GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ARG6HDNO 1485 bp ds-DNA BCT 27-MAY-1991
DEFINITION Arthrobacter oxidans 6-hydroxy-D-nicotine oxidase (6-HDNO) gene
ACCESSION X05999 M28330
KEYWORDS 6-hydroxy-D-nicotine oxidase.
SOURCE Arthrobacter oxidans DNA.
ORGANISM Arthrobacter oxidans
Prokaryota; Bacteria; Firmicutes; Irregular asporogenous rods.
REFERENCE 1 (bases 1 to 1485; 106 to 1485)
AUTHORS Mauch,L.
JOURNAL Unpublished (1987)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Brandsch,R., Hinkkanen,A.E., Mauch,L., Nagursky,H. and Decker,K.
TITLE 6-hydroxy-D-nicotine oxidase of Arthrobacter oxidans. Gene
structure of the flavoenzyme and its relationship to
6-hydroxy-L-nicotine oxidase
JOURNAL Eur. J. Biochem. 167, 315-320 (1987)
STANDARD full staff_review
COMMENT SWISS-PROT; P08159; HDNO$ARTOX.
Data kindly reviewed (2-Mar-1988) by Brandsch R.
From EMBL entry AO6HDNO; dated 04-OCT-1990.
FEATURES Location/Qualifiers
promoter 4..11
/note="pot.-43 region"
promoter 18..23
/note="pot.-35 region"
promoter 40..46
/note="pot.-10 region"
precursor_RNA 53..>52
/note="transcript"
RBS 95..99
/note="ribosome binding site"
CDS 109..1485
/note="put.oxidase (AA 1-443)"
/codon_start=109
BASE COUNT 326 a 393 c 449 g 317 t
ORIGIN
1 cgcaaaaata acccccattg acatggacag ctgtccatgt atcaataggg tgatcactct
61 ctcgaagacg agaatgctga ttgtcagcag acaaaggagt tggaattggt gtcatcaaaa
121 ctagccaccc cccttagtat tcaaggtgaa gttatttacc ctgatgacag cggatttgat
181 gccatcgcga atatttggga cggccgtcat ttgcagcggc cgtcgctaat tgcccggtgt
241 ctgagtgcgg gcgacgttgc gaagtccgtc cgctacgcat gtgacaacgg cctggagatc
301 tcggttcgat ccggtggcca caatccgaat ggctatgcga ccaacgacgg tggcatcgtc
361 ttggacctca gactcatgaa cagcatccac atcgacaccg ccgggagtcg cgcgcggatc
421 gggggtggcg taatcagtgg cgatctcgtg aaggaggcgg ccaaattcgg tctagcggca
481 gtcaccggca tgcatcctaa ggtcgggttc tgtggactag cgctcaatgg gggcgtcggc
541 ttcctcaccc ccaaatatgg tctcgccagt gacaatatct tgggagcgac tctggtgacc
601 gcaactggtg acgttatcta ctgctctgat gatgaacgcc cggaattgtt ctgggcggtt
661 agaggtgcgg gccccaactt cggtgtcgtc acagaggtcg aggtccagct ctacgagcta
721 ccgaggaaga tgctcgccgg attcattacc tgggccccct cggtcagcga actcgcaggg
781 ctcctgacat ccctccttga tgcgctcaac gaaatggcgg atcacatcta ccccagcgtt
841 ttcgtcggcg tggacgaaaa cagggcacct tccgttacgg tatgcgtggg gcacctcgga
901 gggctcgata tcgctgaacg ggacatagca cggcttcggg gtctgggtcg gacggtgtcc
961 gattcgatcg ccgtccgatc atatgacgag gtagtggctc ttaacgctga ggtgggcagt
1021 ttcgaggacg gaatgtccaa tctctggatc gaccgggaga tcgcgatgcc gaacgctcgt
1081 ttcgccgaag cgattgcagg caatcttgac aagtttgtta gtgaacccgc aagcggaggc
1141 tccgtcaaac tggagatcga gggaatgccg ttcggaaacc ccaagaggac gcccgctaga
1201 catcgcgatg cgatgggcgt gctggccctg gcggaatgga gcggggcggc tcctggaagt
1261 gaaaagtacc ccgagttggc acgtgaactg gacgccgcgc tccttcgcgc aggcgtgacg
1321 acttctgggt ttggactgct aaacaacaac tcagaggtca cagcagagat ggtcgctgaa
1381 gtttataagc cggaagttta ttgtcgtctt gcggcagtca aacgtgaata cgaccctgag
1441 aaccgttttc gtcacaacta caatatagat cccgagggat cttga
//