GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOILVX 2343 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli genes ilvL, ilvG and ilvE'. ilvG is acetolactate synthetase
II (E.C.4.1.3.18) ilvE is branched-chain-amino-acid
aminotransferase (E.C.2.6.1.42)
ACCESSION V00290
KEYWORDS synthetase; transferase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2343)
AUTHORS Lawther,R.P., Calhoun,D.H., Adams,C.W., Hauser,C.A., Gray,J. and
Hatfield,G.W.
TITLE Molecular basis of valine resistance in Escherichia coli K-12
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78, 922-925 (1981)
STANDARD full automatic
COMMENT SWISS-PROT; P00892; ILVG$ECOLI.
The insertion mutation (ilv02096) at base 1299 causes a frame shift
error which elongates the ilvG reading frame.
From EMBL entry ECILVX; dated 06-JUL-1989.
FEATURES Location/Qualifiers
CDS 80..178
/note="reading frame (ilvL)"
/codon_start=80
CDS 399..1301
/note="reading frame (ilvG)"
/codon_start=399
mutation 1299..1299
/note="T can be TAT"
CDS 2252..>2343
/note="reading frame (ilvE')"
/codon_start=2252
BASE COUNT 583 a 594 c 630 g 536 t
ORIGIN
1 aaaaaatatc ttgtactatt tacaaaacct atggtaactc tttaggcatt ccttcgaaca
61 agatgcaaga aaagacaaaa tgacagccct tctacgagtg attagcctgg tcgtgattag
121 cgtggtggtg attattatcc caccgtgcgg ggctgcactt ggacgaggaa aggcttagag
181 atcaagcctt aacgaactaa gacccccgca ccgaaaggtc cgggggtttt ttttgacctt
241 aaaaacataa ccgaggagca gacaatgaat aacagcacaa aattctgttt ctcaagattc
301 aggacgggga actaactatg aatggcgcac agtgggtggt acatgcgttg cgggcacagg
361 gtgtgaacac cgttttcggt tatccgggtg gcgcaattat gccggtttac gatgcattgt
421 atgacggcgg cgtggagcac ttgctatgcc gacatgagca gggtgcggca atggcggcta
481 tcggttatgc tcgtgctacc ggcaaaactg gcgtatgtat cgccacgtct ggtccgggcg
541 caaccaacct gataaccggg cttgcggacg cactgttaga ttccatccct gttgttgcca
601 tcaccggtca agtgtccgca ccgtttatcg gcactgacgc atttcaggaa gtggatgtcc
661 tgggattgtc gttagcctgt accaagcata gctttctggt gcagtcgctg gaagagttgc
721 cgcgcatcat ggctgaagca ttcgacgttg cctgctcagg tcgtcctggt ccggttctgg
781 tcgatatccc aaaagatatc cagttagcca gcggtgacct ggaaccgtgg ttcaccaccg
841 ttgaaaacga agtgactttc ccacatgccg aagttgagca agcgcgccag atgctggcaa
901 aagcgcaaaa accgatgctg tacgttggcg gtggcgtggg tatggcgcag gcagttccgg
961 ctttgcgtga atttctcgct gccacaaaaa tgcctgccac ctgtacgctg aaagggctgg
1021 gcgcagtaga agcagattat ccgtactatc tgggcatgct ggggatgcac ggcaccaaag
1081 cggcaaactt cgcggtgcag gagtgtgacc tgctgatcgc cgtgggcgca cgttttgatg
1141 accgggtgac cggcaaactg aacaccttcg cgccacacgc cagtgttatc catatggata
1201 tcgacccggc agaaatgaac aagctgcgtc aggcacatgt ggcattacaa ggtgatttaa
1261 atgctctgtt accagcatta cagcagccgt taaatcaatg actggcagca acactgcgcg
1321 cagctgcgtg atgaacattc ctggcgttac gaccatcccg gtgacgctat ctacgcgccg
1381 ttgttgttaa aacaactgtc ggatcgtaaa cctgcggatt gcgtcgtgac cacagatgtg
1441 gggcagcacc agatgtgggc tgcgcagcac atcgcccaca ctcgcccgga aaatttcatc
1501 acctccagcg gtttaggtac catgggtttt ggtttaccgg cggcggttgg cgcacaagtc
1561 gcgcgaccga acgataccgt tgtctgtatc tccggtgacg gctctttcat gatgaatgtg
1621 caagagctgg gcaccgtaaa acgcaagcag ttaccgttga aaatcgtctt actcgataac
1681 caacggttag ggatggttcg acaatggcag caactgtttt ttcaggaacg atacagcgaa
1741 accaccctta ctgataaccc cgatttcctc atgttagcca gcgccttcgg catccatggc
1801 caacacatca cccggaaaga ccaggttgaa gcggcactcg acaccatgct gaacagtgat
1861 gggccatacc tgcttcatgt ctcaatcgac gaacttgaga acgtctggcc gctggtgccg
1921 cctggcgcca gtaattcaga aatgttggag aaattatcat gatgcaacat caggtcaatg
1981 tatcggctcg cttcaatcca gaaaccttag aacgtgtttt acgcgtggtg cgtcatcgtg
2041 gtttccacgt ctgctcaatg aatatggccg ccgccagcga tgcacaaaat ataaatatcg
2101 aattgaccgt tgccagccca cggtcggtcg acttactgtt tagtcagtta aataaactgg
2161 tggacgtcgc acacgttgcc atctgccaga gcacaaccac atcacaacaa atccgcgcct
2221 gagcgcaaaa ggaatataaa aatgaccacg aagaaagctg attacatttg gttcaatggg
2281 gagatggttc gctgggaaga cgcgaaggtg catgtgatgt cgcacgcgct gcactatggc
2341 act
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