GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS CLOTOXBG 7692 bp ds-DNA BCT 27-MAY-1991
DEFINITION Clostridium difficile toxB gene for toxin B
ACCESSION X53138
KEYWORDS cytotoxin; toxB gene; toxin.
SOURCE Clostridium difficile DNA.
ORGANISM Clostridium difficile
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 7692)
AUTHORS Wilkins,T.D.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Barroso,L.A., Wang,S.Z., Phelps,C.J., Johnson,J.L. and Wilkins,T.D.
TITLE Nucleotide sequence of Clostridium difficile toxin B gene
JOURNAL Nucleic Acids Res. 18, 4004-4004 (1990)
STANDARD full staff_review
COMMENT *source: strain=VPI 10463; clone=pCD19, pCD19L;
From EMBL entry CDTOXBG; dated 16-JUL-1990.
FEATURES Location/Qualifiers
RBS 387..391
/note=" ribosome binding site"
CDS 399..7496
/note=" toxin B (AA 1 - 2366)"
/codon_start=399
BASE COUNT 3049 a 766 c 1306 g 2571 t
ORIGIN
1 tctagacaag ctgttaataa ggctaaaaat agagcattta aaaaaataaa aaaagactat
61 gaaaattatt ttaacttgta attaatgagc ttaaagagat atttataata gaaatcaaat
121 tttagaatta actttattgt aaaatcaata acttaatcta agaatatctt aatttttata
181 ttttatatag aacaaagttt acatatttat ttcagacaac gtctttattc aatcgaagag
241 caaattaatc aactgagtgt cttaaattta aaatgttagg aagtgaatgt atatgaaaac
301 ctaagtagat attagtatat tttataaata gaaaggagga tatataaaag agttttagca
361 tttagatgta aaaatattat agtaaaggag aaaattttat gagtttagtt aatagaaaac
421 agttagaaaa aatggcaaat gtaagatttc gtactcaaga agatgaatat gttgcaatat
481 tggatgcttt agaagaatat cataatatgt cagagaatac tgtagtcgaa aaatatttaa
541 aattaaaaga tataaatagt ttaacagata tttatataga tacatataaa aaatctggta
601 gaaataaagc cttaaaaaaa tttaaggaat atctagttac agaagtatta gagctaaaga
661 ataataattt aactccagtt gagaaaaatt tacattttgt ttggattgga ggtcaaataa
721 atgacactgc tattaattat ataaatcaat ggaaagatgt aaatagtgat tataatgtta
781 atgtttttta tgatagtaat gcatttttga taaacacatt gaaaaaaact gtagtagaat
841 cagcaataaa tgatacactt gaatcattta gagaaaactt aaatgaccct agatttgact
901 ataataaatt cttcagaaaa cgtatggaaa taatttatga taaacagaaa aatttcataa
961 actactataa agctcaaaga gaagaaaatc ctgaacttat aattgatgat attgtaaaga
1021 catatctttc aaatgagtat tcaaaggaga tagatgaact taatacctat attgaagaat
1081 ccttaaataa aattacacag aatagtggaa atgatgttag aaactttgaa gaatttaaaa
1141 atggagagtc attcaactta tatgaacaag agttggtaga aaggtggaat ttagctgctg
1201 cttctgacat attaagaata tctgcattaa aagaaattgg tggtatgtat ttagatgttg
1261 atatgttacc aggaatacaa ccagacttat ttgagtctat agagaaacct agttcagtaa
1321 cagtggattt ttgggaaatg acaaagttag aagctataat gaaatacaaa gaatatatac
1381 cagaatatac ctcagaacat tttgacatgt tagacgaaga agttcaaagt agttttgaat
1441 ctgttctagc ttctaagtca gataaatcag aaatattctc atcacttggt gatatggagg
1501 catcaccact agaagttaaa attgcattta atagtaaggg tattataaat caagggctaa
1561 tttctgtgaa agactcatat tgtagcaatt taatagtaaa acaaatcgag aatagatata
1621 aaatattgaa taatagttta aatccagcta ttagcgagga taatgatttt aatactacaa
1681 cgaatacctt tattgatagt ataatggctg aagctaatgc agataatggt agatttatga
1741 tggaactagg aaagtattta agagttggtt tcttcccaga tgttaaaact actattaact
1801 taagtggccc tgaagcatat gcggcagctt atcaagattt attaatgttt aaagaaggca
1861 gtatgaatat ccatttgata gaagctgatt taagaaactt tgaaatctct aaaactaata
1921 tttctcaatc aactgaacaa gaaatggcta gcttatggtc atttgacgat gcaagagcta
1981 aagctcaatt tgaagaatat aaaaggaatt attttgaagg ttctcttggt gaagatgata
2041 atcttgattt ttctcaaaat atagtagttg acaaggagta tcttttagaa aaaatatctt
