GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS AVHVNF 7203 bp ds-DNA BCT 27-MAY-1991
DEFINITION Azotobacter chroococcum vnfH, D, G, and K genes, region encoding
the structural genes for vanadium nitrogenase
ACCESSION X51756
KEYWORDS VFe protein; ferredoxin-like protein; nitrogen fixation;
nitrogenase; unidentified reading frame; vanadium nitrogenase;
vnfD gene; vnfG gene; vnfH gene; vnfK gene.
SOURCE Azotobacter chroococcum DNA.
ORGANISM Azotobacter chroococcum
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Azotobacteraceae.
REFERENCE 1 (bases 1 to 7203)
AUTHORS Robson,R.L.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 7203)
AUTHORS Fallik,E. and Robson,R.L.
TITLE Completed sequence of the region encoding the structural genes for
the vanadium nitrogenase of Azotobacter chroococcum
JOURNAL Nucleic Acids Res. 18, 4616-4616 (1990)
STANDARD full automatic
COMMENT See <X03916> and <X15077> for overlapping sequences.
From EMBL entry ACVNF; dated 10-AUG-1990.
FEATURES Location/Qualifiers
CDS 337..1206
/note="vnfH gene product (AA 1-290)"
/codon_start=337
CDS 1334..1528
/note="ferredoxin-like protein (AA 1-65)"
/codon_start=1334
CDS 1701..3248
/note="ORF (AA 1-516)"
/codon_start=1701
CDS 3655..5076
/note="vnfD gene product (AA 1-474)"
/codon_start=3655
CDS 5079..5417
/note="vnfG gene product (AA 1-113)"
/codon_start=5079
CDS 5475..6899
/note="vnfK gene product (AA 1-475)"
/codon_start=5475
BASE COUNT 1443 a 2489 c 2135 g 1136 t
ORIGIN
1 cagacacgaa gaagccgggc ccgtgacatg cccgccatgg actgctgctc cgtcgccgca
61 cgccacttcc tgcaccagcc ggcatgaacc ccggtaccac atgggaacgg atcgccgcgg
121 cgttactacc ggtacgccgc cagcccggga cgacgcagat cgctgccgtc cgactcccga
181 cacatgccgg ttatgcagca tgaatatcgc tgaaacatga ttactggttt tttatccaaa
241 aacaaacaac atatgaaatt cacatcttga tggcaccacc cttgctccat cccctgcgac
301 accagtcaaa cgccacgaat caatggaggt tccaagatgg cattgcgtca gtgtgcaatt
361 tacggcaagg gtggtatcgg caagtccacc accacccaga acctggtcgc cgcgctcgcc
421 gaggccggca agaaggtgat gatcgtcggt tgcgacccga aagccgactc cacccgcctg
481 atcctgcatt ccaaggccca gaacaccgtc atggagatgg ccgcatccgc cggctcgggt
541 gaagacctcg agctggaaga cgtgctgcag atcggctacg gcggcgtcaa gtgcgtcgag
601 tccggcggcc ctgagccggg cgtcggctgc gcgggccgtg gcgtgatcac cgcgatcaac
661 ttcctggaag aggaaggcgc ctacagcgac gacctggact tcgtgttcta cgacgtgctg
721 ggcgacgtgg tgtgcggcgg cttcgccatg ccgatccgcg agaacaaggc ccaggaaatc
781 tacatcgtct gctccggcga gatgatggcc atgtacgccg ccaacaacat cgccaagggc
841 atcgtgaagt acgcccactc cggcagcgtg cgtctgggcg ggctgatctg caacagccgc
