GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECODHFR01 883 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli gene for type I dihydrofolate reductase (DHFR) from Tn7
ACCESSION X00926
KEYWORDS inverted repeat; reductase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 883)
AUTHORS Fling,M.E. and Richards,C.
TITLE The nucleotide sequence of the trimethoprim-resistant dihydrofolate
reductase gene harbored by Tn7
JOURNAL Nucleic Acids Res. 11, 5147-5158 (1983)
STANDARD full automatic
COMMENT SWISS-PROT; P00382; DYRB$ECOLI.
From EMBL entry ECDHFR01; dated 07-NOV-1985.
FEATURES Location/Qualifiers
promoter 183..189
/note="-35 region"
promoter 209..215
/note="-10 region"
RBS 226..229
/note="Shine-Dalgarno sequence"
CDS 236..706
/note="DHFR"
/codon_start=236
repeat_unit 728..738
/note="inverted repeat"
repeat_unit 766..776
/note="inverted repeat"
BASE COUNT 277 a 161 c 197 g 248 t
ORIGIN
1 ccggtagagt tttaatttaa tgctaaataa attaaaatgt tatgagttct ttgggtgaga
61 taatgtgcat cgtgcaagca ggatagacgg catgcacgat ttgtaataac agagtgtctt
121 gtatttttaa agaaagtcta tttaatacaa gtgattatat taattaacgg taagcatcag
181 cgggtgacaa aacgagcatg cttactaata aaatgttaac ctctgaggaa gaattgtgaa
241 actatcacta atggtagcta tatcgaagaa tggagttatc gggaatggcc ctgatattcc
301 atggagtgcc aaaggtgaac agctcctgtt taaagctatt acctataacc aatggctgtt
361 ggttggacgc aagacttttg aatcaatggg agcattaccc aaccgaaagt atgcggtcgt
421 aacacgttca agttttacat ctgacaatga gaacgtattg atctttccat caattaaaga
481 tgctttaacc aacctaaaga aaataacgga tcatgtcatt gtttcaggtg gtggggagat
541 atacaaaagc ctgatcgatc aagtagatac actacatata tctacaatag acatcgagcc
601 ggaaggtgat gtttactttc ctgaaatccc cagcaatttt aggccagttt ttacccaaga
661 cttcgcctct aacataaatt atagttacca aatctggcaa aagggttaac aagtggcagc
721 aacggattcg caaacctgtc acgccttttg tgccaaaagc cgcgccaggt ttgcgatccg
781 ctgtgccagg cgttaggcgt catatgaaga tttcggtgat ccctgagcag gtggcggaaa
841 cattggatgc tgagaaccat ttcattgttc gtgaagtgtt cga
//