GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS AFAMPCII 2020 bp ds-DNA BCT 27-MAY-1991
DEFINITION Alcaligenes eutrophus mpcII gene for metapyrocatechase (EC
1.13.1.2)
ACCESSION X52415
KEYWORDS catechol 2,3-dioxygenase; metapyrocatechase; mpcII gene.
SOURCE Alcaligenes eutrophus DNA.
ORGANISM Alcaligenes eutrophus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Alcaligenaceae.
REFERENCE 1 (bases 1 to 2020)
AUTHORS Kabisch,M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2020)
AUTHORS Kabisch,M. and Fortnagel,P.
TITLE Nucleotide sequence of the metapyrocatechase II (catechol 2,
3-oxygenase II) gene mpcII from Alcaligenes eutrophus JMP 222.
JOURNAL Nucleic Acids Res. 18, 5543-5543 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17296; MPC2$ALCEU.
*source: strain=JMP222; See <X52414> for mpcI gene.
From EMBL entry AEMPCII; dated 12-NOV-1990.
FEATURES Location/Qualifiers
promoter 271..276
/note="put. -35 region"
promoter 294..299
/note="put. -10 region"
precursor_RNA 307..>306
/note="primary transcript"
repeat_unit 347..373
/note="inverted repeat"
RBS 393..398
/note="ribosome binding site"
CDS 407..1366
/note="metapyrocatechase (AA 1-320)"
/codon_start=407
BASE COUNT 377 a 638 c 627 g 378 t
ORIGIN
1 gatatcggat tgggtgttca tggcgtcctt ccttacaggg attaccctca tggggttttc
61 cctgattgta gcaaaggaat tgtcgcgttg cagcattacg taaatgtacg tatgcgaaaa
121 cgcaacgtcg cgttggaaag aaggcaatca ccgccgcggc ccgcctgcgc tgcgcggaag
181 ccggtcacga caccggcacg atccacccag ccccgcgcag tgtgactcca tgatgctctc
241 tccgtcacac acgcgacaaa tattcaaatc ttgtttttat atatattttt ggatagtata
301 gcttttaacc aagttctgcc aacgggcaga ttcacctagc cggaagaacc ggcccgccgc
361 gtgcgggccg gttacagaaa acagaaaacc gcaggagacg acaaacatgg acactcatcg
421 cgccgatgcg agccagcgtt cgcaagcgcc cgcggctaga ccgcgccatg ccgtgcactc
481 gatcgaccat tacgcgctgg aagtgcctga cctggcagtg gccgagcgct tcctcgacgc
541 cttcgggctg accgtggcac gtacgccgga atgccttgag gtctatgccg ctgaccagcg
601 ctgctgggct cggttctacg aaggcgaacg caagcgcctc gcctacctga gcttcagttg
661 cttcgaaggc gatttcgcgg gcatccggca gcagttggcc gccagtgggg cgactcttgt
721 cgaggacccg cgctatggcg acgagtcggg agtctggttc ttcgatcccg acggtaacct
781 ggtccaggtg aagatcggcc ccaagacttc gccgagcagc aagtctccgg cgcggctgga
841 aggcgcgccc ggtggtcagc gtggcgcggt ggtgcgtagc caggtgcagc gcgtgctgcc
901 acgccgcctg tcgcacgtac tgttgttcac cccgagcgtg cagcgtgcgc tcgacttcta
961 ccgcgacgcg ctgggactgc gcctttccga ccgatccgat gacgtcattg cgttcacgca
1021 tgccccttat ggcagcgatc accatctgct tgcgctcgtg aagagctcgg ccagaggctg
1081 gcaccacgcc gcatgggacg tcgcggacgt caacgaggtc ggccagggcg ccagccagat
1141 ggcgaaggcg ggctacacgc agggctgggg tacggggcgc catgtgctcg gctcgaacta
1201 cttcttctat gtactcgacc catggggctc gttctgcgag tactcggcgg acatcgacta
1261 tatccccgcc ggacaggcct ggccggcagg cgactttgcg gcagaggact ccctgtacca
1321 gtgggggccg gacgtgcccg agtacttcgt ccgcaatacc gaggcctgag cggcaccgcc
1381 cgagcccgca ccgctaccgt acctttcaac aattcccgcc gcgcatggca tcaattccga
1441 ctggagctgg agaatgaagt tggcaacgtt tctgctggat ggccagcaac gagtgggcct
1501 ggtatccgac gaaggcgtct tcgcttgccg ccggttgcgg caaggacgtc ggcgccctta
1561 tcgacgccgg attcggcccg cagcagtggc acagcctggt ggcccaggag tgcactggac
1621 actgccgcgc tgacctggct gccgccggtc atcagcccgc gcaagatcct ctgcgtcggg
1681 ctgaactatg cggaccatac caaggagtct cgtacgaaca gcgactaccc agcgctgttc
1741 ctgccgtgcc accagcatgg tggacatgac tgcgcgatcg tgcgcccgct cgccagcgac
1801 acgttggact acgagggtga actcgcggtc gtgattggca agggtggcaa gcatatcgca
1861 cgggaagacg ccctcagcca tgtgttcggc tactccgtct tcaacgacgg ctcggtacgc
1921 gagtaccagt tcaagtcccc gcaatggacg gtcggcaaga acttcgacgc aaccggcggc
1981 tttggtccct atgtcgtgac ggcggacgaa ctgccccggg
//