GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOCYSSG 2194 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli cysS gene for cysteinyl-tRNA synthetase
ACCESSION X56234
KEYWORDS aminoacyl-transfer RNA synthetase; cysS gene;
cysteinyl-tRNA synthetase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2194)
AUTHORS Eriani,G.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Eriani,G., Dirheimer,G. and Gangloff,J.
TITLE Cysteinyl-tRNA synthetase: determination of the last E. coli
aminoacyl-tRNA synthetase primary structure
JOURNAL Nucleic Acids Res. 19, 265-269 (1991)
STANDARD full staff_review
COMMENT *source: strain=K12; **map: map position=12 min.;
From EMBL entry ECCYSSG; dated 21-JAN-1991.
FEATURES Location/Qualifiers
misc_signal 78..82
/standard_name="TTGAC box"
TATA_signal 100..103
prim_transcript 110..>2194
/gene="cysS"
mat_peptide 144..1526
/EC_number="6.1.1.16"
/product="cysteine-tRNA ligase"
/gene="cysS"
CDS 144..1529
/EC_number="6.1.1.16"
/product="cysteine-tRNA ligase"
/gene="cysS"
/codon_start=144
BASE COUNT 578 a 568 c 606 g 442 t
ORIGIN
1 tcaacccagt tcgggtcata tatagggtgg tgttatagca taaccgcacg atcggatcat
61 cacgcaatgt atgctgattc gcgcgggaaa tatgggtatt atacgcaact caattaccca
121 cacatgtcta aacggaatct tcgatgctaa aaatcttcaa tactctgaca cgccaaaaag
181 aggaatttaa gcctattcac gccggggaag tcggcatgta cgtgtgtgga atcaccgttt
241 acgatctctg tcatatcggt cacgggcgta cctttgttgc ttttgacgtg gttgcgcgct
301 atctgcgttt cctcggctat aaactgaagt atgtgcgcaa cattaccgat atcgacgaca
361 aaatcatcaa acgcgccaat gaaaatggcg aaagctttgt ggcgatggtg gatcgcatga
421 tcgccgaaat gcacaaagat tttgatgctt tgaacattct gcgcccggat atggagccgc
481 gcgcgacgca ccatatcgca gaaattattg aactcactga acaactgatc gccaaaggtc
541 acgcttatgt ggcggacaac ggcgacgtga tgttcgacgt cccgaccgat ccaacttatg
601 gcgtgctgtc gcgtcaggat ctcgaccagc tgcaggcagg cgcgcgcgtt gacgtggtcg
661 acgacaaacg caacccaatg gacttcgttc tgtggaagat gtcgaaagag ggcgaaccga
721 gctggccgtc tccgtggggc gcgggtcgtc ctggctggca cattgaatgt tcggcaatga
781 actgcaagca gctgggtaac cactttgata tccacggcgg cggttcagac ctgatgttcc
841 cgcaccacga aaacgaaatc gcgcagtcca cctgtgccca tgatggtcag tatgtgaact
901 actggatgca ctcggggatg gtgatggttg accgcgagaa gatgtccaaa tcgctgggta
961 acttctttac cgtgcgcgat gtgctgaaat actacgacgc ggaaaccgtg cgttacttcc
1021 tgatgtcggg ccactatcgc agccagttga actacagcga agagaacctg aagcaggcgc
1081 gtgcggccgt ggagcgtctc tacactgcgc tgcgcggcac agataaaacc gttgcgcctg
1141 ccggtggcga acgctttgaa gcgcgcttta ttgaagcgat ggacgacgat ttcaacaccc
1201 cggaagccta ttccgtactg tttgatatgg cgcgtgaagt aaaccgtctg aaagcagaag
1261 atatggcagc ggcgaatgca atggcatctc acctgcgtaa actttccgct gtattgggcc
1321 tgctggagca agaaccggaa gcgttcctgc aaagcggcgc gcaggcagac gacagcgaag
1381 tggctgagat tgaagcgtta attcaacagc gtctggatgc ccgtaaagcg aaagactggg
1441 cggcggcgga tgcggcgcgt gatcgtctta acgagatggg gatcgtgctg gaagatggcc
1501 cgcaagggac cacctggcgt cgtaagtaat tgcgctattg ccggatgcga gttttcgcat
1561 ccggttatcg tctgcgccac cacaacattc ccatcagtag catccccggc aaccacaccc
1621 acatcaattc agaaataatc acctgatgcc cgtacggcgt ggtgtaacga gacaatgcaa
1681 acggcgcgac ttttatcacc tgccagggag cgaaaaagcg ttcatctgac cacgccacag
1741 ccagccaacg cctttaccgc cagtggttac cgaatccagc aagctgtgcg atagcaacga
1801 gacggttaaa aacagccagc agcgaatcag cccagccctg aaccatcggc gtccaataaa
1861 cacacataac agcgggacaa caaacgcaaa caccagcgaa tgggtaaacc cgcgatgacc
1921 aaaaacattg ccgtaagcaa cgccaaattt aaacgacaat acgtcggcgt cgggcagcat
1981 cgccaggatg attccggcaa ataacagacg cggagggatg actttcgaac ccaaccctaa
2041 accaatgcat aggggaacgg cggcgtgcgt aataacggtt ggcatgatgg tcgcttcggc
2101 aaaatgtcga tgctatcagc atggatgaac ggggcgtaga gggcaaaagt ctgaaaagag
2161 aaccggcctg ttgatacagg ccgggaaagg gatc
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