GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOGUTMR 1800 bp ds-DNA BCT 27-MAY-1991
DEFINITION Escherichia coli gutM gene and gutR gene for activator and
repressor proteins
ACCESSION X13463
KEYWORDS activator protein; glucitol operon; gut operon; gutM gene;
gutR gene; repressor protein.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 553)
AUTHORS Yamada,M. and Saier,M.H.Jr..
TITLE Glucitol-specific enzymes of the phosphotransferase system in
Escherichia coli: I. Nucleotide sequence of the gut operon
JOURNAL J. Biol. Chem. 262, 5455-5463 (1987)
STANDARD full automatic
REFERENCE 2 (bases 554 to 1800)
AUTHORS Yamada,M. and Saier,M.H.Jr..
TITLE Positive and negative regulators for glucitol (gut) operon
expression in Escherichia coli
JOURNAL J. Mol. Biol. 203, 569-583 (1988)
STANDARD full automatic
COMMENT SWISS-PROT; P15081; GUTM$ECOLI. SWISS-PROT; P15082; GUTR$ECOLI.
*source: clone=pMYS24; See <J02708> for gutB and gutD genes 5' of
this sequence.
From EMBL entry ECGUTMR; dated 18-AUG-1990.
FEATURES Location/Qualifiers
RBS 341..346
/note="ribosome binding site"
CDS 353..709
/note="gut M protein (AA 1-119)"
/codon_start=353
promoter 557..562
/note="-35 region"
promoter 579..584
/note="-10 region"
precursor_RNA 592..>591
/note="primary transcript"
RBS 763..767
/note="ribosome binding site"
CDS 779..1549
/note="gut R protein (AA 1-257)"
/codon_start=779
terminator 1560..1577
/note="pot.stem loop structure"
RBS 1569..1574
/note="ribosome binding site"
terminator 1570..1582
/note="pot.stem loop structure"
misc_feature 1584..1586
/note="initiation codon gut Q protein"
BASE COUNT 454 a 456 c 460 g 430 t
ORIGIN
1 ggtgcattca ctgatgctcg gtaacctgct gaaatcgccg atgttccagt cactgttgcc
61 acaatacgcg accaagctgg gtatcaaacc ggatcaagtc gagcagtatt acatcgacaa
121 agtaccgctc aaacgcggct gcgattatca agatgtgctg aatatgctgc tgttctacgc
181 cagtcctaag gcgtcgtact gcaccggaca gtcgatcaat gtcaccggcg gtcaggtgat
241 gttctgatca acagcggaga tccattaagg atctccgctg agactataga atgcctgatg
301 cgctacgctc atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc
361 cgcactcatc accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca
421 aatttctcgt tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg
481 ccgttccagc gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca
541 gcgcatcgtc gacaccttgt ttatgaaagg actgaccgtc ttcgcccgac cgcaaaaaat
601 tcccgcaatt accggtatgc atgcgggtga tttacagccc gatgtgatct ttccccatga
661 tccactatca cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg
721 tgaatgttac ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat
781 gaaacctcgt cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc
841 ggttgaagaa ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt
901 cattctggaa catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga
961 ggaatccgat ccgcctatcg atcataaaac actcatcaac acccacaaga aagagctgat
1021 tgcagaagct gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag
1081 taccgttttg cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa
1141 cagcctgcat atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc
1201 aggcggaacg tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt
1261 cgagcatttc accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg
1321 cgtaaccacc tttaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga
1381 agtgattttg atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag
1441 tcttgaaagc gtcgataagc tgattaccga cgcaggtatc gatccggcgt ttcgtcaggc
1501 gctggaagag aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac
1561 tgaacgcggg acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac
1621 gtctgggcga tgattttgtt cgcgccgcca atatcatcct gcactgtgaa ggcaaagtgg
1681 tggtttcggg aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acgcttgcca
1741 gtaccggcac tccggctttt tttgtccatc cggcagaagc gctgcacggc gatctgggga
//