GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOPOLD 4081 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli polB gene for DNA polymerase II, and open reading frame
(partial).
ACCESSION X54847
KEYWORDS DNA polymerase II; polB gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4081)
AUTHORS Ishino,Y.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT *source: strain=K12; isolate=W3110; **map: map position=1.4min
From EMBL entry ECPOLB; dated 06-JAN-1991.
FEATURES Location/Qualifiers
-35_signal 241..246
-10_signal 265..270
RBS 289..291
mat_peptide 300..2648
/product="DNA polymerase II"
/gene="polB"
CDS 300..2651
/product="DNA polymerase II"
/gene="polB"
/codon_start=300
mat_peptide 2811..>4018
CDS 2811..>4018
/codon_start=2811
BASE COUNT 942 a 1103 c 1133 g 903 t
ORIGIN
1 atcgatgcag cgcaaatgcc cggcgttctg gtccattccc acggcccgtt tgcatggggc
61 aaaaatgccg aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg
121 atattctgcc gtcagttagc gccgcagtta ccggatatgc agcaaacgct gctggataaa
181 cactatctgc gtaagcatgg cgcgaaggca tattacgggc agtaatgact gtataaaacc
241 acagccaatc aaacgaaacc aggctatact caagcctggt tttttgatgg attttcagcg
301 tggcgcaggc aggttttatc ttaacccgac actggcggga caccccgcaa gggacagaag
361 tctccttctg gctggcgacg gacaacgggc cgttgcaggt tacgcttgca ccgcaagagt
421 ccgtggcgtt tattcccgcc gatcaggttc cccgcgctca gcatattttg cagggtgaac
481 aaggctttcg cctgacaccg ctggcgttaa aggattttca ccgccagccg gtgtatggcc
541 tttactgtcg cgcccatcgc caattgatga attacgaaaa gcgcctgcgt gaaggtggcg
601 ttaccgtcta cgaggccgat gtgcgtccgc cagaacgcta tctgatggag cggtttatca
661 cctcaccggt gtgggtcgag ggtgatatgc acaatggcac tatcgttaat gcccgtctga
721 aaccgcatcc cgactatcgt ccgccgctca agtgggtttc tatagatatt gaaaccaccc
781 gccacggtga gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc gtttatatgc
841 tggggccgga gaatggcgac gcctcctcgc ttgatttcga actggaatac gtcgccagcc
901 gcccgcagtt gctggaaaaa ctcaacgcct ggtttgccaa ctacgatcct gatgtgatca
961 tcggttggaa cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc gagcgttacc
1021 gtcttccgct gcgtcttggg cgcgataata gcgagctgga gtggcgcgag cacggcttta
1081 aaaacggcgt cttttttgcc caggctaaag gtcggctaat tatcgacggt atcgaggcgc
1141 tgaaatccgc gttctggaat ttctcttcat tctcgctgga aactgtcgct caggagctat
1201 taggcgaagg aaaatctatc gataacccgt gggatcgaat ggacgaaatt gaccgccgtt
1261 tcgccgaaga taaacctgcg ctggcaactt ataacctgaa agattgcgag ctggtgacgc
1321 agatcttcca caaaactgaa atcatgccat ttttactcga acgggcaacg gtgaacggcc
1381 tgccggtgga ccgacacggc ggttcggtgg cggcatttgg tcatctctat tttccgcgaa
1441 tgcatcgcgc tggttatgtc gcgcctaatc tcggcgaagt gccgccgcac gccagccctg
1501 gcggctacgt gatggattca cggccagggc tttatgattc agtgctggtg ctggactata
1561 aaagcctgta cccgtcgatc atccgcacct ttctgattga tcccgtcggg ctggtggaag
1621 gcatggcgca gcctgatcca gagcacagta ccgaaggttt tctcgatgcc tggttctcgc
1681 gagaaaaaca ttgcctgccg gagattgtga ctaacatctg gcacgggcgc gatgaagcca
1741 aacgccaggg taacaaaccg ctgtcgcagg cgctgaaaat catcatgaat gccttttatg
1801 gcgtgctcgg caccaccgcc tgccgcttct tcgatccgcg gctggcatcg tcgatcacca
1861 tgcgtggtca tcagatcatg cggcaaacca aagcgttgat tgaagcacag ggctacgacg
1921 ttatctacgg cgataccgac tcaacgtttg tctggctgaa aggcgcacat tcggaagaag
1981 aagcggcgaa aatcggtcgt gcactggtgc agcacgttaa cgcctggtgg gcggaaacgc
2041 tgcaaaaaca acggctgacc agcgcattag aactggagta tgaaacccat ttctgccgtt
2101 ttctgatgcc aaccattcgc ggagccgata ccggcagtaa aaagcgttat gccggactga
2161 ttcaggaggg cgacaagcag cggatggtgt ttaaagggct ggaaaccgtg cgcaccgact
2221 ggacgccgct ggcccagcag tttcagcagg agctatacct gcgcatcttc cgcaacgagc
2281 catatcagga atatgtacgc gaaaccatcg acaaactgat ggcgggtgaa ctggatgcgc
2341 gactggttta ccgtaaacgc cttcgccgtc cgctgagcga gtatcagcgt aatgtgccgc
2401 ctcatgtacg cgccgctcgc cttgccgatg aagaaaacca aaagcgtggt cgccccttgc
2461 aatatcagaa tcgcggcacc attaagtacg tatggaccac caacggcccg gagccgctgg
2521 actaccaacg ttcaccactg gattacgaac actatctgac ccgccagcta caacccgtgg
2581 cggagggaat actccctttt attgaggata attttgctac acttatgacc gggcaacttg
2641 ggctattttg agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg acgaatgctt
2701 tgccatccag taccatagcg ccctttccat tcctggacct gaataacacc actacctcat
2761 aagcacggta gcgggtggtt attgcctgca attaaagata tagagccgaa cacatatgcc
2821 ttttacactt ggtcaacgct ggatcagcga tacagaaagc gaattgggac ttggaaccgt
2881 tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca tctactggtg aaaaccgtct
2941 gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc aaccctggtg ataccattac
3001 cagccatgac ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac
3061 ctatatcggt actcgcctgg atactgaaga gtccggcgta gccctgcgtg aagttttcct
3121 tgatagcaaa ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc agattgaccg
3181 tatggaccgc tttgcgctgc gttatcgcgc gcgtaaatat tccagcgaac agttccgtat
3241 gccgtacagc ggcctgcgcg gtcagcgtac cagcctgatc ccgcatcagc tcaacatcgc
3301 tcatgatgtt ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg
3361 gaaaaccatt gaagccggga tgatcctgca tcagcaactg ctctctggcg ctgctgaacg
3421 tgtgctaatt atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg
3481 tttcaacctg cgctttgcgc tatttgatga tgagcgttat gccgaagctc agcacgatgc
3541 ttacaacccg tttgacaccg aacagctggt gatttgctcg ctggattttg cccgtcgtag
3601 caaacagcgc ctggaacatc tctgtgaagc cgaatgggac ctgctggtgg tcgatgaagc
3661 gcatcacctg gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca ttgaacaact
3721 ggcagagcac gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc tggggatgga
3781 aagccacttc gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt
3841 cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc tgctggcagg
3901 taacaaactg agcaatgacg aactgaacat gctcggcgag atgatcggcg agcaggatat
3961 cgagccgctg ttgcaggcag caaacagcga cagcgaagat gcccagagcg cccgtcagga
4021 gctggtttcg atgctgatgg atcgccacgg caccagccgc gtgctgttcc gtaacacgcg
4081 t
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