GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS CLOBOTAG 4292 bp ds-DNA BCT 27-MAY-1991
DEFINITION Clostridium botulinum botA gene for type A neurotoxin
ACCESSION X52066 X52088
KEYWORDS botA gene; neurotoxin; secreted protein.
SOURCE Clostridium acetobutylicum DNA.
ORGANISM Clostridium acetobutylicum
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4292)
AUTHORS Minton,N.P.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4292)
AUTHORS Thompson,D.E., Brehm,J.K., Oultram,J.D., Swinfield,T.J.,
Shone,C.C., Atkinson,T., Melling,J. and Minton,N.P.
TITLE The complete amino acid sequence of the Clostridium botulinum type
A neurotoxin, deduced by nucleotide sequence analysis of the
encoding gene.
JOURNAL Eur. J. Biochem. 189, 73-81 (1990)
STANDARD full automatic
COMMENT *source: strain=sub sp. type A, NCTC2916;
From EMBL entry CBBOTAG; dated 29-MAY-1990.
FEATURES Location/Qualifiers
misc_feature 16..64
/note="dyad symmetry"
promoter 19..24
/note="-35 region"
promoter 41..46
/note="-10 region"
RBS 63..68
/note="ribosome binding site"
CDS 77..3964
/note="type A neurotoxin (AA 1-1296)"
/codon_start=77
misc_feature 4042..4087
/note="dyad symmetry"
misc_feature 4104..4136
/note="dyad symmetry"
misc_feature 4158..4188
/note="dyad symmetry"
BASE COUNT 1738 a 423 c 668 g 1463 t
ORIGIN
1 tcaaagtatt tgtatttatg gtcatttaaa taattaataa tttaattaat tttaaatatt
61 ataagaggtg ttaaatatgc aatttgttaa taaacaattt aattataaag atcctgtaaa
121 tggtgttgat attgcttata taaaaattcc aaatgtagga caaatgcaac cagtaaaagc
181 ttttaaaatt cataataaaa tatgggttat tccagaaaga gatacattta caaatcctga
241 agaaggagat ttaaatccac caccagaagc aaaacaagtt ccagtttcat attatgattc
301 aacatattta agtacagata atgaaaaaga taattattta aagggagtta caaaattatt
361 tgagagaatt tattcaactg atcttggaag aatgttgtta acatcaatag taaggggaat
421 accattttgg ggtggaagta caatagatac agaattaaaa gttattgata ctaattgtat
481 taatgtgata caaccagatg gtagttatag atcagaagaa cttaatctag taataatagg
541 accctcagct gatattatac agtttgaatg taaaagcttt ggacatgaag ttttgaatct
601 tacgcgaaat ggttatggct ctactcaata cattagattt agcccagatt ttacatttgg
661 ttttgaggag tcacttgaag ttgatacaaa tcctctttta ggtgcaggca aatttgctac
721 agatccagca gtaacattag cacatgaact tatacatgct ggacatagat tatatggaat
781 agcaattaat ccaaataggg tttttaaagt aaatactaat gcctattatg aaatgagtgg
841 gttagaagta agctttgagg aacttagaac atttggggga catgatgcaa agtttataga
901 tagtttacag gaaaacgaat ttcgtctata ttattataat aagtttaaag atatagcaag
961 tacacttaat aaagctaaat caatagtagg tactactgct tcattacagt atatgaaaaa
1021 tgtttttaaa gagaaatatc tcctatctga agatacatct ggaaaatttt cggtagataa
1081 attaaaattt gataagttat acaaaatgtt aacagagatt tacacagagg ataattttgt
1141 taagtttttt aaagtactta acagaaaaac atatttgaat tttgataaag ccgtatttaa
1201 gataaatata gtacctaagg taaattacac aatatatgat ggatttaatt taagaaatac
1261 aaatttagca gcaaacttta atggtcaaaa tacagaaatt aataatatga attttactaa
1321 actaaaaaat tttactggat tgtttgaatt ttataagttg ctatgtgtaa gagggataat
1381 aacttctaaa actaaatcat tagataaagg atacaataag gcattaaatg atttatgtat
1441 caaagttaat aattgggact tgttttttag tccttcagaa gataatttta ctaatgatct
1501 aaataaagga gaagaaatta catctgatac taatatagaa gcagcagaag aaaatattag
1561 tttagattta atacaacaat attatttaac ctttaatttt gataatgaac ctgaaaatat