2101 cattagcaag aagttcagag agaggatata tacactatat tgttcagtta caaggagata
2161 aaattagtta tgaagcagca tgtaacttat ttgcaaagac tccttatgat agtgtactgt
2221 ttcagaaaaa tatagaagat tcagaaattg catattatta taatcctgga gatggtgaaa
2281 tacaagaaat agacaagtat aaaattccaa gtataatttc tgatagacct aagattaaat
2341 taacatttat tggtcatggt aaagatgaat ttaatactga tatatttgca ggttttgatg
2401 tagattcatt atccacagaa atagaagcag caatagattt agctaaagag gatatttctc
2461 ctaagtcaat agaaataaat ttattaggat gtaatatgtt tagctactct atcaacgtag
2521 aggagactta tcctggaaaa ttattactta aagttaaaga taaaatatca gaattaatgc
2581 catctataag tcaagactct attatagtaa gtgcaaatca atatgaagtt agaataaata
2641 gtgaaggaag aagagaatta ttggatcatt ctggtgaatg gataaataaa gaagaaagta
2701 ttataaagga tatttcatca aaagaatata tatcatttaa tcctaaagaa aataaaatta
2761 cagtaaaatc taaaaattta cctgagctat ctacattatt acaagaaatt agaaataatt
2821 ctaattcaag tgatattgaa ctagaagaaa aagtaatgtt aacagaatgt gagataaatg
2881 ttatttcaaa tatagatacg caaattgttg aggaaaggat tgaagaagct aagaatttaa
2941 cttctgactc tattaattat ataaaagatg aatttaaact aatagaatct atttctgatg
3001 cactatgtga cttaaaacaa cagaatgaat tagaagattc tcattttata tcttttgagg
3061 acatatcaga gactgatgag ggatttagta taagatttat taataaagaa actggagaat
3121 ctatatttgt agaaactgaa aaaacaatat tctctgaata tgctaatcat ataactgaag
3181 agatttctaa gataaaaggt actatatttg atactgtaaa tggtaagtta gtaaaaaaag
3241 taaatttaga tactacacac gaagtaaata ctttaaatgc tgcatttttt atacaatcat
3301 taatagaata taatagttct aaagaatctc ttagtaattt aagtgtagca atgaaagtcc
3361 aagtttacgc tcaattattt agtactggtt taaatactat tacagatgca gccaaagttg
3421 ttgaattagt atcaactgca ttagatgaaa ctatagactt acttcctaca ttatctgaag
3481 gattacctat aattgcaact attatagatg gtgtaagttt aggtgcagca atcaaagagc
3541 taagtgaaac gagtgaccca ttattaagac aagaaataga agctaagata ggtataatgg
3601 cagtaaattt aacaacagct acaactgcaa tcattacttc atctttgggg atagctagtg
3661 gatttagtat acttttagtt cctttagcag gaatttcagc aggtatacca agcttagtaa
3721 acaatgaact tgtacttcga gataaggcaa caaaggttgt agattatttt aaacatgttt
3781 cattagttga aactgaagga gtatttactt tattagatga taaaataatg atgccacaag
3841 atgatttagt gatatcagaa atagatttta ataataattc aatagtttta ggtaaatgtg
3901 aaatctggag aatggaaggt ggttcaggtc atactgtaac tgatgatata gatcacttct
3961 tttcagcacc atcaataaca tatagagagc cacacttatc tatatatgac gtattggaag
4021 tacaaaaaga agaacttgat ttgtcaaaag atttaatggt attacctaat gctccaaata
4081 gagtatttgc ttgggaaaca ggatggacac caggtttaag aagcttagaa aatgatggca
4141 caaaactgtt agaccgtata agagataact atgaaggtga gttttattgg agatattttg
4201 cttttatagc tgatgcttta ataacaacat taaaaccaag atatgaagat actaatataa
4261 gaataaattt agatagtaat actagaagtt ttatagttcc aataataact acagaatata
4321 taagagaaaa attatcatat tctttctatg gttcaggagg aacttatgca ttgtctcttt
4381 ctcaatataa tatgggtata aatatagaat taagtgaaag tgatgtttgg attatagatg
4441 ttgataatgt tgtgagagat gtaactatag aatctgataa aattaaaaaa ggtgatttaa
4501 tagaaggtat tttatctaca ctaagtattg aagagaataa aattatctta aatagccatg
4561 agattaattt ttctggtgag gtaaatggaa gtaatggatt tgtttcttta acattttcaa
4621 ttttagaagg aataaatgca attatagaag ttgatttatt atctaaatca tataaattac
4681 ttatttctgg cgaattaaaa atattgatgt taaattcaaa tcatattcaa cagaaaatag
4741 attatatagg attcaatagc gaattacaga aaaatatacc atatagcttt gtagatagtg
4801 aaggaaaaga gaatggtttt attaatggtt caacaaaaga aggtttattt gtatctgaat
