GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECAPEH 1618 bp ds-DNA BCT 27-MAY-1991
DEFINITION Erwinia carotovora peh gene for endo-polygalacturonase (EC
3.2.1.15)
ACCESSION X52944
KEYWORDS endo-polygalacturonase; pectolytic enzyme.
SOURCE Erwinia carotovora DNA.
ORGANISM Erwinia carotovora
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1618)
AUTHORS Hinton,J.C.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1618)
AUTHORS Hinton,J.C., Gill,D.R., Lalo,D., Plastow,G.S. and Salmond,G.P.
TITLE Sequence of the peh gene of Erwinia carotovora: homology between
Erwinia and plant enzymes
JOURNAL Mol. Microbiol. 4, 1029-1036 (1990)
STANDARD full automatic
COMMENT Data kindly reviewed (15-AUG-1990) by Hinton J.C.D.
*source: strain=SCRI193, sub-species carotovora; library=pUC8;
*source: clone=pHHX;
From EMBL entry ECPEH; dated 22-OCT-1990.
FEATURES Location/Qualifiers
misc_feature 138..175
/note="sigma-70-type promoter"
misc_feature 141..159
/note="KdgR-binding site"
RBS 281..284
/note="ribosome binding site"
CDS 291..1496
/note="precursor protein (AA -26 to 376)"
/codon_start=291
CDS 291..368
/note="signal peptide (-26 to -1)"
/codon_start=291
CDS 369..1496
/note="endo-polygalacturonase (AA 1-376)"
/codon_start=369
BASE COUNT 467 a 317 c 415 g 419 t
ORIGIN
1 aagcttacca cccgctggct tctgcgcatg acgggattcg ttttgtttcc acgtttcgtt
61 gatacttcat ttaatttttt ggtgatattt taaccatgcg taaagttgaa tgctttccga
121 ataagcattg gaataatttt atgtaaatgg acttttcatt aatctacata ggcatgtgtg
181 acaattttta gtttctataa aggtgtttta tttttcttat taagcaaaat gaaagcgtaa
241 tgccttttgg ggatggaaaa gttgtggatg cttataaggt aaggtttgac atggaatatc
301 aatcaggcaa gcgagtttta tcattatcac tggggcttat cggtttgttt agcgcatcgg
361 catttgcttc tgattcccga acggtgagtg aaccgaaagc accgtcttcc tgtacggtgc
421 ttaaagctga cagtagtacg gccacgagta caattcaaaa agcgctgaat aattgcgggc
481 aaggaaaagc ggtaaagctg agcgcaggga gttcatccgt ttttctgagc ggtccgcttt
541 ctctaccttc tggcgtgagc ttattaatcg acaaaggggt aaccctacgt gctgtgaata
601 atgccaagtc ttttgaaaat gcgccctcat cctgtggcgt ggtggataca aacggtaaag
661 gctgcgatgc gtttattacc gccacaagca cgacgaatag cggaatctat gggccgggca
721 ccattgatgg acaagggggc gtgaagcttc aggataaaaa ggtgagctgg tgggatctgg
781 ctgccgatgc caaagtgaaa aagttaaaac agaatacccc ccggctgatt cagattaata
841 agagcaagaa cttcacgctg tataacgttt ctctcattaa ttccccgaat ttccacgtcg
901 tgttcagcga tggtgacggc ttcaccgcgt ggaaaaccac gatcaaaacg ccatctaccg
961 ccagaaacac cgacggtatc gatcctatgt cgtcaaagaa catcaccatt gcccacagta
1021 atatttcgac gggtgacgat aatgtggcga tcaaagccta taaggggcgt tctgagacac
1081 gcaatatttc cattttgcat aatgaatttg gaacgggaca tggcatgtcg atcggtagcg
1141 aaacgatggg agtttataac gtgacggtcg atgatctgat tatgactggc accacgaatg
1201 gcttacgtat taaaagcgac aaatcagcag ctggcgttgt caatggtgtt cggtatagca
1261 acgtagtcat gaagaacgtg gcgaaaccga tcgtgattga cacggtatat gagaaaaaag
1321 agggaagtaa tgttcctgac tggagcgaca ttacgtttaa ggatattacg tctcaaacca
1381 aaggcgtggt ggtgctgaac ggcgagaatg cgaaaaagcc gatagaagtg acgatgaaga
1441 acgtcaaact gacgagcgac agcacatggc aaatcaagaa cgtcaccgtt aagaagtagt
1501 tcgattaata agttgttttg tcatgacaac gtccagagcg ttgacaaatc tacttgcaga
1561 acgagaacgg tggtaatggg atagaagagt aaagcgccca gggatggcgc ggctcgag
//