GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS BACEPR 2521 bp ds-DNA BCT 27-MAY-1991
DEFINITION Bacillus subtilis epr gene for a novel serine protease
ACCESSION X53307
KEYWORDS epr gene; secreted protein; serine protease.
SOURCE Bacillus subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2521)
AUTHORS Brueckner,R.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2521)
AUTHORS Brueckner,R., Shoseyov,O. and Doi,R.H.
TITLE Multiple active forms of a novel serine protease from Bacillus
subtilis
JOURNAL Mol. Gen. Genet. 221, 486-490 (1990)
STANDARD full automatic
COMMENT *source: strain=DB204.
From EMBL entry BSEPR; dated 16-OCT-1990.
FEATURES Location/Qualifiers
RBS 446..457
/note="ribosome binding site"
CDS 461..2395
/note="prepropeptide (AA -27 to 618)"
/codon_start=461
CDS 461..541
/note="signal peptide (AA -27 to -1)"
/codon_start=461
CDS 542..2395
/note="propeptide (AA 1 - 618)"
/codon_start=542
CDS 770..2395
/note="mature peptide (AA 104 - 618)"
/codon_start=770
terminator 2401..2441
/note="put. transcription terminator"
BASE COUNT 870 a 600 c 534 g 517 t
ORIGIN
1 gttaacagga tatccgagct tatcggccca ctcgttccca aacacactcg ccatgaaatc
61 agcatacccc ggaatcggca agctcgttaa aatcaagaag acagacccga taataatcag
121 cggcatggac tggataattc cgtcacgcaa agcgctgaga tgccgctgcc cggcaatttt
181 cccggcgaca ggcattattt tttcctccat cacccgagtg aatgtgctca tcttaaaaac
241 ccccttttct cattgctttg tgaacaacaa cctccgcaat gttttcttta tcttattttg
301 aaaacgctta gaaattcatt tggaaaattt cctcttcatg cggaaaaaat ctgcattttg
361 ctaaacaacc ctgcccatga aaattttttc cttcttacta ttaatctctc tttttttctc
421 cgatatatat atcaaacatc atagaaaaag gagatgaatc atgaaaaaca tgtcttgcaa
481 acttgttgta tcagtcactc tgtttttcag ttttctcacc ataggccctc tcgctcatgc
541 gcaaaacagc agcgagaaag aggttattgt ggtttataaa aacaaggccg gaaaggaaac
601 catcctggac agtgatgctg atgttgaaca gcagtataag catcttcccg cggtagcggt
661 cacagcagac caggagacag taaaagaatt aaagcaggat cctgatattt tgtatgtaga
721 aaacaacgta tcatttaccg cagcagacag cacggatttc aaagtgctgt cagacggcac
781 tgacacctct gacaactttg agcaatggaa ccttgagccc attcaggtga aacaggcttg
841 gaaggcagga ctgacaggaa aaaatatcaa aattgccgtc attgacagcg ggatctcccc
901 ccacgatgac ctgtcgattg ccggcgggta ttcagctgtc agttatacct cttcttacaa
961 agatgataac ggccacggaa cacatgtcgc agggattatc ggagccaagc ataacggcta
1021 cggaattgac ggcatcgcac cggaagcaca aatatacgcg gttaaagcgc ttgatcagaa
1081 cggctcgggg gatcttcaaa gtcttctcca aggaattgac tggtcgatcg caaacaggat
1141 ggacatcgtc aatatgagcc ttggcacgac gtcagacagc aaaatccttc atgacgccgt
1201 gaacaaagca tatgaacaag gtgttctgct tgttgccgca agcggtaacg acggaaacgg
1261 caagccagtg aattatccgg cggcatacag cagtgtcgtt gcggtttcag caacaaacga
1321 aaagaatcag cttgcctcct tttcaacaac tggagatgaa gttgaatttt cagcaccggg
1381 gacaaacatc acaagcactt acttaaacca gtattatgca acgggaagcg gaacatccca
1441 agcgacaccg cacgccgctg ccatgtttgc cttgttaaaa cagcgtgatc ctgccgagac
1501 aaacgtccag cttcgcgagg aaatgcggaa aaacatcgtt gatcttggta ccgcaggccg
1561 cgatcagcaa tttggctacg gcttaatcca gtataaagca caggcaacag attcagcgta
1621 cgcggcagca gagcaagcgg tgaaaaaagc ggaacaaaca aaagcacaaa tcgatatcaa
1681 caaagcgcga gaactcatca gccagctgcc gaactccgac gccaaaactg ccctgcacaa
1741 aagactggat aaagtacagt catacagaaa tgtaaaagat gcgaaagaca aagtcgcaaa
1801 ggcagaaaaa tataaaacac agcaaaccgt tgacacagca caaactgcca tcaacaagct
1861 gccaaacgga acagacaaaa agaaccttca aaaacgctta gaccaagtaa aacgatacat
1921 cgcgtcaaag caagcgaaag acaaagttgc gaaagcggaa aaaagcaaaa agaaaacaga
1981 tgtggacagc gcacaatcag caattggcaa gctgcctgca agttcagaaa aaacgtccct
2041 gcagaaacgc cttaacaaag tgaagagcac caatttgaag acggcacagc aatccgtatc
2101 tgcggctgaa aagaaatcaa ctgatgcaaa tgcggcaaaa gcacaatcag ccgtcaatca
2161 gcttcaagca ggcaaggaca aaacggcatt gcaaaaacgg ttagacaaag tgaagaaaaa
2221 ggtggcggcg gctgaagcaa aaaaagtgga aactgcaaag gcaaaagtga agaaagcgga
2281 aaaagacaaa acaaagaaat caaagacatc cgctcagtct gcagtgaatc aattaaaagc
2341 atccaatgaa aaaacaaagc tgcaaaaacg gctgaacgcc gtcaaaccga aaaagtaacc
2401 aaaaaccttt aagatttgca ttccaagtct taaaggtttt tttcattcta agaacaccac
2461 acacaacctt tttcccatcc attgtacagg cttttcatac tattgctata cagccatgaa
2521 c
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