GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOIS1B 1001 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli DNA with is1B and is30B insertion sequences
ACCESSION X17345
KEYWORDS insertion sequence is1B; insertion sequence is30B.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1001; 23 to 1001)
AUTHORS Umeda,M.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 23 to 1001)
AUTHORS Umeda,M. and Ohtsubo,E.
TITLE Mapping of insertion element IS30 in the Escherichia coli K-12
chromosome
JOURNAL Mol. Gen. Genet. 222, 317-322 (1990)
STANDARD full automatic
COMMENT *source: strain=K12 W3110
From EMBL entry ECIS1B; dated 18-OCT-1990.
FEATURES Location/Qualifiers
insertion_seq 43..810
/note="is1B"
repeat_unit 43..59
/note="inverted repeat"
misc_feature complement(58..755)
/note="insA-B'-insB frame"
CDS complement(480..755)
/note="insA peptide (AA 1-91)"
/codon_start=755
repeat_unit 794..810
/note="inverted repeat"
insertion_seq 811..991
/note="is30B"
BASE COUNT 256 a 263 c 247 g 235 t
ORIGIN
1 aggctgagtg aggcttcatc gagcgattta tcgatttggc ccggtaatga ctccaactta
61 ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgctgct ccaccgattt
121 tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg
181 ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga
241 ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct
301 cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt
361 ccggagcctg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacgtatcc
421 ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt
481 taccgactgc ggcctgagtt ttttaaatgg cggaaaatcg tgttgaggcc aacgcccata
541 atgcgggcgg ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc
601 gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag
661 cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacccc gtcagtagct
721 gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat
781 acactaaatc agtaagttgg cagcatcacc gagaacacaa atgggctaat tcggcagtac
841 tttcctaaaa agacatgtct tgcccaatat actcaacatg aactagatct ggttgctgct
901 cagctaaaca acagaccgag aaagacactg aagttcaaaa caccgaaaga gataattgaa
961 aggggtgttg cattgacaga ttgaatctac agcagctttt t
//