagagag cctgggaaag agctcaaggt gtgtgtacca
1441 atatggctgc tcgtcttgct gtgctagaca aagatctaat tccgagttcc ttgactgaga
1501 ctctacgagg gaaagggtta acaaccacat ggataggatt gcacagacta gatgctgaga
1561 agccctttgt ttgggagcta atggatcgta gtaatgtggt tctgaatgat aacctaacat
1621 tctgggcctc tggcgaacct ggaaatgaaa ctaactgtgt atatctggac atccgagatc
1681 agctgcagcc tgtgtggaaa accaagtcat gttttcagcc ctcaagcttt gcttgcatga
1741 tggatttgtc agacagaaat aaagccaaat gcgatgaccc tggaccactg gaaaatggac
1801 acgccacact tcatggacaa agtattgatg ggttctatgc tggttcttct ataaggtaca
1861 gctgtgaggt tctccactac ctcagtggaa ctgagaccgt aacttgtaca acaaatggca
1921 catggagtgc tcctaaacct cgatgtatca aagtcatcac ctgccaaaac cctcctgtac
1981 catcatatgg ttctgtggaa atcaaacccc caagtcggac aaactcgatc agtcgtgttg
2041 ggtcaccttt cttgaggttg ccacggttac ccctcccatt agccagagca gccaaacctc
2101 ctccaaaacc tagatcctca caaccctcta ctgtggactt ggcttctaaa gttaaactac
2161 ctgaaggtca ttaccgggta gggtctcgag ccatttacac gtgcgagtcg agatactacg
2221 aactacttgg atctcaaggc agaagatgtg actctaatgg aaactggagt ggtcggcccg
2281 ctagctgtat tccagtttgt ggacggtcag actctcctcg ttctcctttc atctggaatg
2341 ggaattctac agaaataggt cagtggccgt ggcaggcagg aatctctcga tggcttgcag
2401 accacaatat gtggtttctc cagtgtggag gatccctatt gaatgagaaa tggatcgtca
2461 ctgctgccca ctgtgtcacc tactctgcta ctgctgagat aattgatccc agtcagttta
2521 aaatctatct gggcaagtac taccgtgatg acagtagaga cgatgactac gtacaagtaa
2581 gagaggctct cgagatccac gtaaatccta actacgaccc cggcaatctc aactttgaca
2641 tagccctaat tcaactgaaa actcctgtta ctttgacaac acgagtccaa ccaatctgtc
2701 tgcctactga catcacaaca agagaacact tgaaggaggg aacattagca gtggtgacag
2761 gttggggttt gaatgaaaac aacacatatt cagagatgat tcaacaagct gtgctacctg
2821 ttgttgcagc aagcacctgt gaagaggggt acaaggaagc agacttacca ctgacagtaa
2881 cagagaacat gttctgtgca ggttacaaga agggacgtta tgatgcctgc agtggggaca
2941 gtggaggacc attagtgttt gctgatgatt cccgtaccga aaggcggtgg gtcttggaag
3001 ggattgtcag ctggggcagt cccagtggat gtggcaaggc taaccagtat gggggcttca
3061 ctaaagttaa cgtttttcta tcatggatta ggcagttcat ttgaaactga tctaaatatt
3121 ttaatcatgg ttataaacgt cttgtttcct atttttgctt tactagttta acccataaga
3181 aggttaagtg ggtaaggcac cagtgtcatt gtttgtttgt ttttacaaat ggttcgttta
3241 gtcaatgaat gagaatagta tccattggac actgttacct tttatgtttt tattctacct
3301 ttttatatta ccatgcaagt atttggaata tcttctatac atatgaaaat tctgttattt
3361 ttccataaag ttggtttctg gtgtgcgtta agtccaccac tggagaatga tgtaattttc
3421 actagtacat gaaataaata tagaacaaat ctattataaa ctacctt
//