GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOFHUE1 2900 bp ds-DNA BCT 27-MAY-1991 DEFINITION Escherichia coli fhuE gene for outer-membrane receptor for Fe(III) -coprogen and Fe(III)-rhodotrulic acid. ACCESSION X17615 M16186 KEYWORDS ferric coprogen receptor; ferric uptake regulation protein; ferric-rhodotorulic receptor; fhuE gene; outer membrane protein; receptor. SOURCE Escherichia coli DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 255 to 612) AUTHORS Sauer,M., Hantke,K. and Braun,V. TITLE Ferric-coprogen receptor FhuE of Escherichia coli: Processing and sequence common to all TonB-dependent outer membrane receptor proteins JOURNAL J. Bacteriol. 169, 2044-2049 (1987) STANDARD unannotated staff_entry REFERENCE 2 (sites) AUTHORS Braun,V. JOURNAL Unpublished (1989) STANDARD full staff_review REFERENCE 3 (bases 1 to 2900) AUTHORS Braun,V. JOURNAL Unpublished (1989) see COMMENT for author address STANDARD unannotated staff_entry REFERENCE 4 (bases 1 to 2900) AUTHORS Sauer,U., Hantke,K. and Braun,V. TITLE Sequence of the fhuE outer-membrane receptor gene of Escherichia coli K12 and properties of mutants JOURNAL Mol. Microbiol. 4, 427-437 (1990) STANDARD unannotated staff_entry REFERENCE 5 (sites) AUTHORS Sauer,M., Hantke,K. and Braun,V. TITLE Sequence of the fhuE outer-membrane receptor gene of Escherichia coli K12 and properties of mutants. JOURNAL Mol. Microbiol. 4, 427-437 (1990) STANDARD full staff_review COMMENT SWISS-PROT; P16869; FHUE$ECOLI. *source: strain=K12. **map: map position=16 min. From EMBL entry ECFHUE1; dated 28-JUN-1990. FEATURES Location/Qualifiers promoter 274..279 /note="put. -35 region" misc_signal 294..312 /note="put. fur-binding site (repressor)" promoter 297..302 /note="put. -10 region" misc_signal 316..334 /note="put. fur-binding site (repressor)" RBS 395..400 /note="put. ribosome-binding site" CDS 406..2592 /note="FhuE precursor protein" /codon_start=406 CDS 406..513 /note="signal peptide (AA -36 to -1)" /codon_start=406 CDS 514..2592 /note="mature FhuE protein (AA 1-693)" /codon_start=514 misc_feature 2611..2632 /note="palindromic seq, put. transcription termination" BASE COUNT 773 a 742 c 679 g 706 t ORIGIN 1 ccgggcctct tgcgggatat cggaggggat ctcacgacga ataattttgc tgaatatagt 61 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc 121 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg 181 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc 241 tgcgaatgcg ctgaaaagac attctctgtt tggtttacat gacaaatatg aatgcgtata 301 tttctcattt gcatttacaa acaaaattat tcgcacacta aatataatcg ctcatcaccc 361 tcctccggca tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa 421 tttaacaggg ataatcaata tcaagccatc accaaaccgt cactacttgc cggttgcata 481 gcactggcac tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt 541 gttgagggtt cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc 601 tctgcgggta ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt 661 agccagcagc ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg 721 ctggggatca gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc 781 cagatcgata actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg 841 ggcgacgcac tttctgatat ggcactcttt gaacgcgtag aagtagtgcg tggcgcgaca 901 ggactcatga ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg 961 accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg 1021 tatgtggcgg atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc 1081 ggcggctacc agaataacga ctcatggctg gaccgctaca acagtgaaaa gaccttcttc 1141 tcgggcattg tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat 1201 cagcgcattg atgttaatag ccctacctgg ggcggtttac cgcgctggaa tactgatggc 1261 agcagcaaca gttacgatcg cgcacgcagt accgcacctg actgggcgta caacgataaa 1321 gagatcaaca aggtctttat gaccctgaag cagcagtttg ctgatacctg gcaagcgaca 1381 ctgaatgcca cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat 1441 gtaaacaaag cggatggtat gctggttggg ccatacagta attatggacc ttgctttgat 1501 tatgtcggcg gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc 1561 gctgacggta gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac 1621 agcaaacaaa acaatcgtta cttcagttca tgggccaaca tcttcccgga tgaaattggc 1681 agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg 1741 caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat 1801 ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac 1861 agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac 1921 tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca 1981 ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg 2041 atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct 2101 cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat 2161 gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag 2221 ctgacatttg gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct 2281 aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag 2341 ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacgccg 2401 tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac 2461 caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac 2521 gataccaacg tggaaggttc tatcgtctac ggcacaccgc gtaatttcag cattaccggc 2581 acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc 2641 caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg 2701 gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc 2761 agtttcttca ggccggcctg atccacttta gacacatcag caacgctgac gcgcagacgg 2821 gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc 2881 ggtgccattt cgctggtacc //