GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOPRRBCD 3853 bp ds-DNA BCT 27-MAY-1991
DEFINITION Escherichia coli prrB, prrC and prrD genes
ACCESSION X52284
KEYWORDS nuclease; prrB gene; prrC gene; prrD gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3853)
AUTHORS Kaufmann,G.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3853)
AUTHORS Levitz,R., Chapman,D., Amitsur,M., Green,R., Snyder,L. and
Kaufmann,G.
TITLE The optional E.coli prr locus encodes a latent form of phage T4-
induced anticodon nucelase
JOURNAL EMBO J. 9, 1383-1389 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17222; PRRB$ECOLI. SWISS-PROT; P17223; PRRC$ECOLI.
SWISS-PROT; P17224; PRRD$ECOLI.
*source: strain=CTr5X; **map: map position=29 min;
From EMBL entry ECPRRBCD; dated 28-MAY-1990.
FEATURES Location/Qualifiers
promoter 50..55
/note="pot. promoter region"
promoter 248..253
/note="pot. promoter region"
RBS 424..427
/note="pot. ribosome binding site"
CDS 435..1637
/note="prrB protein (AA 1-401)"
/codon_start=435
promoter 1577..1582
/note="pot. promoter region"
RBS 1642..1646
/note="pot. ribosome binding site"
CDS 1653..2840
/note="prrC protein (AA 1-396)"
/codon_start=1653
promoter 2569..2574
/note="pot. promoter region"
RBS 2824..2828
/note="pot. ribosome binding site"
CDS 2836..3774
/note="prrD protein (AA 1-313)"
/codon_start=2836
BASE COUNT 1183 a 814 c 858 g 998 t
ORIGIN
1 gtcgacaata actatgtcga aaccgtgatt tcactggcac cgaatctgtt ctttggcacc
61 accattgccg tcaatattct ggtgctgtct aaacataaaa ccgataccaa agttcagttt
121 attgacgcca gcgaactatt caaaaaagag accaacaaca atatcctgac cgatgcccat
181 atcgaacaga ttatgcaggt atttgccagc aaggaagatg ttgctcatct ggcgaaatca
241 gtcgcgtttg agaccgttgt tgctaatgac tataacctgt cggtgagcag ctatgttgaa
301 gcgaaagata ctcgcgaaat tatcgatatc gctgagttga atgcagagct gaaaaccacg
361 gtcagcaaaa tcgaccagtt gcgtaaagat attgatgcaa ttgtggctga aattgaaggc
421 tgcgaggtgc agaaatgagc gagttgagtt atctggaaaa attgatggat ggggttgaag
481 ttgagtggtt gccactctca aaagtattca acttaaggaa tggttataca ccgtctaaaa
541 ctaaaaaaga attttgggcg aatggtgata taccttggtt cagaatggat gacatacgtg
601 aaaatggtag aattctaggg agttcacttc aaaaaatttc atcctgcgcc gtaaaagggg
661 ggaaactatt tcctgaaaat tcaatattaa tatcaacgtc ggcaacaatt ggtgagcatg
721 ctttaatcac tgtacctcat ctagccaatc aaaggtttac atgtttggct ttaaaagagt
781 cttatgctga ttgctttgat attaaatttt tattttacta ctgtttttct ttagctgagt
841 ggtgccgaaa aaacacaacg atgtccagtt ttgcgtctgt tgatatggat ggattcaaaa
901 aatttttaat ccctcgcccc tgcccggata atccggaaaa atcccttgcc atccagtctg
961 aaatcgtgcg aattctggac aaattcagcg cccttactgc agagcttact gcagagctta
1021 ctgcagagct tagcatgcgt aaaaaacaat acaactacta tcgcgaccag ttgttgagtt
1081 ttaaagagga tgaggttgag ggtaagagga aaaccttagg ggaaattatg aagatgcgtg
1141 ccggacaaca tatttcggct cataatataa ttgaaagaaa agaagagagt tatatatatc
1201 cttgttttgg aggaaatggc atacggggtt acgtcaaaga aaaaagtcat gatggagaac
1261 atctattaat agggcgacaa ggagcattgt gtggtaatgt tcaaagaatg aaaggccagt
1321 tctatgctac agaacatgct gttgtagttt cagtcatgcc cgggattaac attgactggg
