GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS BACSACD 2350 bp ds-DNA BCT 27-MAY-1991
DEFINITION Bacillus amyloliquefaciens sacB gene for levansucrase (EC 2.4.1.10)
ACCESSION X52988
KEYWORDS levansucrase; sacB gene.
SOURCE Bacillus amyloliquefaciens DNA.
ORGANISM Bacillus amyloliquefaciens
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2350)
AUTHORS Vasantha,N.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2350)
AUTHORS Tang,L.B., Lenstra,R., Borchert,T.V. and Nagarajan,V.
TITLE Isolation and characterization of levansucrase-encoding gene from
Bacillus amyloliquefaciens
JOURNAL Gene 96, 89-93 (1990)
STANDARD full automatic
COMMENT *source: strain=ATCC 23844;
From EMBL entry BASACB; dated 21-JAN-1991.
FEATURES Location/Qualifiers
CDS 863..2278
/note="precursor (AA -29 to 443)"
/codon_start=863
CDS 863..949
/note="signal peptide (AA -29 to -1)"
/codon_start=863
CDS 950..2278
/note="mature levansucrase (AA 1-443)"
/codon_start=950
BASE COUNT 739 a 524 c 506 g 581 t
ORIGIN
1 gaattccttc aggaaaagaa cgatggctgt cttattagcg gttgcaggca catttatttt
61 ggtcacacac gggaatgtcg gcagcctgtc tatatccggt ctggctgttt tttggggcat
121 cagctcggca tttgcgctgg cgttttacac cctccagccg catcggcttt tgaagaaatg
181 gggctccgcc attattgtcg gatggggcat gctgatgcgg agccgttctc agcctgattc
241 agccgccttg gaagtttgaa ggccaatggt cgttgtccgc atatgccgcg atcgtgttta
301 tcatcatttt cggaacgctc atcgcttttt attgctattt ggaaagcctg aaatatctga
361 gtgcctctga aaccagcctc ctcgcctgtg cagagccgct gtcagcagct tttttagcgg
421 tgatctggct gcatgttccc ttcggaatat cagaatggct gggtacttta ctgattttag
481 ccaccatcgc tttattatct atcaagaaaa aataacctct cttttttaga gaggtttttc
541 cctaggcctg aagcaccctt tagtctcaat tacccataaa ttaaaaggcc ttttttcgtt
601 ttactatcat tcaaaagagg aaaatagacc agttgtcaat agaatcagag tctaatagaa
661 tgaggtcgaa aagtaaatca cgcaggattg ttactgataa agcaggcaag acctaaaatg
721 tgttaagggc aaagtgtatt ctttggcgtc atcccttaca tattttgggt ctttttttct
781 gtaacaaacc tgccatccat gaattcggga ggatcgaaac ggcagatcgc aaaaaacagt
841 acatacagaa ggagacatga acatgaacat caaaaaaatt gtaaaacaag ccacagttct
901 gacttttacg actgcacttc tggcaggagg agcgactcaa gccttcgcga aagaaaataa
961 ccaaaaagca tacaaagaaa cgtacggcgt ctctcatatt acacgccatg atatgctgca
1021 gatccctaaa cagcagcaaa acgaaaaata ccaagtgcct caattcgatc aatcaacgat
1081 taaaaatatt gagtctgcaa aaggacttga tgtgtgggac agctggccgc tgcaaaacgc
1141 tgacggaaca gtagctgaat acaacggcta tcacgttgtg tttgctcttg cgggaagccc
1201 gaaagacgct gatgacacat caatctacat gttttatcaa aaggtcggcg acaactcaat
1261 cgacagctgg aaaaacgcgg gccgtgtctt taaagacagc gataagttcg acgccaacga
1321 tccgatcctg aaagatcaga cgcaagaatg gtccggttct gcaaccttta catctgacgg
1381 aaaaatccgt ttattctaca ctgactattc cggtaaacat tacggcaaac aaagcctgac
1441 aacagcgcag gtaaatgtgt caaaatctga tgacacactc aaaatcaacg gagtggaaga
1501 tcacaaaacg atttttgacg gagacggaaa aacatatcag aacgttcagc agtttatcga
1561 tgaaggcaat tatacatccg gcgacaacca tacgctgaga gaccctcact acgttgaaga
1621 caaaggccat aaataccttg tattcgaagc caacacggga acagaaaacg gataccaagg
1681 cgaagaatct ttatttaaca aagcgtacta cggcggcggc acgaacttct tccgtaaaga
1741 aagccagaag cttcagcaga gcgctaaaaa acgcgatgct gagttagcga acggcgccct
1801 cggtatcata gagttaaata atgattacac attgaaaaaa gtaatgaagc cgctgatcac
1861 ttcaaacacg gtaactgatg aaatcgagcg cgcgaatgtt ttcaaaatga acggcaaatg
1921 gtacttgttc actgattcac gcggttcaaa aatgacgatc gatggtatta actcaaacga
1981 tatttacatg cttggttatg tatcaaactc tttaaccggc ccttacaagc cgctgaacaa
2041 aacagggctt gtgctgcaaa tgggtcttga tccaaacgat gtgacattca cttactctca
2101 cttcgcagtg ccgcaagcca aaggcaacaa tgtggttatc acaagctaca tgacaaacag
2161 aggcttcttc gaggataaaa aggcaacatt tggcccaagc ttcttaatga acatcaaagg
2221 caataaaaca tccgttgtca aaaacagcat cctggagcaa ggacagctga cagtcaacta
2281 ataacagcaa aaagaaaatg ccgatacttc attggcattt tcttttattt ctcaacaaga
2341 tggtgaattc
//