GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOLACZ 3078 bp ds-DNA BCT 27-MAY-1991 DEFINITION E. coli gene lacZ coding for beta-balactosidase (EC 3.2.1.23). ACCESSION V00296 KEYWORDS galactosidase. SOURCE Escherichia coli DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 3078) AUTHORS Kalnins,A., Otto,K., Ruether,U. and Mueller-Hill,B. TITLE Sequence of the lacZ gene of Escherichia coli JOURNAL EMBO J. 2, 593-597 (1983) STANDARD full automatic REFERENCE 2 (sites) AUTHORS Zell,R. and Fritz,H.J. TITLE DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues JOURNAL EMBO J. 6, 1809-1815 (1987) STANDARD full staff_review COMMENT SWISS-PROT; P00722; BGAL$ECOLI. Data kindly reviewed (18-MAY-1983) by U. Ruether From EMBL entry ECLACZ; dated 06-JUL-1989. FEATURES Location/Qualifiers CDS 1..3069 /note="galactosidase" /codon_start=1 BASE COUNT 682 a 841 c 886 g 669 t ORIGIN 1 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 61 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 121 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt 181 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag 241 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac 301 accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg 361 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga 421 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt 481 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga 541 gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag 601 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca 661 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg 721 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta 781 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat 841 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg 901 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc 961 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt 1021 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat 1081 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag 1141 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg 1201 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg 1261 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta 1321 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat 1381 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct 1441 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc 1501 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc 1561 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac 1621 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc 1681 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa 1741 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag 1801 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca 1861 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc 1921 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt 1981 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt 2041 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta 2101 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg 2161 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg 2221 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc 2281 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg 2341 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc 2401 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca 2461 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac 2521 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt 2581 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg 2641 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta 2701 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg 2761 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg 2821 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc 2881 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa 2941 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc 3001 ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg 3061 tgtcaaaaat aataataa //