1381 catttcatat gttaacggca atgaatctaa accaatatgc ttcaaaatcg gcacaaccgg
1441 gacttgctgt agggaaatta caggaattga aattatttgt cccttcaata gagagacaaa
1501 tatatattgc cgcaatactc gataaattcg acactctgac caactccatc accgaagtct
1561 cccgcgtgaa atcgagttgc gccagaaaca gtacgaatat tatcgagata tgctatttag
1621 tttcccgaag ccggaagtag cagaggcatg acatgggcaa gacactctcg gaaatagctc
1681 aacagcttag tacgcctcag aaagtcaaga agactgtcca taaagaagtt gaagcgacca
1741 gggccgtgcc aaaggtgcag ttaatctacg ctttcaatgg cacggggaaa acgcgtcttt
1801 cccgggattt caagcaactg cttgagtcca aagttcatga cggagaaggt gaagacgaag
1861 ctgaacagtc cgcgttgtcg cgcaaaaaaa tcctctatta taatgccttc accgaggact
1921 tgttctattg ggataacgat ctgcaagaag acgcagaacc gaagctgaag gtgcagccta
1981 actcctatac aaactggttg ctgaccttgc taaaagattt gggacaggat agcaatatcg
2041 tccgttattt ccagcgctat gcgaacgaca agctaacgcc acacttcaat ccagatttta
2101 ccgaaatcac tttttccatg gagcgcggta acgatgagcg ttccgcccac atcaagttat
2161 ccaaaggtga agagagcaac ttcatctgga gtgtgtttta caccctactg gatcaagtgg
2221 taacaattct caatgtcgct gacccggatg cgcgcgagac ccacgcattt gatcaactga
2281 aatatgtatt cattgatgat cccgttagct cccttgatga taaccatttg attgaactgg
2341 cggttaatct tgcagggctg atcaaatcca gcgaatccga tttgaagttc attatcacga
2401 cacatagtcc aatattttat aatgtacttt tcaatgagtt gaacggtaaa gtttgctaca
2461 tgttggaaag ttttgaggat ggtacatttg ccctcacaga aaaatatggc gactctaaca
2521 agagtttttc ctaccatctc cacctaaagc aaacaatcga acaggcaatt gctgacaaca
2581 acgttgaaag atatcatttc acgttactgc gcaatcttta tgagaaaacg gccagctttc
2641 tgggctaccc taaatggtct gaactcttgc ctgacgacaa gcagctttat ctgagcagaa
2701 tcatcaattt cacaagccac agcacgctat cgaatgaggc cgttgcagag ccaacgccag
2761 cggagaaagc gactgtcaaa ctgttgctcg atcacttgaa gaacaactgt ggcttctggc
2821 agcaggaaca aaaaaatggt tgattgcacc aaaccaattg ctgagtccaa taattttatc
2881 attttggaca aatacaaccc ggactggaag attaccgaga gctaccagag cgaaggtgat
2941 ctggagcgtg agctgattca ggatttggtt aaccagggct atgaatacct gccaaccctg
3001 aacaacacca aggctatgct cgctaatgtc agggaacagt tgcaaaacct taacaatgta
3061 gagttcctgg aggctgaatg gcgtcgcttt gtggaaacct ggatggacaa gcccagcgat
3121 ggcgttgttg aaaaggcccg caagattcat gatgattatg ttcatgattt cgtcttcgac
3181 gatggccgta ttcagaatat ctatctgctg gatagaaaaa acatccttcg caataaagtg
3241 caggtcatca agcagtttga acaagcaggc acgcacgcca atcgctacga tgtcaccatt
3301 ctggtcaatg gcctgccgct ggtacaaatt gaattgaaaa aacgtggtgt agcgattcgt
3361 gaggctttca accagataca tcgttacagt aaagagagtt ttaacagcga aaattccctg
3421 tttaagtatc tgcagctgtt cgtcatttcc aacggcactg atacccgtta ttttgccaac
3481 accacaaagc gcgataaaaa cagttttgac ttcaccatga actgggctaa atcagacaat
3541 acactgatta aagacctcaa agactttacc gctacctttt tccagaaaca tactctgctt
3601 aatgttctgg tgaactacag cgtttttgac agcagtcaga cgctactggt aatgcgaccg
3661 taccagattg ccgccaccga gcgcattctg tggaaaatta agagttcctt taccagcgaa
3721 gaactggtca aaaccggaaa gcggtgggta tatctggcac actaccggtt ctggtaaaac
3781 cctcaccagc tttaaagccg cgcgtctggc aacagaactg gactttattg ataaagtttt
3841 ctttgtggtc gac
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