1621 ttcaatagaa aatctttcaa gtgacattat aggccaatta gaacttatgc ctaatataga
1681 aagatttcct aatggaaaaa agtatgagtt agataaatat actatgttcc attatcttcg
1741 tgctcaagaa tttgaacatg gtaaatctag gattgcttta acaaattctg ttaacgaagc
1801 attattaaat cctagtcgtg tttatacatt tttttcttca gactatgtaa agaaagttaa
1861 taaagctacg gaggcagcta tgtttttagg ctgggtagaa caattagtat atgattttac
1921 cgatgaaact agcgaagtaa gtactacgga taaaattgcg gatataacta taattattcc
1981 atatatagga cctgctttaa atataggtaa tatgttatat aaagatgatt ttgtaggtgc
2041 tttaatattt tcaggagctg ttattctgtt agaatttata ccagagattg caatacctgt
2101 attaggtact tttgcacttg tatcatatat tgcgaataag gttctaaccg ttcaaacaat
2161 agataatgct ttaagtaaaa gaaatgaaaa atgggatgag gtctataaat atatagtaac
2221 aaattggtta gcaaaggtta atacacagat tgatctaata agaaaaaaaa tgaaagaagc
2281 tttagaaaat caagcagaag caacaaaggc tataataaac tatcagtata atcaatatac
2341 tgaggaagag aaaaataata ttaattttaa tattgatgat ttaagttcga aacttaatga
2401 gtctataaat aaagctatga ttaatataaa taaatttttg aatcaatgct ctgtttcata
2461 tttaatgaat tctatgatcc cttatggtgt taaacggtta gaagattttg atgctagtct
2521 taaagatgca ttattaaagt atatatatga taatagagga actttaattg gtcaagtaga
2581 tagattaaaa gataaagtta ataatacact tagtacagat ataccttttc agctttccaa
2641 atacgtagat aatcaaagat tattatctac atttactgaa tatattaaga atattattaa
2701 tacttctata ttgaatttaa gatatgaaag taatcattta atagacttat ctaggtatgc
2761 atcaaaaata aatattggta gtaaagtaaa ttttgatcca atagataaaa atcaaattca
2821 attatttaat ttagaaagta gtaaaattga ggtaatttta aaaaatgcta ttgtatataa
2881 tagtatgtat gaaaatttta gtactagctt ttggataaga attcctaagt attttaacag
2941 tataagtcta aataatgaat atacaataat aaattgtatg gaaaataatt caggatggaa
3001 agtatcactt aattatggtg aaataatctg gactttacag gatactcagg aaataaaaca
3061 aagagtagtt tttaaataca gtcaaatgat taatatatca gattatataa acagatggat
3121 ttttgtaact atcactaata atagattaaa taactctaaa atttatataa atggaagatt
3181 aatagatcaa aaaccaattt caaatttagg taatattcat gctagtaata atataatgtt
3241 taaattagat ggttgtagag atacacatag atatatttgg ataaaatatt ttaatctttt
3301 tgataaggaa ttaaatgaaa aagaaatcaa agatttatat gataatcaat caaattcagg
3361 tattttaaaa gacttttggg gtgattattt acaatatgat aaaccatact atatgttaaa
3421 tttatatgat ccaaataaat atgtcgatgt aaataatgta ggtattagag gttatatgta
3481 tcttaaaggg cctagaggta gcgtaatgac tacaaacatt tatttaaatt caagtttgta
3541 tagggggaca aaatttatta taaaaaaata tgcttctgga aataaagata atattgttag
3601 aaataatgat cgtgtatata ttaatgtagt agttaaaaat aaagaatata ggttagctac
3661 taatgcatca caggcaggcg tagaaaaaat actaagtgca ttagaaatac ctgatgtagg
3721 aaatctaagt caagtagtag taatgaagtc aaaaaatgat caaggaataa caaataaatg
3781 caaaatgaat ttacaagata ataatgggaa tgatataggc tttataggat ttcatcagtt
3841 taataatata gctaaactag tagcaagtaa ttggtataat agacaaatag aaagatctag
3901 taggactttg ggttgctcat gggaatttat tcctgtagat gatggatggg gagaaaggcc
3961 actgtaatta atctcaaact acatgagtct gtcaagaatt ttctgtaaac atccataaaa
4021 attttaaaat taatatgttt aagaataact agatatgagt attgctatgc taatatctag
4081 ttattttaat ttattcaata ttattacagt aagaaaaaat actattttta ttgtaaatac
4141 aagtttagtg gtatatctca taaatgatac aagatatcat tataatgatt ttgcaaatta
4201 tagttttgaa taaatatatt tacagtattt ttgaaatgat aataattact tcaaattctt
4261 tagtataatt ttttaatgtc ttaattttta ca
//