GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOCS3P 4746 bp ds-DNA BCT 27-MAY-1991
DEFINITION Escherichia coli DNA for genes involved in synthesis of CS3 pili
ACCESSION X16944
KEYWORDS adhesion factor; fimbrial biosynthesis; pilin.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4746)
AUTHORS Manning,P.A.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4746)
AUTHORS Jalajakumari,M.B., Thomas,C.J., Halter,R. and Manning,P.A.
TITLE Genes for biosynthesis and assembly of CS3 pili of CFA/II
enterotoxigenic Escherichia coli: novel regulation of pilus
production by bypassing an amber codon
JOURNAL Mol. Microbiol. 3, 1685-1695 (1989)
STANDARD full automatic
COMMENT SWISS-PROT; P15483; CS31$ECOLI. SWISS-PROT; P15484; CS32$ECOLI.
SWISS-PROT; P15485; CS33$ECOLI. SWISS-PROT; P15486; CS34$ECOLI.
SWISS-PROT; P15487; CS35$ECOLI. SWISS-PROT; P15488; FMC3$ECOLI.
*source: strain=PB176; clone=pPM484; Amber stop codon at 3577-3579
can be read through, resulting in a 104kD protein.
From EMBL entry ECCS3P; dated 13-FEB-1990.
FEATURES Location/Qualifiers
CDS 378..1100
/note="27kD protein (AA 1 to 241)"
/codon_start=378
misc_feature 1318..4128
/note="104kD protein (AA 1 to 937)"
CDS 1858..3576
/note="63kD protein (AA 1 to 573)"
/codon_start=1858
CDS 2266..3576
/note="48kD protein (AA 1 to 437)"
/codon_start=2266
CDS 2668..3576
/note="33kD protein (AA 1 to 303)"
/codon_start=2668
CDS 3031..3576
/note="20kD protein (AA 1 to 182)"
/codon_start=3031
misc_feature 3577..3579
/note="Amber stop codon"
CDS 4153..4656
/note="precursor polypeptide (AA -22 to 146)"
/codon_start=4153
CDS 4153..4218
/note="signal peptide (AA -22 to -1)"
/codon_start=4153
CDS 4219..4656
/note="CS3 pilin (AA 1 to 146)"
/codon_start=4219
BASE COUNT 1588 a 745 c 957 g 1456 t
ORIGIN
1 aagcttcacg acatagcggg gaggtttgct tctttgagag gcgggtttac gtttacgggg
61 tttagctgaa cgggccatat aaccacctga aagacaatga catttcctgt ttttataacg
121 gtaattgcag accatgacaa gccacagccg tcaggctgtc tactcggcat tgttatctct
181 ttaaaacatt gaggtgaagc tatgctgaca caggaggtaa ttacccaatc tgaataagaa
241 ttattgggtg atctcctccc atgaaaatac gcacgcgaga agtgatatag atggaatgtt
301 gtgttttttt atcaaaatta tatttgttta tggagtatta taacaataag ttattgacgc
361 ttatgctagg agaaagaatg acacctatta agctaatttt tgcagctctg tctttatttc
421 catgcagtaa catttatgca aacaatataa ccactcagaa attcgaagct atattgggtg
481 caacaagagt aatttaccac ctagatggta atggtgaaag tctaagagtt aaaaatccgc
541 agattagtcc aattctaatt caatctaaag taatggacga gggtagtaaa gataatgcgg
601 attttattgt taccccccct ctttttagac tagatgcaaa aagagaaact gacattcgta
661 tagttatggt gaatggctta tacccaaaag acagggaatc tctaaagacc ctctgtgtgc
721 gaggaattcc accaaaacaa ggagatttat gggctaacaa tgaaaaagaa tttgttggaa
781 tgaaacttaa cgtttcaatt aacacatgta ttaaattaat attaagacca cataatcttc
841 ctaaacttga tattaattcc gaagggcaga tagaatgggg gataagggat ggtaatttag
901 tagcaaagaa taaaacacct tactatttta ctatagtaaa tgcatcgttt aatggaaagg
961 cactcaaaac accggggacg ctagggccgt atgagcaaaa actttacacg ctacctagta
1021 aaatttctgt atctggactg gtaaagtggg aaattattgg tgatctaggt gagagcagtg
1081 aaacaaagaa attcaatatt tgaagaatta aaagtgtact aaaaactgtc gagctaaact
1141 attcgtacta ttatttttat gtgattctgt taatgcagaa aaatatatat ttgagcgaga
1201 tttccttgct gattctgaaa aaattgattt aacattattg gagtcaagtg cctacccctc
1261 tggtcgttat tatgttagtt tgtatttgaa tggggaatac attacaaaag aatgatgatg
1321 tactttgacg ctggagaaag tgaggatttt tgtattcagt actctgtact acaggatata
1381 ggtgtaactg tgagtgggaa tcaggatgaa tgtgcaaatc ttgatgatga attaaactta
1441 agaaccaggt ttgattttta ctcgaaaaga atggatattt ttgtatcacc aaagtttgtt
1501 ccacgaaaaa aaaacggtct tgcgccaatt aaactttggg atgagggtga aaatgcgcta
1561 ttcacaagtt acaactttag tgaggattat taccatttta aaggtgacgc aagagatagt
1621 tattcacaat acgctaacat tcaaccacgc ttaaatatag gaccatggag aataagaact
