GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS BACCRYID 3903 bp ds-DNA BCT 27-MAY-1991
DEFINITION Bacillus thuringiensis cryID gene for insectidal crystal protein
(Lepidoptera specific)
ACCESSION X54160
KEYWORDS cryID gene; insecticidal crystal protein; insecticidal protein.
SOURCE Bacillus thuringiensis DNA.
ORGANISM Bacillus thuringiensis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 3903)
AUTHORS Peferoen,M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3903)
AUTHORS Hofte,H., Soetaert,P., Jansens,S. and Peferoen,M.
TITLE Nucleotide sequence and deduced amino acid sequence of a new
Lepidoptera-specific crystal protein gene from Bacillus
thuringiensis
JOURNAL Nucleic Acids Res. 18, 5545-5545 (1990)
STANDARD full automatic
COMMENT *source: strain=aizawai, HD-68;
Data kindly reviewed (18-OCT-1990) by Peferoen M.
From EMBL entry BTCRYID; dated 11-DEC-1990.
FEATURES Location/Qualifiers
promoter 162..190
/note="promoter"
RBS 253..257
/note="ribosome binding site"
CDS 264..3758
/note="cryID protein"
/codon_start=264
repeat_unit 3849..3869
/note="3' inverted repeat"
BASE COUNT 1285 a 657 c 821 g 1140 t
ORIGIN
1 ggatctgttt taatataagg gatttgtgcc cttctcgtta tattctttta ttagccccaa
61 aaactagtgc aactaaatat ttttataatt acactgatta aatactttat ttttgggagt
121 aagatttatg ctgaaatgta ataaaattcg ttccattttc tgtattttct cataaaatgt
181 ttcatatgct ttaaattgta gtaaagaaaa acagtacaaa cttaaaagga ctttagtaat
241 ttaataaaaa aaggggatag tttatggaaa taaataatca aaaccaatgt gtgccttaca
301 attgtttaag taatcctaag gagataatat taggcgagga aaggctagaa acagggaata
361 ctgtagcaga catttcatta gggcttatta attttctata ttctaatttt gtaccaggag
421 gaggatttat agtaggttta ctagaattaa tatggggatt tatagggcct tcgcaatggg
481 atattttttt agctcaaatt gagcaattga ttagtcaaag aatagaagaa tttgctagga
541 atcaggcaat ttcaagattg gaggggctaa gcaatcttta taaggtctat gttagagcgt
601 ttagcgactg ggagaaagat cctactaatc ctgctttaag ggaagaaatg cgtatacaat
661 ttaatgacat gaatagtgct ctcataacgg ctattccact ttttagagtt caaaattatg
721 aagttgctct tttatctgta tatgttcaag ccgcaaactt acatttatct attttaaggg
781 atgtttcagt tttcggagaa agatggggat atgatacagc gactatcaat aatcgctata
841 gtgatctgac tagccttatt catgtttata ctaaccattg tgtggatacg tataatcagg
901 gattaaggcg tttggaaggt cgttttctta gcgattggat tgtatataat cgtttccgga
961 gacaattgac aatttcagta ttagatattg ttgcgttttt tccaaattat gatattagaa
1021 catatccaat tcaaacagct actcagctaa cgagggaagt ctatctggat ttacctttta
1081 ttaatgaaaa tctttctcct gcagcaagct atccaacctt ttcagctgct gaaagtgcta
1141 taattagaag tcctcattta gtagactttt taaatagctt taccatttat acagatagtc
1201 tggcacgtta tgcatattgg ggagggcact tggtaaattc tttccgcaca ggaaccacta
1261 ctaatttgat aagatcccct ttatatggaa gggaaggaaa tacagagcgc cccgtaacta
1321 ttaccgcatc acctagcgta ccaatattta gaacactttc atatattaca ggccttgaca
1381 attcaaatcc tgtagctgga atcgagggag tggaattcca aaatactata agtagaagta
1441 tctatcgtaa aagcggtcca atagattctt ttagtgaatt accacctcaa gatgccagcg