4861 tacctgatgt agttcttata agtaaggttt atatggatga tagtaagcct tcatttggat
4921 attatagtaa taatttgaaa gatgtcaaag ttataactaa agataatgtt aatatattaa
4981 caggttatta tcttaaggat gatataaaaa tctctctttc tttgactcta caagatgaaa
5041 aaactataaa gttaaatagt gtgcatttag atgaaagtgg agtagctgag attttgaagt
5101 tcatgaatag aaaaggtaat acaaatactt cagattcttt aatgagcttt ttagaaagta
5161 tgaatataaa aagtattttc gttaatttct tacaatctaa tattaagttt atattagatg
5221 ctaattttat aataagtggt actacttcta ttggccaatt tgagtttatt tgtgatgaaa
5281 atgataatat acaaccatat ttcattaagt ttaatacact agaaactaat tatactttat
5341 atgtaggaaa tagacaaaat atgatagtgg aaccaaatta tgatttagat gattctggag
5401 atatatcttc aactgttatc aatttctctc aaaagtatct ttatggaata gacagttgtg
5461 ttaataaagt tgtaatttca ccaaatattt atacagatga aataaatata acgcctgtat
5521 atgaaacaaa taatacttat ccagaagtta ttgtattaga tgcaaattat ataaatgaaa
5581 aaataaatgt taatatcaat gatctatcta tacgatatgt atggagtaat gatggtaatg
5641 attttattct tatgtcaact agtgaagaaa ataaggtgtc acaagttaaa ataagattcg
5701 ttaatgtttt taaagataag actttggcaa ataagctatc ttttaacttt agtgataaac
5761 aagatgtacc tgtaagtgaa ataatcttat catttacacc ttcatattat gaggatggat
5821 tgattggcta tgatttgggt ctagtttctt tatataatga gaaattttat attaataact
5881 ttggaatgat ggtatctgga ttaatatata ttaatgattc attatattat tttaaaccac
5941 cagtaaataa tttgataact ggatttgtga ctgtaggcga tgataaatac tactttaatc
6001 caattaatgg tggagctgct tcaattggag agacaataat tgatgacaaa aattattatt
6061 tcaaccaaag tggagtgtta caaacaggtg tatttagtac agaagatgga tttaaatatt
6121 ttgccccagc taatacactt gatgaaaacc tagaaggaga agcaattgat tttactggaa
6181 aattaattat tgacgaaaat atttattatt ttgatgataa ttatagagga gctgtagaat
6241 ggaaagaatt agatggtgaa atgcactatt ttagcccaga aacaggtaaa gcttttaaag
6301 gtctaaatca aataggtgat tataaatact atttcaattc tgatggagtt atgcaaaaag
6361 gatttgttag tataaatgat aataaacact attttgatga ttctggtgtt atgaaagtag
6421 gttacactga aatagatggc aagcatttct actttgctga aaacggagaa atgcaaatag
6481 gagtatttaa tacagaagat ggatttaaat attttgctca tcataatgaa gatttaggaa
6541 atgaagaagg tgaagaaatc tcatattctg gtatattaaa tttcaataat aaaatttact
6601 attttgatga ttcatttaca gctgtagttg gatggaaaga tttagaggat ggttcaaagt
6661 attattttga tgaagataca gcagaagcat atataggttt gtcattaata aatgatggtc
6721 aatattattt taatgatgat ggaattatgc aagttggatt tgtcactata aatgataaag
6781 tcttctactt ctctgactct ggaattatag aatctggagt acaaaacata gatgacaatt
6841 atttctatat agatgataat ggtatagttc aaattggtgt atttgatact tcagatggat
6901 ataaatattt tgcacctgct aatactgtaa atgataatat ttacggacaa gcagttgaat
6961 atagtggttt agttagagtt ggggaagatg tatattattt tggagaaaca tatacaattg
7021 agactggatg gatatatgat atggaaaatg aaagtgataa atattatttc aatccagaaa
7081 ctaaaaaagc atgcaaaggt attaatttaa ttgatgatat aaaatattat tttgatgaga
7141 agggcataat gagaacgggt cttatatcat ttgaaaataa taattattac tttaatgaga
7201 atggtgaaat gcaatttggt tatataaata tagaagataa gatgttctat tttggtgaag
7261 atggtgtcat gcagattgga gtatttaata caccagatgg atttaaatac tttgcacatc
7321 aaaatacttt ggatgagaat tttgagggag aatcaataaa ctatactggt tggttagatt
7381 tagatgaaaa gagatattat tttacagatg aatatattgc agcaactggt tcagttatta
7441 ttgatggtga ggagtattat tttgatcctg atacagctca attagtgatt agtgaataga
7501 taaaaatatg ttaaatatat cctcttatac ttaaatatat aaaaataaac aaaatgatac
7561 actacataaa gtgttctatc taatatgaag atttaccaat aaaaaggtgg actatgatga
7621 atgcacagta gttcaccttt ttatattact aatggtaaca aaatattttt ttatataaac
7681 ctaggaggcg tt
//