GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS CLOTOXINA 1948 bp ds-DNA BCT 27-MAY-1991 DEFINITION Clostridium difficile cdtA gene for enterotoxin A, part. ACCESSION X17194 KEYWORDS enterotoxin; enterotoxin A; secreted protein. SOURCE Clostridium difficile DNA. ORGANISM Clostridium difficile Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci; Bacillaceae. REFERENCE 1 (bases 1 to 1948) AUTHORS Wren,B.W. JOURNAL Unpublished (1989) STANDARD full automatic REFERENCE 2 (sites) AUTHORS Wren,B.W., Clayton,C. and Tabaqchali,S. TITLE Rapid identification of toxigenic Clostridium dificile by polymerase chain reaction JOURNAL Lancet 335, 423-423 (1990) STANDARD full staff_review COMMENT *source: strain=clinical isolate type W1. From EMBL entry CDTOXINA; dated 19-JUN-1990. FEATURES Location/Qualifiers CDS <1..>1948 /note="enterotoxin A (1948 is 2nd base in codon)" /codon_start=1 misc_feature 1..396 /note="repeating unit" misc_feature 742..1137 /note="repeating unit" BASE COUNT 735 a 243 c 301 g 669 t ORIGIN 1 ataggggtgt ttaaaggacc taaaggattt gaatattttg cacctgctaa tacttataat 61 aataacatag aaggtcaggc tatagtttat caaagtaaat tcttaacttt gaatggtaaa 121 aaatattact ttgataataa ctcaaaagca gttaccggat ggcaaactat tgatagtaaa 181 aaatattact ttaatactaa cactgctgaa gcagctactg gatggcaaac tattgatggt 241 aaaaaatatt actttaatac taacactgct gaagcagcta ctggatggca aactattgat 301 ggtaaaaaat attactttaa tactaacact gctatagctt caactggtta tacaattatt 361 aatggtaaac atttttattt taatactgat ggtattatgc agataggagt gtttaaagga 421 cctaatggat ttgaatattt tgcacctgct aatacggatg ctaacaacat agaaggtcaa 481 gctatacttt accaaagtga attcttaact ttgaatggta aaaaatatta ttttgataat 541 aactcaaaag cagttaccgg atggcaaact attgatagta aaaaatatta ctttaatact 601 aacactgcta tagcttcaac tggttatact attattaatg gaaaacattt ttatttaaat 661 actgatggta ttatagcttc aactggttat acaattatta atggtaacca tttttatttt 721 aatactgatg gtattatgaa aataggggtt tttaaaggac ctaatggatt tgaatatttt 781 gcacctgcta atactgatca taataatata gaaggtcagg ctatagttta tcaaagtaaa 841 ttcttaactt tgaatggtaa aaaatattat tttgataata attcaaaagc agttactgga 901 tggcaaacta ttgatagtaa aaaatattac tttaatacta acactgctga agcagctact 961 ggatggcaaa ctattgatgg taaaaaatat tactttaata ctaacactgc tgaagcagct 1021 actggatggc aaactattga tggtaaaaaa tattacttta atactaacac tgctatagct 1081 tcaactggtt atacaattat taatggtaaa catttttatt ttaatactga tggtattatc 1141 ggagtgttta aaggacctaa tggatttgaa tattttgcac ctgctaatac tgatgctaat 1201 aacatagaag gtcaagctat agtttatcaa agtaaatttt taactttgaa tggtaaaaaa 1261 tattactttg ataataactc aaaagcagtt actggatggc atactattga tagtaaaaaa 1321 tattacttta atactaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa 1381 aaatattact ttaatactaa cactgctata gcttcaactg gttatacaat taataatgga 1441 aaacattttt attttaatac tgatggtatt ataggagtgt ttaaaggacc taatggattt 1501 gaatattttg cacctgctaa tacggatgct aacaacatag aaggtcaagc tatagtttat 1561 caaagtaaat tcttaacttt gaatggtaaa aaatattatt ttgataataa ctcaaatgct 1621 gttaccggat gtcaaactat tgatagtaaa aaatattact ttaatactaa cactgctata 1681 gcttcaactg gttatacaat tattaatggt aaacattttt attttaatac tgatggtatt 1741 ataggggtgt ttaaaggtcc taatggattt gaatattttg cacctgctaa tacttatgat 1801 aataacatag aaggtcaggc tatagtttat caaagtaaat tattaacttt gaatggtaaa 1861 aaatattatt ttgataataa ctcaaaagca gttaccggat ggcaaactat tgatagtaaa 1921 aaatattact ttaatactaa cactgcag //