GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOFADAC 4950 bp ds-DNA BCT 27-MAY-1991
DEFINITION Escherichia coli fadB and fadA genes and DNA for ORF315 (EC
5.1.2.3,EC 5.3.3.8,EC 4.2.1.17,EC 1.1.1.35,EC 2.3.1.9)
ACCESSION X52837
KEYWORDS fadA gene; fadB gene; fatty acid degredation.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4950)
AUTHORS Nakahigashi,K.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1241 to 4950)
AUTHORS Nakahigashi,K. and Inokuchi,H.
TITLE Nucleotide sequence of fadB gene from Escherichia coli
JOURNAL Nucleic Acids Res. 18, 4937-4937 (1990)
STANDARD full automatic
COMMENT *source: strain=K12 W3110; **map: map position=86min; fadB and fadA
are the beta and alpha subunits of the multifunctional enzyme
complex of the fatty acid degredation cycle.
From EMBL entry ECFADAB; dated 30-AUG-1990.
FEATURES Location/Qualifiers
CDS 276..1220
/note="ORF315 (AA 1 - 315)"
/codon_start=276
promoter 1266..1271
/note="-35 region"
promoter 1289..1294
/note="-10 region"
RBS 1492..1497
/note="ribosome binding site"
CDS 1503..3689
/note="fadB gene product (AA 1 - 729)"
/codon_start=1503
RBS 3692..3696
/note="ribosome binding site"
CDS 3702..4862
/note="fadA gene product (AA 1 - 387)"
/codon_start=3702
BASE COUNT 1114 a 1319 c 1404 g 1113 t
ORIGIN
1 ccgggtcatg ttttccacgt tgttttcgtg gatcaccacg ttgtcttcga tacgaatgcc
61 gccgaacggt ttcagtgctt caattttctg ccagttgaag tgcttgctga actgcccttc
121 acgccacggt gccagtagcg attcgatgaa gtagataccc ggttcgatgg ttaacaccat
181 gcccggctgg agaatacggg tgcagcgcag gtacggatat tttgccggtg ccgcgagtgc
241 gtaccgctat catcctgcat aaaaccagcg acgtcatgca cctgcaggcc cagcggatgg
301 ccgataccat gcggcataaa cggcccggta agatcgtttt cgaccatcgc ttcttcactc
361 atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg gaactggatg
421 tggtaatcca catagctgac gcctgctttc atggtggcga tcagcgccag ttgttcatca
481 ttaacgtcct tcaccagctg cgcgtagtcg ttgtcacttt ttgccgacca ggtacgggtc
541 aggtcagccg catagccgtt atattctgcc ccggcatcca gcaggaagct gcgcatctct
601 tccggtgcct gatggtccag tttggtgtaa tgcagtaccg cagcgtgttc gttaagcgcc
661 acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcaatg
721 ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcgcg gtgaccgttg
781 accgccattt tctgcgcttc acgcatacag gccagttcgt actcagtttt gaaggagcga
841 tagtaatgca ggtagtcgat aacccctttc gggttgatgt tgctggcctc aatacccagt
901 tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc agcaggcagc
961 aggctaccaa tgccatcggc tttcggcagc gcgatcactt ccacatcttc agtccagaaa
1021 gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc
1081 ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac
1141 gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacattgaac
1201 agctcgccgg agtgaataag taacgcatcc agcttgaagc gcgccagcgc atcgcgagtc
1261 cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac
1321 ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga
1381 tttctgccga gcgtgatcag atcggcattt ctttaatctt ttgtttgcat atttttaaca
1441 caaaatacac acttcgactc atctggtacg accagatcac cttgcggatt caggagactg
1501 acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac
1561 tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg
1621 gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga
1681 acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc
1741 ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag
1801 atctgccggt gccgaccatt gctgccgtca atggctatgc gctgggcggt ggctgcgaat
1861 gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa
1921 ccaaactggg catcatgcct ggctttggcg gttctgtacg tatgccacgt atgctgggcg
1981 ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga
2041 aaatcggtct ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg
2101 ttttacgcca ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg
2161 aaccactaaa actgagcaag attgaagcca ccatgagctt caccatcgct aaagggatgg
2221 tcgcacaaac agcggggaaa cattatccgg cccccatcac cgcagtaaaa accattgaag
2281 ctgcggcccg ttttggtcgt gaagaagcct taaacctgga aaacaaaagt tttgtcccgc
2341 tggcgcatac caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa
2401 aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggcc gcggtgctgg
2461 gtgcaggcat tatgggcggc ggcatcgctt accagtctgc gtggaaaggc gtgccggttg
2521 tcatgaaaga tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc
2581 tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc gtgatctcca
2641 caatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg gtagaagcgg
2701 ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc
2761 aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc
2821 tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac cgaatgccgt
2881 tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct
2941 gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta
3001 accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt
3061 tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc ccggcatatc
3121 tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct
3181 tcccgcagcg gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc
3241 gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc
3301 cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc
3361 gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg
3421 tgcgctgtct ggaggaaggc attatcgcca ctccggcgga agcggatatg gcgctggtct
3481 acggcctggg cttgcctgcg ttccacggcg gcgcgttccg ctggctggac accctcggta
3541 gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc
3601 cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag
3661 cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg
3721 tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg
3781 cagaagatct ctccgctcat ttaatgcgta cgctgctggc gcgtaacccg gcgctggaag
3841 cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta
3901 atatcgcccg taacgcggcg ctgctggcag aagtaccaca ctctgtcccg gcggttaccg
3961 ttaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga
4021 ctggcgatgc gcaggcatgt ctggttggcg gcgtggggca tatgggccat gtgccgatga
4081 gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga
4141 tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg
4201 cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcggcc gcatttaaaa
4261 atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg
4321 acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg
4381 atccagtaaa cggtatggta acggcgggca catcttctgc actttccgat ggcgcagctg
4441 ccatgctggt gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg
4501 tgcgttcgat ggcggtcgtt ggttgtgacc catcgattat gggttacggc ccggttccgg
4561 cctcgaaact ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaaa
4621 tgaacgaagc ctttgccgcg cagatcctgc catgtattaa agatctggga ctaattgagc
4681 agattgacga gaagatcaac ctcaacggtg gcgcgatcgc gctgggtcat ccgctgggtt
4741 gttccggtgc gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt
4801 ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg
4861 tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc cggcaccaat
4921 gtaggcctga taagacgcgc cagctcgcat
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