GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS AVHNIFI 1654 bp ds-DNA BCT 27-MAY-1991
DEFINITION Azotobacter chroococcum MCD 1 nifH* gene for nitrogenase iron
protein
ACCESSION X03916 M24596
KEYWORDS ferredoxin; nitrogenase.
SOURCE Azotobacter chroococcum DNA.
ORGANISM Azotobacter chroococcum
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Azotobacteraceae.
REFERENCE 1 (bases 1 to 1654)
AUTHORS Robson,R., Woodley,P. and Jones,R.
TITLE Second gene (nifH) coding for a nitrogenase iron protein in
Azotobacter chroococcum is adjacent to a gene coding for a
ferredoxin-like protein
JOURNAL EMBO J. 5, 1159-1163 (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P06118; NIF2$AZOCH. SWISS-PROT; P06123; FERV$AZOCH.
Data kindly reviewed (29-JUL-1986) by R. Robson
From EMBL entry ACNIFH; dated 30-MAR-1990.
FEATURES Location/Qualifiers
promoter 272..287
/note="pot. nif promoter"
CDS 338..1207
/note="nitrogenase (nifH*) (aa 1-290)"
/codon_start=338
CDS 1335..1529
/note="put. ferredoxin"
/codon_start=1335
terminator 1598..1633
/note="stem-loop structure pot. transcription terminator"
BASE COUNT 342 a 555 c 482 g 275 t
ORIGIN
1 cagacacgaa gaagccgggc ccgtgacatg cccgccatgg actgctgctc cgtcgccgca
61 cgccacttcc tgcaccagcc ggcatgaacc ccggtaccac atgggaacgg atcgccgcgg
121 cgttactacc ggtacgccgc cagcccggga cgacgcagat cgctgccgtc cgactcccga
181 cacatgccat atgcagcatg aaatatcgct gaaaacatat tactggtttt tttatccaaa
241 aaacaaacaa catatgaaat tcacatcttg atggcaccac ccttgctcca tcccctgcga
301 caccagtcaa acgccacgaa tcaatggagg ttccaagatg gcattgcgtc agtgtgcaat
361 ttacggcaag ggtggtatcg gcaagtccac caccacccag aacctggtcg ccgcgctcgc
421 cgaggccggc aagaaggtga tgatcgtcgg ttgcgacccg aaagccgact ccacccgcct
481 gatcctgcat tccaaggccc agaacaccgt catggagatg gccgcatccg ccggctcggg
541 tgaagacctc gagctggaag acgtgctgca gatcggctac ggcggcgtca agtgcgtcga
601 gtccggcggc cctgagccgg gcgtcggctg cgcgggccgt ggcgtgatca ccgcgatcaa
661 cttcctggaa gaggaaggcg cctacagcga cgacctggac ttcgtgttct acgacgtgct
721 gggcgacgtg gtgtgcggcg gcttcgccat gccgatccgc gagaacaagg cccaggaaat
781 ctacatcgtc tgctccggcg agatgatggc catgtacgcc gccaacaaca tcgccaaggg
841 catcgtgaag tacgcccact ccggcagcgt gcgtctgggc gggctgatct gcaacagccg
901 caagaccgac cgcgaagacg agctgatcat ggccctggcc gcgaagatcg gcacccagat
961 gatccacttt gtgccgcgcg acaacgtcgt gcagcacgcc gaaatccgcc gcatgaccgt
1021 gatcgaatac gatccgaaag ccaagcaggc cgacgagtac cgtgccctgg cccagaagat
1081 cctcaacaac aagctgctgg tcatcccgaa cccggcgagc atggaggacc tcgaagagct
1141 gctgatggag ttcggcatca tggaagccga agacgagtcc atcgtcggca aggccggcgc
1201 cgagggctga tcccgccggc gcagtgtttg cggaggagcg tgcgtcgcgg gctgtccgga
1261 atggcttctc gcggccggca cgccgccctc ccttttgaat cgccccgaat tctccaacct
1321 caggagctga ccctatggcc atggccatcg acggctacga atgcaccgtc tgcggcgact
1381 gcaagccggt ctgcccgacc ggctcgatcg tcctccaggg cggtatctac gtgatcgacg
1441 ccgacagctg caacgagtgc gccgacctgg gcgagccacg ctgtctcggc gtctgccccg
1501 tggacttctg catccagccg ctcgatgact gaagactgaa cgagccgcac ccgcttgccg
1561 gcgacagagc atcccgccgc tctgccaccg gaccaccaaa cggcgatcgc tttcctcagg
1621 gcgatcgccg ttttttctct cctgaccgca cctt
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