GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS AFAMPCI 1805 bp ds-DNA BCT 27-MAY-1991
DEFINITION Alcaligenes eutrophus mpcI gene for metapyrocatechase (EC
1.13.11.2)
ACCESSION X52414
KEYWORDS catechol 2,3-dioxygenase; metapyrocatechase; mpcI gene.
SOURCE Alcaligenes eutrophus DNA.
ORGANISM Alcaligenes eutrophus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Alcaligenaceae.
REFERENCE 1 (bases 1 to 1805)
AUTHORS Kabisch,M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1805)
AUTHORS Kabisch,M. and Fortnagel,P.
TITLE Nucleotide sequence of metapyrocatechase I (catechol 2,3-oxygenase
I) gene mpcI from Alcaligenes eutrophus JMP222
JOURNAL Nucleic Acids Res. 18, 3405-3406 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17295; MPC1$ALCEU.
Data kindly reviewed (13-AUG-1990) by Kabisch M.
*source: strain=JMP222;
From EMBL entry AEMPCI; dated 01-OCT-1990.
FEATURES Location/Qualifiers
RBS 490..494
/note="ribosome binding site"
misc_feature 496..498
/note="pot. alt. start codon"
CDS 502..1440
/note="metapyrocatechase (AA 1-313)"
/codon_start=502
BASE COUNT 276 a 628 c 588 g 313 t
ORIGIN
1 ggatccgctg gccggctcgc ccgtggcgcg catgttcatc cagccgcgcg tacgtactgc
61 cgatgatcgc ctcgcgctgc tcgacgacgc catcggcaat ggttttgccg tgatcggctg
121 gggcaccaat ccgaccttcg gcctgacgcc gcaggcacgc gcgctcgccg agaaggccgg
181 catgcgcttc gtactcgcca agcctgatgt gcaactgcgt cacggcgacg acgtgcccga
241 tggggtgatc gcgatcggcg atgcaagcgg ccgcctgaag gaatggttca gccgcctgcc
301 gcagtcggtg gtcctgctgc gtccggatcg cttcgttgcg ggcgtgtgct cgccccagca
361 ggtgtcggag gccatcactg aactcgccgg caagctcggc atgacggagg tgccgcctta
421 tgccgaggcc tctacccctt ccaagacgtc gcgccccgtg ctcgaccccg tcaccgttac
481 ccaggcagca ggagcatgaa gatgccgatc caactggaat gtctgtcgca tacgccgttg
541 cacggctacg tcgaccccgc gcccgaagtc gtcgccgagg tcgagcgcgt acaggctgcg
601 gcgcgcgatc gcgtgcgtgc attcgatccc gaactggtcg tggtctttgc gccggaccac
661 ttcaacggct tcttctatga cgtgatgccg ccgttctgca tcggcgcagc cgccaccgcg
721 atcggtgact tcaagagcct ggccggcaag ctgcctgtgc cggcggacct cgctctgtcg
781 ctggccgaaa gcgtgatggc cgccgatatc gatgtcgcgc tgtcgcaccg catgcaggtc
841 gaccatggct gcgcggatgc gctcgcggca ttgaccggca gcctgcatcg ctatccggtc
901 atccccgtgt tcatcaattc cgtggcgccg ccgatggcga cactgcgccg cgcgcgcctg
961 ctcggcgatg cggtcggccg cttcctgtcg cgtgccggca agcgtgtgct ggtggtcggt
1021 tctggcggca tctcgcatga gccgccggtg ccggagttgg ccggcgccag cgaggaagtt
1081 gccgaacgcc tgatcgcagg acgcaacccg tcgcccgaat cggccgcgcg ccaggcccgc
1141 actgttgccg ccgcgaagtc tttcgtcgcg ggcgacagcc acctgcatcc gctcaatccc
1201 gagtgggacc gcgcattcct gtcgctgctc gcttccggtg aactcaccgc cgtcgatggc
1261 atgaccaacg acgcgatcac gcgcgacggc ggcaagtcgg cgcacgagat tcgcacgtgg
1321 gtggctgcat tcggtgcgct ggctgcgtac gggccgtatc gcgcatcgct cgatttctac
1381 cgcgcgattc ctgagtggat cgcgggcttc gccaccatgc atgccgagcc tgcagcggtc
1441 tgacccgatc aaggagaacc ccgatgactg aaccaaattt cgtcaacgcc atcgcagcac
1501 gcctgcgcga tgccgaggcc acgcgccaga ccgtcgcgcc ggtgcgtggc gagatcgcga
1561 tcgatgacat cgccaccgcg tatgccgtgc agcaggccaa cgtcgacgcg cgcctcgcac
1621 aaggcgaacg catcgtcggc cgcaagatcg gcctgacttc gctggccgtg cagaagcagc
1681 tcggcgtcga ccagcccgac ttcggcgcgc tgttcgcgag catggcgtat ggcgacagcc
1741 agccgatgcc gctgtcgcgc ctgatccagc cgaaggtcga agccgagatc gcactggtgc
1801 tcgag
//