GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECODUTPYR 2568 bp ds-DNA BCT 27-MAY-1991
DEFINITION Escherichia coli dut-pyrE gene region
ACCESSION V01578 X01713
KEYWORDS UTPase; orotate phosphoribosyltransferase;
unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1456 to 2568)
AUTHORS Poulsen,P., Jensen,K.F., Valentin-Hansen,P., Carlsson,P. and
Lundberg,L.G.
TITLE Nucleotide sequence of the Escherichia coli pyrE gene and of the
DNA in front of the protein-coding region
JOURNAL Eur. J. Biochem. 135, 223-229 (1983)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1609)
AUTHORS Lundberg,L.G., Thoresson,H.-O., Karlstroem,O.H. and Nyman,P.O.
TITLE Nucleotide sequence of the structural gene for dUTPase of
Escherichia coli K-12
JOURNAL EMBO J. 2, 967-971 (1983)
STANDARD full automatic
REFERENCE 3 (sites)
AUTHORS Poulsen,P., Bonekamp,F. and Jensen,K.F.
TITLE Structure of the Escherichia coli pyrE operon and control of pyrE
expression by a UTP modulated intercistronic attentuation
JOURNAL EMBO J. 3, 1783-1790 (1984)
STANDARD full staff_review
COMMENT SWISS-PROT; P00495; PYRE$ECOLI. SWISS-PROT; P03842; YPYE$ECOLI.
SWISS-PROT; P06968; DUT$ECOLI. SWISS-PROT; P17776; LPPE$ECOLI.
From EMBL entry ECDUTPYR; dated 06-JUL-1989.
FEATURES Location/Qualifiers
CDS 343..795
/note="dUTPase (dut)"
/codon_start=343
CDS 905..1537
/note="ORF"
/codon_start=905
misc_feature 1506..1529
/note="region of dyad symmetry"
CDS complement(1540..2175)
/note="orotate phosphoribosyltransferase (pyrE)"
/codon_start=2175
RBS complement(2183..2187)
/note="pot. rRNA-binding site 1"
RBS complement(2202..2205)
/note="pot. rRNA-binding site 2"
misc_feature 2214..2235
/note="region of dyad symmetry"
misc_feature 2251..2271
/note="region of dyad symmetry"
promoter complement(2279..2284)
/note="-10 region (P1)"
promoter complement(2296..2308)
/note="-35 region (P2)"
RBS complement(2406..2409)
/note="pot. rRNA-binding site 3"
promoter complement(2429..2436)
/note="-10 region (P2)"
promoter complement(2450..2461)
/note="-35 region (P1)"
BASE COUNT 613 a 714 c 631 g 610 t
ORIGIN
1 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
61 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
121 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
181 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
241 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
301 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
361 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
421 tctgccggac ttgacctgcg tgcctgtctc aacgacgccg tagaactggc tccgggtgac
481 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
541 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
601 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
661 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
721 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
781 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
841 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
901 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
961 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
1021 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
1081 cgatagcctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
1141 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
1201 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
1261 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
1321 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
1381 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
1441 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgcggcca gttgcagtaa
1501 tatgacgccg gatgactttt catccggcga gtttctttaa acgccaaact cttcgcgata
1561 ggccttaacc gccgccagat gttccgccat ttccggcttc tcttccaggt aagcaatcag
1621 gtctttcagg gtgatgatag agatcacttt gcagttgtaa tcacgctcaa cttcctgaat
1681 cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc gaaatcaaca cgccagcaag
1741 cgtcgcgcca ttggcctgaa taatctccat cgactcgcga gccgttccgg cggtgatcac
1801 atcatctacc agcattacgc gtccttgtaa cgcgctacca accagattgc cgccttcacc
1861 gtggtctttt gcttctttgc ggttaaagca gtacggcagg tccaggtcgt gatgctccgc
1921 cagtgccaca gcggttgtgg tggcaatcgg gatccctttg taagcagggc caaacagcag
1981 atcgaactca atgccggaat ccaccaacgc ttcagcgtaa aaacggccta acagtgccag
2041 atcgcgcccg gtattaaaca gcccggcgtt gaagaaatag gggcttttgc gcccggattt
2101 cagcgtaaac tcgccaaact ttaacacctg cttgctaagc gcaaattcaa taaactggcg
2161 ctgatatggt ttcatgcctt cgctcctcat cttacttttc tacagacaaa aaaaaggcga
2221 ctcatcagtc gccttaaaaa tcagtttgcc agcgccgcct tctgcgtcgc tacaatggat
2281 tcgattcccc ctcgggccag agccaacaag atgagtagct cttcatgggt gaacggctcg
2341 ccttctgccg tcccctgcac ttcaatgatg cgcccgtctt cggtcatcac tacgttcatg
2401 tcggtctctg cggcagagtc ttcaacgtat tccagatcgc aaaccgcttc gccgttcaca
2461 attccgacag aaactgcggc taccatccct ttcatcggat tggttttcag cttgccgttt
2521 tccaccagct tctgtagcgc atctaccagc gccacgcagg cacccgta
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