901 aagaccgacc gcgaagacga gctgatcatg gccctggccg cgaagatcgg cacccagatg
961 atccactttg tgccgcgcga caacgtcgtg cagcacgccg aaatccgccg catgaccgtg
1021 atcgaatacg atccgaaagc caagcaggcc gacgagtacc gtgccctggc ccagaagatc
1081 ctcaacaaca agctgctggt catcccgaac ccggcgagca tggaggacct cgaagagctg
1141 ctgatggagt tcggcatcat ggaagccgaa gacgagtcca tcgtcggcaa ggccggcgcc
1201 gagggctgat cccgccggcg cagtgtttgc ggaggagcgt gcgtcgcggg ctgtccggaa
1261 tggcttctcg cggccggcac gccgccctcc cttttgaatc gccccgaatt ctccaacctc
1321 aggagctgac cctatggcca tggccatcga cggctacgaa tgcaccgtct gcggcgactg
1381 caagccggtc tgcccgaccg gctcgatcgt cctccagggc ggtatctacg tgatcgacgc
1441 cgacagctgc aacgagtgcg ccgacctggg cgagccacgc tgtctcggcg tctgccccgt
1501 ggatttctgc atccagccgc tcgatgactg aagactgaac gagccgcacc cgcttgccgg
1561 cgacagagca tcccgccgct ctgccaccgg accaccaaac ggcgatcgct ttcctcaggg
1621 cgatcgcgtt ttctctcttc gatgacgcac cttccgggcg gctacaatcg cagcacaccc
1681 caccacggta ctgcctgccc atgtcccgca agaagctgcc catcggcatc cagaccttcg
1741 ccaagatccg cgaaggcgac gactactact acgtcgacaa gaccggcttc gccctgcaac
1801 tgatcgagca gggcacgcac tacttcctct cccggccgcg gcgcttcggc aagagcctgt
1861 tcctcgacac cctggccgag ctgttcgccg gcaacgagcc gctgttccgc gggctgcagg
1921 tgcatgaccg ctgggactgg agccggcgcc atccggtgct gcggatcagc ttcggcggcg
1981 gggtgattcg cgatgcgcag gacctggagg gcaagatcgc cgagctgctg acgatcaacg
2041 agcaggccct gggcatcgcc tgtacgcagc gcgagaaccg tgcccgcttc ggcgagctga
2101 tccgccaggc gcatgccgcc ggcggcgagc gggtggtggt gctggtcgac gagtacgaca
2161 agccgatcct cgacaacctc acccgcccgg agatcgcccg cgagatccgc gacgggctgc
2221 gcaacctgta ctcggtgatc aaggattccg acgcgcacgt ccgcttcgcc atgctcagcg
2281 gcgtgtcgaa gttcagcaag gtcagcctgt tctccggtct gaacaacctc gacgacatca
2341 gcgtcgacag tcgctattcg gcgctctgcg gctacaccga gacggacgtg gatgcggtgt
2401 tcgcccccga gctgccgggc ctcgaccgcg acgagatccg cgcctggtac aacggctaca
2461 actggaccgg cgaggcggtc tacaacccct tcgacctgct gctgctgttc cagaagcgcg
2521 agttccgccc ctactggttc gagacgggca caccgacctt cctggtcgac ctgctcaccg
2581 agcggcgggc cttcaccccg gccctcgaac aggtcatggc caccgacgcg ctgctctcgg
2641 ccttcgaggt cggcgacatt tccaccgagg cactgatgtt ccaggccggc tacctgacca
2701 tcggccaggt gcagcgcatc ggcgcgcaac cgcgctaccg gctgcgctat ccgaacctcg
2761 aagtgaaggc cagcctcaac gaggcgctgc tgctgcgcta cctgccggag cccgccagcg
2821 tgatgcgcca gaccgggcag ctctacgacc tgctgctggc caatgacttt cccggcctgc
2881 agacactgtt caccgccttc ttcgccagca ttccccacga ctggtaccgc aacaatccca
2941 tcgcccgcta cgagggctac tacgccagcg tgttctacag ccacttcgcc gcgctgggcc
