GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECHPELED 3562 bp ds-DNA BCT 27-MAY-1991
DEFINITION Erwinia chrysanthemi pelE and pelD genes
ACCESSION X17284
KEYWORDS lyase; pectate lyase; pelD gene; pelE gene.
SOURCE Erwinia chrysanthemi DNA.
ORGANISM Erwinia chrysanthemi
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3562)
AUTHORS Van,G.F.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3562)
AUTHORS Van,G.F.
TITLE Relationship between the pel genes of the pelADE cluster in Erwinia
chrysanthemi strain B374
JOURNAL Mol. Microbiol. 3, 1415-1424 (1989)
STANDARD full automatic
COMMENT *source: strain=B374;
From EMBL entry ECPELED; dated 11-JUL-1990.
FEATURES Location/Qualifiers
RBS 299..303
/note="ribosome binding site"
CDS 311..1522
/note="pelE precursor (AA -41 to 363)"
/codon_start=311
CDS 311..433
/note="signal peptide (AA -41 to -1)"
/codon_start=311
CDS 434..1522
/note="pelE protein (AA 1-363)"
/codon_start=434
terminator 1540..1570
/note="pelE transcription terminator"
RBS 2033..2037
/note="ribosome binding site"
CDS 2045..3217
/note="pelD precursor (AA -31 to 360)"
/codon_start=2045
CDS 2045..2137
/note="signal peptide (AA -31 to -1)"
/codon_start=2045
CDS 2138..3217
/note="pelD protein (AA 1-360)"
/codon_start=2138
terminator 3242..3266
/note="pelD transcription terminator"
BASE COUNT 1073 a 982 c 776 g 731 t
ORIGIN
1 aaaccgccag tctaaaacag cagaattaat taactatcac cgttacaatc atcgaaatta
61 aaaatataaa taattgattt aaatcataaa aaataaaaaa ccaagtaaca caaagttaca
121 aatacagtca atagtttatt ttttattaat aaacaccatt gtcatcatcg tgacaaagtt
181 cacaaaatag acactaaaac cgcataaaca ttcgccaaaa caaaagatga aatggtattt
241 cgtttttaga cacacatgta acaaatggac accatttgga tcgctcactg agcacacaag
301 gaaaattgcc atgaacaact cacgtatgtc ttccgtttca acacagaaaa caacaggacg
361 ttctgccctg ggaaccaaaa gcgcgctggc ggcgattatc gccaccacca tgatggtctc
421 agtcgcttcc gccgccagtc tgcaaaccac caaagcgaca gaagcggcgt caaccggctg
481 ggcaacgcag agcggcggca ccaccggcgg cgccaaagcg tcatcatcca aaatctacgc
541 ggtgaaaagc atcagcgaat tcaaagcagc actgaacgga accgattcgt cgcccaagat
601 catccaggtc accggagcaa ttgatatcag cggcggcaaa gcctacacca gctttgacga
661 tcagaaggcc cgcagccaaa tcagcattcc gtccaacacc accatcatcg gtattggcaa
721 caaaggcaaa ttcaccaacg gttcgctggt ggtgaaaggc gtcagcaacg ttatcctgcg
781 taacctgtac atcgaaacgc cggtggacgt ggcgccgcat tacgaagagg gagatggctg
841 gaacgccgag tgggacgcag tagtgattga cagcacagac cacgtctggg ttgaccatgt
901 caccatcagc gacggcagct tcaccgacga caaatacacc accaaaaacg gcgaaaaata
961 tgtgcagcat gacggctcgc tggatatcaa gcgcggctcc gactacgtta ccgtttctaa
1021 tagccgcttc gagctgcacg acaaaaccat cctgatcggc cacagcgaca acaacggctc
1081 gcaggacgcc ggcaaactgc gcgtcacctt ccacaacaac ctgtttgacc gggtcggcga
1141 acgtaccccg cgcgtacgct tcggcagcgt tcacgcttac aacaacgttt acgttggcga
1201 cgtcaaccac aaagcctatc gctatcagta cagcttcggt atcggcacca gcggtagcct
