GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS BACMBSUFI 1809 bp ds-DNA BCT 27-MAY-1991
DEFINITION Bacillus subtilis hsmFI gene for methyltransferase M.BsuFI
ACCESSION X51515
KEYWORDS cytosine methyltransferase; hsmFI gene; methyltransferase.
SOURCE Bacillus subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1809)
AUTHORS Walter,J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1809)
AUTHORS Walter,J., Noyer-Weidner,M. and Trautner,T.A.
TITLE The amino acid sequence of the CCGG recognising DNA
methyltransferase M.BsuFI: Implications for the analysis of
sequence recognition by cytosine methyltransferase.
JOURNAL EMBO J. 9, 1007-1013 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17044; MTB2$BACSU.
*source: strain=ISF18; **map: map position=60 map units;
From EMBL entry BSMBSUFI; dated 29-APR-1990.
FEATURES Location/Qualifiers
promoter 243..248
/note="-35 region"
promoter 264..269
/note="-10 region"
RBS 281..286
/note="ribosome binding site"
CDS 292..1518
/note="methyltransferase M.BsuFI (AA 1-409)"
/codon_start=292
terminator 1548..1586
/note="put. rho-independent terminator"
BASE COUNT 630 a 227 c 363 g 589 t
ORIGIN
1 gtcgactgta taatagtgct gtggggttgt gttgacagat tagataactt cgtgggggtg
61 tggagatagg atggatgtgg ctttcttgag aagttgttta aattctaata tcgaggtgat
121 gttatgttaa atagaatgtt aaaagcaagt gcaaggttat ttttacgaaa ataaaaaagt
181 aagcactacg tatcaatatt gaaatatacg gacgttttcg ttttttgggg ttataattca
241 aattaatagt gctataattt ccgtataata ttaagataaa aggtgattgc aatgcgtggt
301 ggaaatcgac tcggtgcagg aaggaaggtt attcctgagt cagaaaaaaa gaaaagaaaa
361 agcgtgtata ttacagataa actttacact agaattatgg atactgacat cgaaaattgc
421 aataatttca gtcaaaaatg tatggcattg attgaattag caatggaaaa tctaaataag
481 aacaatcaag agcatagtgt gaaaaggaac aatatattga tggtgagaga taccaaatct
541 acatacaaca aaacgaataa taattttgaa aaacaaaacc gtggaataaa attgacattt
601 attgatttat tcgctggtat tggaggaatt agattaggat ttgaagacaa atatacgaaa
661 tgtgtattta gttctgaatg ggataaatat gcagctcaaa cgtatgaagc taattatggt
721 gagaagcctc atggtgatat tacaaaaatc aacgaaaatg atattccaga tcaagatgtt
781 ttattggctg gatttccttg tcagccgttt agcaatatag gcaagcgtga aggttttgct
841 catgaaagaa ggaacattat ttttgatgtt cttaggatcc taaagaaaaa gcaacctaag
901 atgtttttgt tggaaaatgt aaaagggctt ttaacaaatg ataatgggaa tacatttcga
961 gtcattttag acaatcttaa gagtctagga tattccgttt tttatgaagt tatggatgca
1021 caaaattttg ggcttccaca aagacgtgaa cgtattgtaa ttgtgggatt tcatcctgat
1081 ttaggcatta atgatttttc atttcctaaa ggtaatcctg acaataaggt tccaattaat
1141 gctattttag agcataatcc tacagggtat tcaatttcca agcgtttgca agaaagttac
1201 ttatttaaaa aggatgatgg gaagccacag attgttgatt ttagatgcac atatcaagtt
1261 aacactttag tagctagtta tcacaaaata cagcgtctta caggaacatt tgtaaaagac
1321 ggagaaacag ggcttcgatt attcagcgaa ttagaactga aacgtttgat gggtttccct
1381 gttgatttta aagttcctgt gtcaagaaca caaatgtata ggcagtttgg aaattctgtt
1441 gctgttccca tgattaaagc tgttgcagga gcaatgaaag agcgactttt gttggctgaa
1501 atgcaggtat tgaaaaaata aaaactatag ctttataact aatatagaga ctaatatcag
1561 caagtatagt ttgctgatat tagtcttttg ctttaattgt ttgaaaaacc tttaatctgt
1621 attttttgac cacgcttttt gcttggataa gtccatttaa aaggagttcc aaattgtcct
1681 tttccactat attcttcgat gtaatgttta atataatcat cacgattcca aacagcaaat
1741 ttatttgtaa atataattaa atctaccatt tgtatcttgt cattgagtaa agggctatgt
1801 aaagaattc
//