GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS BACRPOG 1930 bp ds-DNA BCT 27-MAY-1991
DEFINITION B.subtilis rpoF gene encoding minor sigma-37 factor of RNA
polymerase, complete cds.
ACCESSION M14508
KEYWORDS RNA polymerase.
SOURCE Bacillus subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1930)
AUTHORS Duncan,M.L., Kalman,S.S., Thomas,S.M. and Price,C.W.
TITLE Gene encoding the 37,000-dalton minor sigma factor of Bacillus
subtilis RNA polymerase: Isolation, nucleotide sequence,
chromosomal locus, and cryptic function
JOURNAL J. Bacteriol. 169, 771-778 (1987)
STANDARD full automatic
COMMENT SWISS-PROT; P06574; RP37$BACSU.
Draft entry and computer-readable sequence for [1] kindly provided
by C.W.Price, 18-FEB-1987. A sequence similar to a B.subtilis
ribosome binding site is located at positions 587-594. The "ttg"
initiation codon (positions 601-603) was chosen as the start for
the open reading frame, because it was optimally spaced from the
possible ribosome binding site. An in frame "atg" codon is located
at positions 607-609. A region of dyad symmetry is located at
523-572 and could form a stem-loop structure that may influence
rpoF expression.
From EMBL entry BSRPOF; dated 19-APR-1990.
FEATURES Location/Qualifiers
CDS 601..1395
/note="37 kd minor sigma factor (ttg start codon)"
/codon_start=601
BASE COUNT 626 a 380 c 472 g 452 t
ORIGIN
1 accggattgg gcgtttttgt agggaccttt aaaatggtga aaaaacaagg tggttcgctg
61 aaacttgaaa atctttctga acggctgatc cgactgtttg acattacagg cttgaaggac
121 atcattgata tttctgcaaa gtcagaaggt ggagtgcaat gaagaataat gctgattaca
181 tcgaaatgaa agtgccggcc caacctgaat atgtgggaat tataagactg acgctgtcag
241 gggtcgcaag cagaatgggc tatacgtacg atgaaattga agacttgaaa atcgcagtca
301 gtgaggcgtg cacaaatgcg gttcagcacg cttacaaaga agataaaaat ggggaagtgt
361 caatacgatt cggtgtgttt gaagaccgtt tagaggttat tgtggcggat gaaggagaca
421 gctttgactt tgatcaaaag cagcaggatc tagggccgta cacaccttcg cacacagttg
481 atcaattatc agaaggaggg ctcggtctat atttaatgga aacgctcatg gatgaagtca
541 gagtgcaaaa ccactccggc gtcaccgtag cgatgacaaa gtatttaaat ggggagcgag
601 ttgatcatga cacaaccatc aaaaactacg aaactaacta aagatgaagt cgatcggctc
661 ataagcgatt accaaacaaa gcaagatgaa caagcgcagg aaacgcttgt gcgggtgtat
721 acaaatctgg ttgacatgct tgcgaaaaaa tactcaaaag gcaaaagctt ccacgaggat
781 ctccgccagg tcggcatgat cgggctgcta ggcgcgatta agcgatacga tcctgttgtc
841 ggcaaatcgt ttgaagcttt tgcaatcccg acaatcatcg gtgaaattaa acgtttcctc
901 agagataaaa catggagcgt tcatgtgccg agacgaatta aagaactcgg tccaagaatc
961 aaaatggcgg ttgatcagct gaccactgaa acacaaagat cgccgaaagt cgaagagatt
1021 gccgaattcc tcgatgtttc tgaagaagag gttcttgaaa cgatggaaat gggcaaaagc
1081 tatcaagcct tatccgttga ccacagcatt gaagcggatt cggacggaag cactgtcacg
1141 attcttgata tcgtcggatc acaggaggac ggatatgagc gggtcaacca gcaattgatg
1201 ctgcaaagcg tgcttcatgt cctttcagac cgtgagaaac aaatcataga ccttacgtat
1261 attcaaaaca aaagccaaaa agaaactggg gacattctcg gtatatctca aatgcacgtc
1321 tcgcgcttgc aacgcaaagc tgtgaagaag ctcagagagg ccttgattga agatccctcg
1381 atggagttaa tgtaatgatc caggttgaag aaaacgagca tattcaaact ctagtatatc
1441 aactgaataa agaagggaaa tcgatttgcg gtgacagttt ttttatgaaa gctgatgata
1501 aggagttaat ttgcgcggtt gctgacggac tgggaagtgg atcacttgct aacgaatcct
1561 ctgcagccat caaagactta gtggaaaact atgcgagtga agacgtagaa agcattatcg
1621 aacgctgtaa tcaggcgatg aaaaacaaaa gaggcgctac agcatctatc ctgaaaatca
1681 attttgagca aaggcagttt acgtactgct ctgtcggaaa tgtacggttt attctgcatt
1741 ccccgtctgg tgaaagcttt tatcctctgc cgatttcggg ctatttatca ggcaagccgc
1801 aaaaatacaa aacgcacacc gccacctatg aaaagggttc aaagttcatt atacatacag
1861 atggactcaa cgtacctgat atccgctccc atttgaaaaa aggccaatcg gtagaagaaa
1921 tatcgaattc
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