1261 gctgtctgag tccaacgcgt ttaccattga taacatgaaa aaaatcagcg gccgcgacaa
1321 agaatgcagc gtggtcaagg cgtttaacgg taagatcttc tctgataaag gctcgatcat
1381 caacggcgct tcgtacaatc tgaatggttg cggctttggc ttcagtgcat acagcgccaa
1441 gatcccgtac aaatactcag cccaaaccat caccaccagc ctggctggca gcatcagcag
1501 caacgcgggc tacggtaaac tgtaatttcc cgcccagcgt aaaggccact tgcaaaagtg
1561 gcctttattt tcccatattc ccaccactcg tattaccccg atgttctccg aagcgccact
1621 caatacggtt caggttaatc cggccttgcc acaggcgttc atcaagaaat cgaccggttt
1681 tccccatcaa atgcagggac tagcaggaat tagtaagagc cacagattaa taaaattcta
1741 tttaataaaa atcgaatcaa ttgttaactg tttgggttca tttcagataa aaacacttat
1801 acatatagct gaatttaaaa gaaaaattaa ttcaacattc ataaccaaaa gttaccggtc
1861 acgatcacac tttagataaa attaatcagc ctcataaaaa aaacgagatt ttgatcacaa
1921 aataaacaat cgaaaacgct taaaaatccg ccttgccaaa ggacaaaatg gcgtttcatt
1981 tttttcacaa acacttttca gtcaacaaaa ttggattagc gcagatagcg caaggaacca
2041 gtctatgaac aacacacgag tgtctttccg tagtaccaaa agcttgctgg cagccatcat
2101 cgctacatcg atgatgacct ggtcagtcaa tcgagccacg ctgcaaacca ccaaggcgac
2161 agaagcagcg tcaactggct gggcaacgca gggcggcacc accggcggcg ctaaagcagc
2221 ctcagccaaa atctatgcag taaaaaatat cagcgaattt aaagccgcgc tgaatggcac
2281 cgacaccgat ccgaaaatca ttcaggtgac cggcgctatc gatatcagcg gcggcaaagc
2341 ctacaccagc tttgacgatc agaaagcccg cagccagatc agcgttccgt ccaacaccac
2401 catcatcggt attggcagca atggcaaatt caccaacggc tctctggtca tcaaaggcgt
2461 gagtaacgtt atcctgcgta acctgtatat cgaaacgccg gtggatgtag caccgcatta
2521 cgaggaaggg gatggctgga acgccgagtg ggatgcggcg gtgatcgaca attccacccg
2581 cgtttgggtt gaccatgtca ccatcagcga cggcagcttc accgatgaca aatacaccac
2641 caaaaacggc gaaaaatatg tgcagcacga cggcgcgctg gatatcaaga aagggtccga
2701 ctacgtcacc atttccagca gccgcttcga actgcatgac aaaaccatcc tgatcggcca
2761 tagcgacagc aacggctcac aggactccgg caaactgcgc gttaccttcc acaacaacgt
2821 gtttgaccgt gtaaccgaac gtaccccgcg cgtacgcttt ggtagcatcc acgcgtataa
2881 caacgtgtat ctgggcgacg tgaaaaacag cgtctatccg tatctgtaca gcttcggtct
2941 tggcaccagc ggcacgatcc tgtcggagtc caactccttc acgctctcca acctgaagag
3001 cattgatggc aaaaatccag aatgcagcat cgtgaagcaa ttcaacagca aggtattctc
3061 cgataacggc tcactggtta acggctcgtc aaccactaag ctggacactt gcgccgtgac
3121 ggcttacaaa ccgactctgc cgtacaaata ttcggctcag accatgacaa gtagcctggc
3181 tagcagcatc aatagcaatg caggatacgg caaactgtaa ttcctctgcc agctaaatcg
3241 cggccacttg aaaaagtggc cttttttgca tcaatcgcag gtaaaagagg tcatcatcat
3301 gctcacaact acctggaatc gcgccttttt tctcggttcc ctgctttgtc tgccgatatc
3361 ttttgcgcag gctgaaggca ccgtgacgga aaccaacgct tcacctacgc cccacgagct
3421 caatgtcgtc acgctggctc ccaatacatc gatcagcggc agagtggcct accgtgacat
3481 acgcttccct gccacattac tcatcaaaga tcagcgtggc gtagaacgca acgtaaaaac
3541 cgatattcaa gggcgatttt at
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