GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS BACARGCPA 1882 bp ds-DNA BCT 27-MAY-1991
DEFINITION B. subtilis argF, partial cpa genes for ornithine
carbamoyltransferase and carbamoyl phosphate synthase, respectively
(EC 2.1.3.3, EC 6.3.5.5)
ACCESSION X53360
KEYWORDS argF gene; carbamoyl phosphate synthetase; cpa gene;
ornithine carbamoyltransferase.
SOURCE Bacillus subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1882)
AUTHORS Smith,M.C.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Mountain,A., Smith,M.C. and Baumberg,S.
TITLE Nucleotide sequence of the Bacillus subtilis argF gene encoding
ornithine carbamoyltransferase
JOURNAL Nucleic Acids Res. 18, 4594-4594 (1990)
STANDARD full staff_review
COMMENT *source: strain=EMG50; developmental stage=vegetative; *source:
library=partial Sau3A in E. coli plasmid.
From EMBL entry BSARGCPA; dated 13-AUG-1990.
FEATURES Location/Qualifiers
CDS 1..486
/note="partial cpa protein (162 AA)"
/codon_start=1
RBS 462..470
/note="ribosome binding site"
CDS 477..1433
/note="argF protein (AA 1 - 319)"
/codon_start=477
terminator 1423..1458
/note="terminator inverted repeat"
BASE COUNT 580 a 404 c 469 g 429 t
ORIGIN
1 agatctaaat ccgcagttca aaatcatcat gcagttgccg tgaagttccc tgttttttct
61 tctcatgcga tccaggatgt ggatgtcaag cttggaccgg aaatgaaatc aacgggagaa
121 ggcatgtgtg tccgatatga ttctaacagc gccttgaaaa aaatctatac aagagtctgg
181 agtcaaaaag gaagcattta tttgcaaaac gttccggaag atgtgaagga gttagcagaa
241 aacgcaggtt ttacaataca tgaaggaaca tttgcctcat ggatggaaca ggaaggaaac
301 tcgctccaca ttaatctgag cggttctgaa gaagcacgca aagaacgttt ggaagccatg
361 acgcacggca tccccgtctt tacagaagaa gaaactgtgc gggcgtcttt acaaagcggt
421 tcaggccatc cgcagcctgt atctctcaaa gacctctata aaaaggaagt ggcatcatgc
481 acacagtgac gcaaaccagt ttatacggaa aagatttatt aacgttaaag gaccttagtg
541 aagaagatat aaacgccttg ctcgcagaag ccggtgagct gaaacaaaac aaaattcagc
601 ctatattcca tggcaaaacc ttggcgatga tttttgaaaa atcatcaacg cggacccgtg
661 tttcatttga agcgggcatg gcgcagctgg gcgggagcgc cctctttcta agccagaaag
721 atctgcagct ggggcgcggt gagactgtcg ctgatacggc aaaagtgctg tcaggctatg
781 tggatgccat catgatccgg acctttgaac atgaaaaggt ggaggaactt gccaaagaag
841 ctgacattcc ggtgatcaat ggacttaccg ataaatacca cccatgccag gcgctggcgg
901 atctcttgac gattaaggag ataaaaggga agcttaaagg cgtaaaagtc gcgtacatcg
961 gtgacggaaa taacgtggcg cactccttga tgatcggctg cgcgaaaatg ggctgtgata
1021 tctcgatcgc ttcaccaaag ggatatgaag tgttagatga agcagctgaa gcggcaaaga
1081 catatgcact tcaatccggc tcttctgtca cgctgacaga tgatccgatt gaagctgtaa
1141 aagacgcgga cgtgatttat tctgatgtgt ttacaagcat ggggcaggaa gcggaagagc
1201 aagaacgtct tgctgtcttt gcgccttatc aggtgaatgc ggcgctggtc agccatgcca
1261 agcctgacta tacattttta cattgtctcc ctgcgcatcg cgaggaagaa gtgactgcgg
1321 agattattga cgggccgaac tctgcagtgt tccagcaggc ggaaaaccgt cttcatgtgc
1381 aaaaagcact gctgaaggcc attttatata aaggggaatc atcaaaaaac tgctgagcca
1441 aactcagcag ttttttggat ggcaacatat tcacagccgc ttagcgcata ctacagcaaa
1501 aaggaaaagg aggaaaaaca gtgggacagc agcaccattc cggcccggac agaaatcgcc
1561 taataacggt gtctacgtag aaatcggcga gacagggagc atggttaaaa accctcaaaa
1621 ggtccatctg tcagcagggg aaatgttccc cgaaacatca aaccacaacc ggctgtggac
1681 ctacaaaaga aagccgtgac cactaaaaaa cccaaaacaa taaatgtttt gggtttttgg
1741 ctttttaatg ctggcttgca tttttgttca taggcatgat ttctgctaaa accatgatta
1801 agacatacag tacaacagca atgacagatg atgttttgaa attatacgta acgctgttca
1861 tgcttgcaac caagtagcat gc
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