GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS BACBGC 1985 bp ds-DNA BCT 27-MAY-1991
DEFINITION Bacillus circulans BGC gene for lichenase (EC 3.2.1.73)
ACCESSION X52880
KEYWORDS BGC gene; glycosidase; hydrolase; lichenase.
SOURCE Bacillus circulans DNA.
ORGANISM Bacillus circulans
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1985)
AUTHORS Del,r.F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1985)
AUTHORS Bueno,A., Vasquez,D.A.C.R., Correa,J. and Del,r.F.
TITLE Nucleotide sequence of a 1,3-1,4-B-D-glucanase encoding gene in
Bacillus circulans WL-12
JOURNAL Nucleic Acids Res. 18, 4248-4248 (1990)
STANDARD full automatic
COMMENT *source: library=pBR322; strain=WL-12;
From EMBL entry BCBGC; dated 25-JUL-1990.
FEATURES Location/Qualifiers
promoter 49..54
/note="pot. -35 region"
promoter 72..77
/note="pot. -10 region"
RBS 223..232
/note="pot. ribosome binding site"
CDS 239..1465
/note="lichenase (AA 1-409)"
/codon_start=239
BASE COUNT 551 a 461 c 478 g 495 t
ORIGIN
1 gatccgaaca aaaacgtaaa atcatcggta acgagtttat ttacgtattt gacgaagagt
61 ccaagcagtt tgatgatttt gcattcctgg cgcaaggtac actgtacacg gatatcgtgg
121 aaagtggcac agcgacagct caaaccatca aatcccacca gcaatgtggg cggattgcct
181 gaggacatga acttcaagct gatcaaaacc catttggacc taaaaggagc gtaaatttat
241 gagaaaaaat aggggatttt cgtttagcag caaggctgtt atgatgtgtt gtctcgcttt
301 tcttctgatt ccggcgagct ttgctttcgc cgctccaaac aagccgttcc ctcagcacac
361 gacatatacc agcggctcga tcaagcctaa ccatgttact caaagtgcga tggacagctc
421 tgtgaaagcg aaatgggaca gctggaaatc ggcttatctg aaaacggcgg gcacaggcaa
481 gtattatgta aagtatcagt ccaacgggga taccgtatct gaggcacacg ggtacggaat
541 gctggcaacg gttataatgg ccggttatga cggcaatgcg cagacctatt tcgacggact
601 ttatcaatat tataaagcgc atccgagcgc caacaattcg aaattgatgg catggaaaca
661 aaacagcagc ttccagaaca ttgaaggcgc cgactcggcc acggatggag acatggacat
721 tgcttattcg ctcctgcttg cggacaagca gtggggtagc agcggcagta tcaactacct
781 tcaggccggc aaggacatca tcaatgccat catgcaaagt gacgtgaatc agtctcaatg
841 gacgctgcgt cttggcgact gggcaacaga caacacgttc aaaaatgcta cccgtccatc
901 ggactttatg ctgaatcact tgaaggcgtt ccaggcggca actggtgatg ccagatgggc
961 gaacgtgatc gacaaaacct atactattat caactcctta tacagcggct acagctcatc
1021 aaccggatta cttccagact tcgtcgttct gtcgggctcg acgtacaagc cggcttcagc
1081 cgattttctg gaaggggcaa acgacggcag ctacgattac aactcatgcc gcacaccttg
1141 gcggatcgca acggattacc tgatgaccgg tgacagccgg gcgctcaatc agctcaatca
1201 aatgaatagc tggatctcag ccaaagtaag cggtaaccct agcaacgtaa aggatggtta
1261 taaactcaac ggcacggtta caggttccgg cggcagcggc gcgttctatg caccgtttgg
1321 tgtcagcgct atgacctcgt ccgtgaacca aaactggctg aattccgtct ggaccaaaac
1381 ggccggcagc tccaatgaag gctactatga agacagcatc aagctgttct ccatgatcgt
1441 gatgtccggt aactggtgga catactaagc aacttataat ccgttgattg ctcatatgca
1501 agcaggcagg tagattagtg aagtgacccc ttaaagttag acaaatattt ttatgcaact
1561 tgtttggcat gagctcggta ttgtaccggg ctcatgcctt ttaattttga cttgatgcgt
1621 ttgtgattgt agtaatccat gtatcgttct agttcttgct tgaagtgatc tacgctttca
1681 aactcatgaa gataaagaaa ctctgacttc ataatgccaa agaagtctcc attactgcgt
1741 tatcgtaaca attccctttg cgtgacatgc tctgggtaat cgcttgtttc tttaaagcct
1801 gtcgatactg cttcatctgg tagtgccagc cttgatccga atgcaggagg agctgatctt
1861 cgctggataa gcgtttaaag gctttattta gcatttcgga gactaatgaa tacgtagggc
1921 gagaccctac tgtgtaagtg ataatttcac tgttaaacaa gtccataaca gtgataaata
1981 aagct
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