GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS AZSGLNB 975 bp ds-DNA BCT 27-MAY-1991
DEFINITION Azospirillum brasilense glnB gene
ACCESSION X51499
KEYWORDS glnB gene; protein PII.
SOURCE Azospirillum brasilense DNA.
ORGANISM Azospirillum brasilense
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic/microaerophilic, motile, helical/vibrioid bacteria.
REFERENCE 1 (bases 1 to 975)
AUTHORS de,Z.M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 975)
AUTHORS de,Z.M., Delorme,F. and Elmerich,C.
TITLE Characterisation of three different nitrogen-regulated promoter
regions for the expression of glnB and glnA in Azospirillum
brasilense
JOURNAL Mol. Gen. Genet. 224, 421-430 (1990)
STANDARD full automatic
COMMENT *source: strain=SP7; For nifH gene see <X51500>.
From EMBL entry ABGLNB; dated 07-JAN-1991.
FEATURES Location/Qualifiers
promoter 147..151
/note="Canonical E.coli type promoter of gluB-A"
misc_feature 169..173
/note="Consensus sequence for strong NtrC binding site"
promoter 176..180
/note="Canonical E.coli type promoter of gluB-A"
misc_feature 179..185
/note="Consensus sequence for strong NtrC binding site"
promoter 281..297
/note="NtrA-dependent promoter of gluB-A"
RBS 358..362
/note="ribosome binding site for glnB"
CDS 366..701
/note="glnB gene product (AA 1-112)"
/codon_start=366
misc_feature 850..893
/note="AT-rich stretch upstream of glu A"
promoter 859..875
/note="glnA promoter"
RBS 970..974
/note="ribosome binding site for glnA"
BASE COUNT 205 a 315 c 300 g 155 t
ORIGIN
1 gagctctacc aactgagcta ccaccgccac cggggtcgcc tatttgcccc aagggacggg
61 gcgggtcaag ctctattttc caccttgtcc atcaatgtga aaagccgggt ggaaagccgg
121 cgctacccca tccgaacgga caaggctgcg cggattgctt gcagcatgtg cacaaatatt
181 gttcacttcg ccctatccgt tggcagtcgc gtgccgccca cggtggcatc ggcccgctgc
241 ggcccggcat cccacgcagc cggatcgcaa ccgctccgat tggcacgcaa cgtgctttac
301 atcggaccgt cggcagaggc ctggacggtg cctgccgacg ttggcacctc gtacacgaga
361 gacccatgaa gaagatcgaa gccatcatta agccgttcaa actcgacgaa gtgaaggaag
421 cccttcacga agtcggcatc aagggcatca ccgtcaccga ggccaagggc ttcggccgtc
481 agaaggggca caccgagctg taccgcggcg cggagtatgt ggtcgacttc ctgccgaagg
541 tgaagatcga ggtggtgatg gaggactccc tggtggagcg ggcgatcgag gcgatccagc
601 aggccgccca caccggccgc atcggcgacg gcaagatctt cgtcaccccc gtggaagaag
661 ttgtccgcat ccggaccggc gagaagggag gcgacgcgat ctgatcgcgt cccgagaacc
721 cgaccaccgg ccaccatgaa ccagccccgc ccacaaccga cgcagagccg gccgactccg
781 atcgggccga cgcagaccgt cgcaacgcga gcggaacgat ccgtgcgtgg atgcccatcc
841 gggcgcgact ttttgtgctg tcacgttgtg tgcttgggaa aggcatgaca taacgtccgc
901 cgcgagcgcc gtcacccgcc ccttggtcgg tttgacgcag cgggacccaa acctcttaag
961 cgtggaaaaa gagat
//