3001 tggacgttcg cctggaagac gcgaccagcc acggccgcat cgacatgacg gtgctgttca
3061 acggtcatgt ctacctgttc gagttcaagg tggtcgagct gacgcccgca gggcaggcgc
3121 tgcaacaact caaggacaaa ggctacgccg acaagtaccg cgcccgcggc gagcccatcc
3181 atctgctcgg cgtggagttc agccgcgaca gtcgcagcgt ggtgggattc gtggtggaga
3241 gcctgggctg agcagcccct ctccccggcc ctctccctca cggggaaagg gagatttccc
3301 ctccaaccga cagccacgca cctcgacgta cagaccccga acgctccgta cttggccgaa
3361 cgaaaaacct tttgcgtatt ccgccggccg tccgcgccag cgctgcaaac gcggaaaaaa
3421 cgcccgaaaa gccccgcgcg acgcctttct gcagcagcag aagaaaccaa caagtcattg
3481 attcaaaaga attaatcatg tcgctcggcg ggcgaacgga aaattcccgc aagcgccgca
3541 agggcatctg gcacgcatcc tgccatttcc tcttcgccaa ccggtttttc cggtccccga
3601 tcgagtgccg gtcacggggt tcgatcaacg catagagccg aggagacttc aaccatgcca
3661 atggtattgc tggaatgtga caaggacata cccgagcgcc agaaacacat ctatctgaag
3721 gcgcccaacg aggacacccg cgagttcctg ccgatcgcca acgcggcgac catccccggc
3781 accctgtccg aacgcggctg cgccttctgc ggcgccaagc tggtgatcgg cggcgtgctc
3841 aaagacacca tccagatgat tcacggcccg ctcggctgtg cctacgacac ctggcacacc
3901 aagcgctacc cgaccgacaa cggccacttc aacatgaagt acgtctggtc gaccgacatg
3961 aaggaaagcc atgtggtgtt cggcggcgag aagcgcctcg agcagcgcat gcacgaagcc
4021 ttcgacgaga tgcccgacat caagcggatg atcgtctaca cgacctgccc gaccgcgctg
4081 atcggcgacg acatcaaggc cgtggccaag aaggtgatga aggagcgtcc cgacgtggac
4141 gtcttcaccg tcgaatgccc cggcttctcc ggcgtgtccc agtccaaggg ccaccacgtc
4201 ctgaacatcg gctggatcaa cgagaaagtc gagacgatgg agaaggagat caccagcgaa
4261 tacacgatga acttcatcgg tgacttcaac atccagggcg atacccagct gctgcagacc
4321 tactgggacc gcctgggcat ccaggtcgtc gcccacttca ccggcaacgg cacctacgac
4381 gacctgcgct gcatgcacca ggcccagctc aacgtggtga actgcgcgcg ctcctccggc
4441 tacatcgcca acgaactgaa gaagcgctac ggcatcccgc gcctggacat cgactcctgg
4501 ggcttcagct acatggccga gggcatccgc aagatctgcg ccttcttcgg catcgaggag
4561 aagggcgaac ggctgatcgc cgaggaatac gccaagtgga agccgaagct cgactggtac
4621 aaggagcgcc tgcagggcaa gaagatggcg atctggaccg gcggcccgcg cctgtggcac
4681 tggaccaagt cggtcgagga cgacctaggc atccaggtgg tggccatgtc ctccaagttc
4741 ggccatgagg aagacttcga gaaggtcatc gcccgcggca aggaaggcac ctactacatc
4801 gacgacggca acgagctgga gttcttcgag atcatcgacc tggtcaagcc ggacgtgatc
4861 ttcacaggtc cccgcgtcgg cgagctggtc aagaagctgc acatccccta cgtcaacggc
4921 cacggctacc acaacggccc gtacatgggc ttcgagggct tcgtcaacct cgcccgcgac
4981 acctacaacg ccgtgcacaa cccgctgcgc cacctggccg ccgtggacat ccgcgacagc
5041 tcgcagacca ctccggtcat cgtgcggggg gccgcctgat gagccagtcc catctcgacg
