GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOPAP 633 bp ds-DNA BCT 27-MAY-1991
DEFINITION Eschericia coli papJ gene for PapJ protein
ACCESSION X51704
KEYWORDS PapJ protein; papJ gene; periplasmic protein.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 633)
AUTHORS Tennent,J.M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 633)
AUTHORS Tennent,J.M., Lindberg,F. and Normark,S.
TITLE Integrity of E coli P Pili During Biogenesis: properies and role of
PapJ
JOURNAL Mol. Microbiol. 4, 747-758 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17543; PAPJ$ECOLI.
*source: library=pPAP5;
Data kindly reviewed (11-JUL-1990) by Tennent J.M.
From EMBL entry ECPAP; dated 24-SEP-1990.
FEATURES Location/Qualifiers
RBS 44..47
/note="pot. ribosome binding site"
CDS 52..630
/note="precursor protein (AA -27 to 166)"
/codon_start=52
CDS 52..132
/note="signal peptide (AA -27 to -1)"
/codon_start=52
CDS 133..630
/note="mature PapJ protein (AA 1 to 166)"
/codon_start=133
BASE COUNT 150 a 146 c 195 g 142 t
ORIGIN
1 aaaaaagaga aataatgtac cgcaataacg gttaaatgcg ggtgggatat tatggttgtg
61 aataaaacaa cagcagtact gtatcttatt gcactgtcgc tgagtggttt catccatact
121 ttcctgcggg ctgaagagcg gggtatatac gatgacgtct ttactgcaga tgcgttgcgt
181 cattaccgga taaatgaacg ggggggacgc accggaagcc tgacctgcag tggtgcactg
241 ctgtcctcac cctgcacgct ggtgagtaat gaggtgccgt taagcctccg gccggaaaat
301 cactctgcgg cagccggagc acctctgatg ctgaggctgg caggatgtgg ggacggtggt
361 gcacttcagc ccggaaaacg gggcgttgcg atgacagtct ccggctcact ggtaaccggt
421 cccggaagcg gaagtgcttt acttcctgac cgtaagctat ccggctgtga ccatcttgtt
481 atacacgatg gtgatacctt tttactgtgc cggcctgacc ggcggcagga ggagatgctg
541 gccgcctgga ggaaaagggc gacacaggag ggtgagtatt ctgatgccag gagcaatccg
601 gccatgctgc gtctgagcat aaaatatgag taa
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