GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOGUAB 1847 bp ds-DNA BCT 27-MAY-1991
DEFINITION E.coli guaB gene for IMP dehydrogenase subunit (EC 1.2.1.14)
ACCESSION X02209
KEYWORDS dehydrogenase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1847)
AUTHORS Tiedeman,A.A. and Smith,J.M.
TITLE Nucleotide sequence of the guaB locus encoding IMP dehydrogenase of
Eschericia coli K12
JOURNAL Nucleic Acids Res. 13, 1303-1316 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P06981; IMP$ECOLI.
From EMBL entry ECGUAB; dated 19-APR-1990.
FEATURES Location/Qualifiers
promoter 11..16
/note="put. -35 region"
promoter 42..42
/note="put. -10 region"
promoter 54..54
/note="put. mRNA start region"
RBS 233..235
/note="put. SD-sequence"
CDS 241..1773
/note="IMP dehydrogenase subunit"
/codon_start=241
misc_feature 273..287
/note="secondary lambda att site"
misc_feature 1777..1844
/note="guaB-guaA intercistronic region"
misc_signal 1783..1805
/note="stem-loop structure pot. transcription termination"
promoter 1808..1813
/note="pot. -10 region guaA secondry promoter"
promoter 1810..1815
/note="pot. -10 region guaA secondary promoter"
CDS 1845..1847
/note="guaA polypeptide start"
/codon_start=1845
BASE COUNT 441 a 464 c 511 g 431 t
ORIGIN
1 taaagtacca gtgaccggaa gctggttgcg tgaaattaga aatttcgccg ctgatccaaa
61 cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga ctaacggtaa
121 aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa tcagcaggtt
181 attcagtcga tagtaacccg cccttcgggg atagcaagca ttttttgcaa aaaggggtag
241 atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac tctggtcgag
301 atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt tctcctcgtt
361 cctgctcact ctaccgttct gccgaatact gctgacctca gcacccagct gacgaaaact
421 attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga agcgcgcctg
481 gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat gtccattgaa
541 cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt gactgatccg
601 cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga gcgtaacggt
661 tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat caccggtcgt
721 gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac gccgaaagag
781 cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa aatgcacgaa
841 aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg catgatcacc
901 gtgaaagact tccagaaagc ggaagctaaa ccgaacgcct gtaaagacga gcaaggccgt
961 ctgcgtgttg gtgcagcggt tggcgcaggt gcgggtaacg aagagcgtgt tgacgcgctg
1021 gttgccgcag gcgttgacgt tctgctgatc gactcctccc acggtcactc agaaggtgta
1081 ctgcaacgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaattat cggcggcaac
1141 gtggcaacag ctgcaggtgc acgcgctctg gcagaagctg gttgcagtgc ggttaaagtc
1201 ggcattggcc ctggctctat ctgtacaact cgtatcgtga ctggcgtcgg tgttccgcag
1261 attaccgctg ttgctgacgc agtagaagcc ctggaaggca ccggtattcc ggttatcgct
1321 gatggcggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg cgcaagcgcg
1381 gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat cgaactctac
1441 cagggccgtt cttacaaatc ttaccgtggt atgggttccc tgggcgcgat gtccaaaggt
1501 tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc ggaaggtatc
1561 gaaggtcgcg tagcctataa aggtcgcctg aaagagatca ttcaccagca gatgggcggc
1621 ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg tactaaagcg
1681 gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga cgtgaccatt
1741 actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga cttcatgtcg
1801 ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatg
//