GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOFHUE1 2900 bp ds-DNA BCT 27-MAY-1991
DEFINITION Escherichia coli fhuE gene for outer-membrane receptor for Fe(III)
-coprogen and Fe(III)-rhodotrulic acid.
ACCESSION X17615 M16186
KEYWORDS ferric coprogen receptor; ferric uptake regulation protein;
ferric-rhodotorulic receptor; fhuE gene; outer membrane protein;
receptor.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 255 to 612)
AUTHORS Sauer,M., Hantke,K. and Braun,V.
TITLE Ferric-coprogen receptor FhuE of Escherichia coli: Processing and
sequence common to all TonB-dependent outer membrane receptor
proteins
JOURNAL J. Bacteriol. 169, 2044-2049 (1987)
STANDARD unannotated staff_entry
REFERENCE 2 (sites)
AUTHORS Braun,V.
JOURNAL Unpublished (1989)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 2900)
AUTHORS Braun,V.
JOURNAL Unpublished (1989) see COMMENT for author address
STANDARD unannotated staff_entry
REFERENCE 4 (bases 1 to 2900)
AUTHORS Sauer,U., Hantke,K. and Braun,V.
TITLE Sequence of the fhuE outer-membrane receptor gene of Escherichia
coli K12 and properties of mutants
JOURNAL Mol. Microbiol. 4, 427-437 (1990)
STANDARD unannotated staff_entry
REFERENCE 5 (sites)
AUTHORS Sauer,M., Hantke,K. and Braun,V.
TITLE Sequence of the fhuE outer-membrane receptor gene of Escherichia
coli K12 and properties of mutants.
JOURNAL Mol. Microbiol. 4, 427-437 (1990)
STANDARD full staff_review
COMMENT SWISS-PROT; P16869; FHUE$ECOLI.
*source: strain=K12. **map: map position=16 min.
From EMBL entry ECFHUE1; dated 28-JUN-1990.
FEATURES Location/Qualifiers
promoter 274..279
/note="put. -35 region"
misc_signal 294..312
/note="put. fur-binding site (repressor)"
promoter 297..302
/note="put. -10 region"
misc_signal 316..334
/note="put. fur-binding site (repressor)"
RBS 395..400
/note="put. ribosome-binding site"
CDS 406..2592
/note="FhuE precursor protein"
/codon_start=406
CDS 406..513
/note="signal peptide (AA -36 to -1)"
/codon_start=406
CDS 514..2592
/note="mature FhuE protein (AA 1-693)"
/codon_start=514
misc_feature 2611..2632
/note="palindromic seq, put. transcription termination"
BASE COUNT 773 a 742 c 679 g 706 t
ORIGIN
1 ccgggcctct tgcgggatat cggaggggat ctcacgacga ataattttgc tgaatatagt
61 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc
121 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg
181 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc
241 tgcgaatgcg ctgaaaagac attctctgtt tggtttacat gacaaatatg aatgcgtata
301 tttctcattt gcatttacaa acaaaattat tcgcacacta aatataatcg ctcatcaccc
361 tcctccggca tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa
421 tttaacaggg ataatcaata tcaagccatc accaaaccgt cactacttgc cggttgcata
481 gcactggcac tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt
541 gttgagggtt cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc
601 tctgcgggta ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt
661 agccagcagc ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg
721 ctggggatca gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc
781 cagatcgata actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg
841 ggcgacgcac tttctgatat ggcactcttt gaacgcgtag aagtagtgcg tggcgcgaca
901 ggactcatga ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg
961 accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg
1021 tatgtggcgg atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc
1081 ggcggctacc agaataacga ctcatggctg gaccgctaca acagtgaaaa gaccttcttc
1141 tcgggcattg tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat
1201 cagcgcattg atgttaatag ccctacctgg ggcggtttac cgcgctggaa tactgatggc
1261 agcagcaaca gttacgatcg cgcacgcagt accgcacctg actgggcgta caacgataaa
1321 gagatcaaca aggtctttat gaccctgaag cagcagtttg ctgatacctg gcaagcgaca
1381 ctgaatgcca cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat
1441 gtaaacaaag cggatggtat gctggttggg ccatacagta attatggacc ttgctttgat
1501 tatgtcggcg gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc
1561 gctgacggta gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac
1621 agcaaacaaa acaatcgtta cttcagttca tgggccaaca tcttcccgga tgaaattggc
1681 agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg
1741 caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat
1801 ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac
1861 agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac
1921 tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca
1981 ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg
2041 atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct
2101 cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat
2161 gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag
2221 ctgacatttg gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct
2281 aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag
2341 ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacgccg
2401 tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac
2461 caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac
2521 gataccaacg tggaaggttc tatcgtctac ggcacaccgc gtaatttcag cattaccggc
2581 acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc
2641 caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg
2701 gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc
2761 agtttcttca ggccggcctg atccacttta gacacatcag caacgctgac gcgcagacgg
2821 gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc
2881 ggtgccattt cgctggtacc
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