GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS AZSNIFH 1826 bp ds-DNA BCT 27-MAY-1991
DEFINITION Azospirillum brasilense NifH gene
ACCESSION X51500
KEYWORDS nifH gene; nitrogenase; protein II.
SOURCE Azospirillum brasilense DNA.
ORGANISM Azospirillum brasilense
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic/microaerophilic, motile, helical/vibrioid bacteria.
REFERENCE 1 (bases 1 to 1826)
AUTHORS de,Z.M., Delorme,F. and Elmerich,C.
TITLE Regulation of transcription and promoter mapping of the structural
genes for nitrogenase (nifHDK) of Azospirillum brasilense SP7
JOURNAL Mol. Gen. Genet. 220, 88-94 (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1826)
AUTHORS de,Z.M.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17303; NIFH$AZOBR.
*source: strain=Sp7; For glnB gene see <X51499>.
From EMBL entry ABNIFH; dated 20-JUN-1990.
FEATURES Location/Qualifiers
misc_feature 530..532
/note="first UAS signal for NifA protein"
misc_feature 539..541
/note="second UAS signal for NifA protein"
misc_feature 543..545
/note="first UAS signal for NifA protein"
misc_feature 552..554
/note="second UAS signal for NifA protein"
misc_feature 576..616
/note="AT rich region for UAS signal"
misc_feature 623..639
/note="NtrA dependent promoter of NifH"
misc_feature 649..649
/note="first transcription start site upstream of nifH"
misc_feature 683..683
/note="second transcription start site upstream of nifH"
RBS 707..711
/note="nifH ribosome binding site"
CDS 721..1599
/note="nifH gene product (AA 1-293)"
/codon_start=721
misc_feature 1619..1714
/note="potential termination of nifH transcription"
RBS 1729..1733
/note="nifD ribosome binding site"
misc_feature 1743..1745
/note="start codon of nifD gene"
BASE COUNT 343 a 619 c 573 g 291 t
ORIGIN
1 aacgccttcg tccgagcccg ccgacccgca aggcgatgaa cgattgcctg tcgcgctgcg
61 gctttccgac acgctgttcg atcttgtcgg aaaggcggcc aacgcgcgac ctccgacgag
121 ctgctgttcg aggcgctgct gcgccagctc cgttccctgc accagttctg cacggatctc
181 gaccatgccg ggcgtccgcg ggtgctcgac acgctggaac gccgtctggc cgaacgcagc
241 cgcgcgctgg ccggcatcgc cggggagcgc ctcgtcggct cgtcctgctg gcccgccccg
301 ccgacccggc caaggcgcgc cgcctgctcg cccgcggcca gtcgctggcc cagcttctct
361 atgacatggg ccaggacggc gacgagttgg aggcgttggc gctgcgcctc gtcgtcgccc
421 gcgacgcgct gaaccacgcc gccgcctgac gctggctctg acgctggcgg accgacattc
481 gtccatccct tccttttcat tgcgagagac ccgacaaaat gtcgggcttt gtcgggtatg
541 tcacaggccc gacaaagcgg accgcgctcg tccgcaatca attttcttgt tcaatttcaa
601 tgatttaaaa atttttcgcg aactggcacg ggggatgcag agaaggggtc gaagcggccg
661 ctggcaggcc gccccggaat tgaagacacc ctgtagaccc aagcaaagga gtaacctccc
721 atgtctttgc gccagattgc gttctacggt aagggcggta tcggcaagtc caccacctcc
781 cagaacaccc tggccgcgct ggtcgagctg gatcagaaga tcctgatcgt cggctgcgat
841 ccgaaggccg actcgacccg cctgatcctg cacgccaagg cgcaggacac cgtgctgcac
901 ctcgccgccg aagccggctc ggtcgaggat ctggagctcg aggacgttct caagatcggc
961 tacaagggca tcaagtgcgt cgagtccggc ggtccggagc cgggggtcgg ctgcgccggc
1021 cgcggcgtga tcacctcgat caacttcctg gaagagaacg gcgcctacga cgacgtggac
1081 tacgtctcct acgacgtgct gggcgacgtg gtgtgcggcg gtttcgccat gcccatccgc
1141 gagaacaagg cccaggaaat ctacatcgtc atgtccggtg agatgatggc gctctacgcc
1201 gccaacaaca tcgccaaggg cattctgaag tacgcccaca gcggcggcgt gcgcctcggc
1261 ggcctgatct gcaacgagcg ccagaccgac aaggaaatcg acctcgcctc cgccctggcc
1321 gcccgcctcg gcacccagct catccacttc gtgccgcgcg acaacatcgt gcagcacgcc
1381 gagctgcgcc gcatgacggt gatcgagtac gcgccggaca gccagcaggc ccaggaatac
1441 cgccagctcg ccaacaaggt ccacgcgaac aagggcaagg gcaccatccc gaccccgatc
1501 acgatggaag agctggaaga gatgctgatg gacttcggca tcatgaagtc ggaggagcag
1561 cagctcgccg agctccaggc caaggaagcc gccaaggcct gataactggg tcccccacgg
1621 ggcgcagttc tgaactggcc ccctccccag cccaccccgc ccctgtgcgc ggggtgggag
1681 agagggggct cggtcccgcg ctgcagtagc gcggataatg agtgcagcag gagactggca
1741 ccatgagcct gtccgtgaac gaaggcgtcg acgtcaaggg tctcgtcgac aaggttctcg
1801 aagcgtatcc cgagaagtcg cgcagg
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