1501 tatctcctgc aattgggtat agtcaccgtt tatgccatgc aacattttta gaacggatta
1561 gtggaccaag aatagcaggc accgtatttt cttggacaca ccgtagtgcc agccctacta
1621 atgaagtaag tccatctaga attacacaaa ttccatgggt aaaggcgcat actcttgcat
1681 ctggtgcctc cgtcattaaa ggtcctggat ttacaggtgg agatattctg actaggaata
1741 gtatgggcga gctggggacc ttacgagtaa ccttcacagg aagattacca caaagttatt
1801 atatacgttt ccgttatgct tcggtagcaa ataggagtgg tacatttaga tattcacagc
1861 caccttcgta tggaatttca tttccaaaaa ctatggacgc aggtgaacca ctaacatctc
1921 gttcgttcgc tcatacaaca ctcttcactc caataacctt ttcacgagct caagaagaat
1981 ttgatctata catccaatcg ggtgtttata tagatcgaat tgaatttata ccggttactg
2041 caacatttga ggcagaatat gatttagaaa gagcgcaaaa ggtggtgaat gccctgttta
2101 cgtctacaaa ccaactaggg ctaaaaacag atgtgacgga ttatcatatt gatcaggtat
2161 ccaatctagt tgcgtgttta tcggatgaat tttgtctgga tgaaaagaga gaattgtccg
2221 agaaagttaa acatgcaaag cgactcagtg atgagcggaa tttacttcaa gatccaaact
2281 tcagagggat caataggcaa ccagaccgtg gctggagagg aagtacggat attactatcc
2341 aaggaggaga tgacgtattc aaagagaatt acgttacgct accgggtacc tttgatgagt
2401 gctatccaac gtatttatat caaaaaatag atgagtcgaa attaaaagcc tatacccgtt
2461 atcaattaag agggtatatc gaagatagtc aagacttaga aatctattta attcgttaca
2521 atgcaaaaca cgaaatagta aatgtaccag gtacaggaag tttatggcct ctttctgtag
2581 aaaatcaaat tggaccttgt ggagaaccga atcgatgcgc gccacacctt gaatggaatc
2641 ctgatttaca ctgttcctgc agagacgggg aaaaatgtgc acatcattct catcatttct
2701 ctttggacat tgatgttgga tgtacagact taaatgagga cttaggtgta tgggtgatat
2761 tcaagattaa gacgcaagat ggccacgcac gactagggaa tctagagttt ctcgaagaga
2821 aaccattatt aggagaagca ctagctcgtg tgaaaagagc ggagaaaaaa tggagagaca
2881 aacgcgaaac attacaattg gaaacaacta tcgtttataa agaggcaaaa gaatctgtag
2941 atgctttatt tgtaaactct caatatgata gattacaagc ggatacgaac atcgcgatga
3001 ttcatgcggc agataaacgc gttcatagaa ttcgagaagc gtatctgccg gagctgtctg
3061 tgattccggg tgtcaatgcg gctatttttg aagaattaga agagcgtatt ttcactgcat
3121 tttccctata tgatgcgaga aatattatta aaaatggcga tttcaataat ggcttattat
3181 gctggaacgt gaaagggcat gtagaggtag aagaacaaaa caatcaccgt tcagtcctgg
3241 ttatcccaga atgggaggca gaagtgtcac aagaggttcg tgtctgtcca ggtcgtggct
3301 atatccttcg tgttacagcg tacaaagagg gatatggaga aggttgcgta acgatccatg
3361 agatcgagaa caatacagac gaactgaaat tcaacaactg tgtagaagag gaagtatatc
3421 caaacaacac ggtaacgtgt attaattata ctgcgactca agaagaatat gagggtacgt
3481 acacttctcg taatcgagga tatgacgaag cctatggtaa taacccttcc gtaccagctg
3541 attatgcgtc agtctatgaa gaaaaatcgt atacagatag acgaagagag aatccttgtg
3601 aatctaacag aggatatgga gattacacac cactaccagc tggttatgta acaaaggaat
3661 tagagtactt cccagagacc gataaggtat ggattgagat tggagaaaca gaaggaacat
3721 tcatcgtgga cagcgtggaa ttactcctta tggaggaata ggaccatccg agtatagcag
3781 tttaataaat attaattaaa atagtagtct aacttccgtt ccaattaaat aagtaaatta
3841 cagttgtaaa aaaaaacgaa cattactctt caaagagcga tgtccgtttt ttatatggtg
3901 tgt
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