1681 caagccatat ggaataaaaa taataacaca aaaggggagt ggagtaataa ttacctgtat
1741 gccgaaagag gcttaggaaa tataaagagt agactataca ttggggatgg atattttcca
1801 ttaaaaaact ttaattcgtt caaatttaaa ggaggggtgc taaaaactga tgagaatatg
1861 tatccctatt cagaaaaaac ttattcacca atagttaaag gctcggcaaa aactcaagca
1921 aaagttgaat tttttcagga tggtgtaaaa atttatagct caatcgtccc tccaggggat
1981 ttttctatct cagattatat tttatcaggc tcaaatagtg atctttatgt caaagttata
2041 gaggaaaatg gctcaattca ggaatttatc gttccattta cctatcctgc agttgcggtc
2101 cgggaaggat ttacctatta tgaaatcgct atgggagaga ctcagcagtc gaatgattat
2161 tttacacagt tatcatttac tcgtgggctt ccatatgact ttaccgtact tacatcttta
2221 gaatattctg gcttctacag atctcttgaa attgggttag ggaaaatgct tgggaatttg
2281 ggcgcattat cgttaatcta tggacagtca aactttagta aaagtgataa tagtaaaaat
2341 aaaaaatggg atatcagata taataaaaat attccggacc taaatacata tttgagtttt
2401 tctgctgtta gccaaactag aggggggtat tcttcactca gggatgcttt ggactatgag
2461 atcggagaat atacttttaa ctcaaaaaac tcctatacag cctcaataaa ccactcatta
2521 ggagagcttg gtagtttaaa ctttagtgga acatggcgaa actactggga gaataagaac
2581 caaaccagat cttacaattt atcatattct acacaaatct ttaatggaaa ggcctacttg
2641 tcaggaagtt tgattagaag tgaacttatg aattttaata ataagataag tgatactatt
2701 ttaaatatcg gtgttaatat tccctttggc ctttctcgtg gcattcaatc tgtaagttat
2761 aacaccagtt cagtgaaagg ggggaggagt actcatcagt tagggataag tggttctgaa
2821 tttgacaata aattgtactg gcatgtaaat cagggttact cagataatta cagtaatacc
2881 tctatgtatg gttattataa agctaagtat gctcaggtta atgccggata ctcagtttct
2941 gagagataca atcatgctta tggaggtata gagggaggaa ttctggtata tgacggtgga
3001 attattttag gtcgcaatct tggtgataca atgtcaatta ttgaagctcc aggtgcggaa
3061 aatacaaaga ttagaggatg gggatcgatt gaaactgatt ggagggggag ggcttttatt
3121 ggttatcttt caccttacca aaataatgat atatcccttg acccatcatc attaccatta
3181 gactcctctt tagatatcac aacaaattcg gttattccaa caactggtgc aattgttaaa
3241 acgacatata atgttaaaaa aggaaaaaaa gtaatgctta ctttaaaaaa gtcaaatggt
3301 gatgcagttc catttggagc aattgtgaca gttatggatg gcgatcaaaa tacaagcatt
3361 gtgggcgata atgggcaatt gtatttaggt tcctcaatgg atacaggaag gctaaaagtt
3421 atatggggaa atggcgaaga taaaaaatgt gttgttgact acatagtagg tgacaataaa
3481 aatatagcgg gtatttatat aggcagtgcc gaacatgtat ttagctcaat gctcctttat
3541 ggcaaaaaaa tatctttttt atccgcttct gtttggtagg ttataggtgt tgttaaagcg
3601 tttctgacaa ctctgcaatc caataacgaa tggagaacac acagtgaaaa aaatgatttt
3661 agcattgact ttgatgtcgg tgtggggagg tcgtttgccg cagtgggccc aacgaaagat
3721 atgagtttag gtgcaaattt aacttcagag cctacattag ctattgattt tacgcctatt
3781 gaaaatattt atgtaggtgc caattatggt aaagatattg gaacccttgt tttcacaaca
3841 aatgatttaa cagatattac attgatgtca tctcgcagcg ttgttgatgg tcgccagact
3901 ggttttttta ccttcatgga ctcatcagcc acttacaaaa ttagtacaaa actgggatca
3961 tcgaatgatg taaacattca agaaattact caaggagcta aaattactcc tgttagtgga
4021 gagaaaactt tgcctaaaaa attcactctt aagctacatg cacacaggag tagcagtaca
4081 gttccagata cgtatactgt tggtcttaac gtaaccagta atgttattta aagtgaatgt
4141 atgagggatt cgatgttaaa aataaaatac ttattaatag gtctttcact gtcagctatg
4201 agttcatact cactagctgc agcggggccc actctaacca aagaactggc attaaatgtg
4261 ctttctcctg cagctctgga tgcaacttgg gctcctcagg ataatttaac attatccaat
4321 actggcgttt ctaatacttt ggtgggtgtt ttgactcttt caaataccag tattgataca
4381 gttagcattg cgagtacaaa tgtttctgat acatctaaga atggtacagt aacttttgca
4441 catgagacaa ataactctgc tagctttgcc accaccattt caacagataa tgccaacatt
4501 acgttggata aaaatgctgg aaatacgatt gttaaaacta caaatgggag tcagttgcca
4561 actaatttac cacttaagtt tattaccact gaaggtaacg aacatttagt ttcaggtaat
4621 taccgtgcaa atataacaat tacttcgaca attaaataat tatataatag acgtagcctt
4681 cgaaataaag gctacgttgc tatctttatg tttgtgattt ataggcatca ttaaatagtc
4741 aagctt
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