5101 acctgttcga ctacaccgag gagcgctgcc tgtggcagtt cttctcgcgc acctgggacc
5161 gcgaggaaaa catcgagggc gtgctcggcc aggtcgcccg cctgctgacc ggccaggagc
5221 cgctgcgcgg caccccgcag gagcgcctgt tctacgccga cgccctggcc atggccaacg
5281 acgttcgcga gcgcttcccc tgggcatcgc agatcaatca cgaggaaatc catttcctga
5341 tcgacggcct caagtcccgc ctggtcgaca ccgtgatcca aagctcgacc aaccgcgaac
5401 tcaaccacca cctctactga gtcaccgctg cgggagcggc ccgagggccg tcccctgtgc
5461 gaaggagcca tttcatgagc aattgcgagc taaccgtgct gaagccggct gaagtcaagc
5521 tggtcaagcg ggagcgcgaa ggcatcatca acccgatgta cgactgccag cccgcgggcg
5581 cccagtacgc cggcatcggc gtcaaggact gcatcccgct ggtccacggc ggccagggct
5641 gcacgatgtt cgtccgcctg ctgttcgccc agcacttcaa ggaaaacttc gacgtcgcct
5701 cgacctcgct gcacgaggag tcggcggtgt tcggcggcgc caagcgcgtc gaggaaggcg
5761 tgctggtgct ggcccgccgc tacccggaac tgcggctgat cccgatcatc accacctgct
5821 ccaccgaggt catcggcgac gacatcgagg gcaccatcaa cgtctgcaac cgggcgctcg
5881 ccgccgagtt cccggagcgc aagatctacc tggcgccggt gcacaccccg agcttcaagg
5941 gcagccacgt caccggctac gccgagtgcg tgaagtcgat gttcaagacc atcaccgagg
6001 tgcacggcaa gggccagccg agcggcaagc tcaacgtctt cccgggctgg gtcaaccccg
6061 gcgacgtggt gctgctcaag cgctacttca aggaaatggg cgtcgacgcc acggtgttca
6121 tggacaccga ggacttcgac tcgccgatgc tgccgaacaa gagcatcgag acccacggcc
6181 gcaccacggt cgaggacatc gccgacagcg ccaacgccct ggccaccctg gccctggccc
6241 gctacgaggg cgcgaccacc ggcgagtacc tggagaagac cttcgcggtg ccgaacagcc
6301 tggtcaacac cccctacggc atcaagaaca ccgacgacat gctgcgcaag atcgccgaga
6361 tcaccggcaa ggagatcccc gagtcgctgg tccgcgagcc gcgcatcgcg tggatcgcgc
6421 tggccgacct ggcgcacatg ttcttcgcca acaagaaggt ggcgatcttc ggtcatccgg
6481 acctggtgct cggcctggcg cagttctgcc tggaagtgga actggaaccg gtgctgctgc
6541 tgatcggcga cgaccagggc agcaagtaca agaaggaccc gcgcctccag gagctcaagg
6601 acgccgcgca cttcgacatg gagatcgtcc acaacgccga cctctgggag ctggagaagc
6661 gcatcaacga cggcctgcag ctcgacctga tcatgggtca ctcgaagggc cgctacgtcg
6721 ccatcgaagc caacatcccg atggtccgcg tcggcttccc gaccttcgac cgcgccggcc
6781 tctaccgcaa gccgagcatc ggctaccagg gcgccatgga gctcggcgag atgatcgcca
6841 acgccatgtt cgcccacatg gaatacaccc gtaacaagga atggatcctc aatacgtggt
6901 gagttgctag gcggtcccct gctccggcag ggcctccgcc ccgtattcca ctccggccgc
6961 catgccgcct tcgggagaga gccatgaaac agcgacagga atggtcgcca ctatcgcgcg
7021 tgcttcggcg agctgtgcgc ccgaccggaa caccgcccca tcgaacccta cacccgcccg
7081 cggcgcctga gcttcgcgag ccggaaaccg acgccacccg ccgcctgccc ggccgcctgg
7141 tgctggcgct gaccagtgcc tacgcgctct tggccgactg caggagtgcc gcgatccgtc
7201 gct
//