GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS AVINIFH2 1655 bp ds-DNA BCT 27-MAY-1991
DEFINITION Azotobacter vinelandii nifH2 gene for vanadin-dependent alternative
nitrogenase Fe-protein and gene for a small ferrodoxin-like
protein.
ACCESSION X13519
KEYWORDS nifH2 gene; nitrogen fixation; nitrogenase; nitrogenase Fe-protein;
vanadin-dependent nitrogenase.
SOURCE Azotobacter vinelandii DNA.
ORGANISM Azotobacter vinelandii
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Azotobacteraceae.
REFERENCE 1 (bases 1 to 1655)
AUTHORS Raina,R., Reddy,M.A., Ghosal,D. and Das,H.K.
TITLE Characterization of the gene for the Fe-protein of the vanadium
dependent alternative nitrogenase of Azotobacter vinelandii and
construction of a Tn-5 mutant
JOURNAL Mol. Gen. Genet. 214, 121-127 (1988)
STANDARD full automatic
COMMENT SWISS-PROT; P14939; FERV$AZOVI. SWISS-PROT; P15335; NIF2$AZOVI.
*source: library=PHC79; clone=pMP1 and pMP2;
From EMBL entry AVNIFH2; dated 30-AUG-1990.
FEATURES Location/Qualifiers
promoter 146..160
/note="pot. nif promoter sequence"
RBS 201..205
/note="pot. ribosome binding site"
CDS 214..1083
/note="nitrogenase Fe-protein (AA 1-290)"
/codon_start=214
RBS 1191..1195
/note="pot. ribosome binding site"
CDS 1203..1397
/note="ferrodoxin-like protein (AA 1-65)"
/codon_start=1203
BASE COUNT 354 a 545 c 467 g 289 t
ORIGIN
1 gtcgacggag cgcacagcat cctggcctgg cctggattta tggagtccaa taaacctgca
61 aaaattaaaa taattcacta attaaatatg tttttttgat tttatattcc aaaaaatagg
121 caatcatcga cttatcgatc cttgtggcac cgcccttgct caactctcgt cggcacaaat
181 caaaacgcca acgaatcaac ggaggttcct aagatggcat tgcgtcagtg tgcaatttac
241 ggcaagggtg gcatcggcaa gtccaccacc acccagaacc tggtcgccgc cctcgccgaa
301 gccggcaaga aagtgatgat cgtcggttgt gacccgaaag ccgactccac ccgcctgatc
361 ctgcactcca aggcccaggg caccgtcatg gaaatggccg cgtccgccgg ctgggtcgaa
421 gacctggagc tggaagacgt gctgcagatc ggcttcggcg gcgtcaagtg cgtcgaatcc
481 ggtggcccgg agccgggcgt cggctgcgcc ggccgtggcg tgatcaccgc gatcaacttc
541 ctagaagaag aaggcgccta cagcgacgac ctagacttcg tgttctatga cgtgctgggc
601 gacgtggtat gcggcggctt cgccatgccg atccgcgaga acaaggccca ggaaatctac
661 atcgtctgct ccggcgagat gatggccatg tacgccgcca acaacatcgc caagggcagc
721 gtgaaatacg cccactccgg cagcgtgcgt ctgggcggcc tgatctgcaa cagccgcaag
781 accgaccgcg aagacgagct gatcatggcc ctggccgcga agatcggcac ccagatgatc
841 cacttcgtgc cgcgcgacaa cgtcgtgcaa cacgccgaaa tccgccgcat gaccgtgatc
901 gaatacgatc cgaaggccgg acaggccgac gagtaccgtg ccctggctcg caaaatcgtc
961 gacaacaagc tgctggtcat cccgaacccg gcctccatgg aagaactcga agagctgctg
1021 atggaattcg gcatcatgga agtcgaagac gagtccgtcg tcggcaaggc cgccgccgaa
1081 ggctgattca cccagcacag cgtttgcgga ggagcgtgcg ccgcgggctt tcggaatggc
1141 ttctcgcggc cgcggcgcac gccgccctcc cttcgaacaa cccgacctca ggagctgaca
1201 ccatggccat ggccatcgac ggctacgaat gcactgtctg cggcgactgc gagccggtct
1261 gcccgaccgg ttcgatcgtc ttcagggacg atcactacgc gatcgaagcc gacagttgca
1321 acgaatgcac cgacgtgggc gagccgcgct gtctcggcgt ctgccccgtc gacttgtgca
1381 tccagccgct cgatgactga acactgaacg actccgcacc ccgttgccgg cgcaggacat
1441 tccgcgccgt cctgccgccg gacccagaac ggcgatcgct tttcctcagg ggcgatcgcc
1501 gttttacttt tccccgctcc gctagcgccc gcggacaaca gccgtccgta tccgcgccgt
1561 tcgccgtcct cgaccggcca cgatcgcggc acagctcgcc aagctacagc ccgctccaca
1621 agctgaccat cggcatccag accttcgcca agatc
//