GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECORNE 3178 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli rne gene for RNase E
ACCESSION X54309
KEYWORDS ribonuclease E; rne gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3178)
AUTHORS Chauhan,A.K.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Chauhan,A.K., Miczak,A., Taraseviciene,L. and Apirion,D.
TITLE Sequencing and expression of the rne gene of Escherichia coli
JOURNAL Nucleic Acids Res. 19, 125-129 (1991)
STANDARD full staff_review
COMMENT *source: strain=K12 **map: map position=24.2 mins
From EMBL entry ECRNE; dated 10-JAN-1991.
FEATURES Location/Qualifiers
promoter 620..625
/note="-35 region"
promoter 648..653
/note="-10 region"
RBS 657..660
/note="ribosome binding site"
CDS 666..1688
/note="RNase E (AA 1 - 341)"
/codon_start=666
old_sequence 1598..1600
/note="cgg was cg [1]"
old_sequence 2531..2534
/note="cgcg was cgg [1]"
BASE COUNT 813 a 854 c 834 g 677 t
ORIGIN
1 aattctcatg tttgacagct tatcatcgat acgttgcccc gcttcgtcag cggtgatagc
61 aacaattttt acggatggag tctctgtttt catggttggc gattctaatt agccaacagg
121 attcgcgcca ctcatttttc tatgcttata tttacttttg caccttatta cttcactgcg
181 tgatcacttt attgatggtt attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt
241 aagcgggtga taaatggtaa aagtcatctt gctataacaa ggcttgcagt ggaataatga
301 ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt acggaataac ccattttgcc
361 cgaccgatca tccacgcagc aatggcgtaa gacgtattga tctttcaggc agttagcggg
421 ctgcgggttg cagtccttac cggtagatgg aaatatttct ggagagtaat acccagtctg
481 tttctttgat aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct
541 ctttgagctg acgataaccg tgaggttggc gacgcgacta gacacgaggc catcggttca
601 cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag
661 ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga agagttgcgc gttgcccttg
721 tagatgggca gcgtctgtat gacctggata tcgaaagtcc agggcacgag cagaaaaagg
781 caaacatcta caaaggtaaa atcacccgca ttgaaccgag tctggaagct gcttttgttg
841 attacggcgc tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc gaatatttcc
901 ctgctaacta cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg
961 aagtcattgt tcagatcgat aaagaagagc gcggcaacaa aggcgcggca ttaaccacct
1021 ttatcagtct ggcgggtagc tatctggttc tgatgccgaa caacccgcgc gcgggtggca
1081 tttctcgccg tatcgaaggc gacgaccgta ccgaattaaa agaagcactg gcaagccttg
1141 aactgccgga aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg
1201 cgctgcaatg ggatttaagc ttccgtctga aacactggga agccatcaaa aaagccgctg
1261 aaagccgccc ggccccgttc ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc
1321 gcgattactt acgtcaggac atcggcgaaa tccttatcga taacccgaaa gtgctcgaac
1381 tggcacgtca gcatatcgct gcattaggtc gcccggattt cagcagcaaa atcaaactga
1441 acaccggcga gatcccgctg ttcagccact accagatcga gtcacagatc gagtccgcct
1501 tccagcgtga agttcgtctg ccgtctggtg gttccattgt tatcgacagc accgaagcgt
1561 taacggccat cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc gaagaaaccg
1621 cgtttaacac taacctcgaa gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc
1681 tcggcggcct gattgttatc gacttcatcg acatgacgcc agtacgccac cagcgtgcgg
1741 tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata
1801 tttctcgctt tggcctgctg gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat
1861 ccagtcatca cgtttgtccg cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc
1921 tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct gaaagagaac acccaggaag
1981 ttcacgccat tgttcctgtg ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg
2041 taaatgccat tgaaactcgt caggacggtg tgcgctgtgt aattgtgcca aacgatcaga
2101 tggaaacccc gcactaccac gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa
2161 gctacatgct gccgaagctg catgaagaag cgatggcgct gccgtctgaa gaagagttcg
2221 ctgaacgtaa gcgtccggaa caacctgcgc tgcaaccttt gccatgccgg atgtgccgcc
2281 tgcgccaacg ccagctgaac ctgccgcgcc tgttgtagct ccagcaccga aagctgcacc
2341 ggcaacacca gcagctcctg cacaacctgg gctgttgagc cgcttcttcg gcgcactgaa
2401 agcgctgttc agcggtggtg aagaaaccaa accgaccgag caaccagcac cgaaagcaga
2461 agcgaaaccg gaacgtcaac aggatcgtcg caagcctcgt cagaacaacc gccgtgaccg
2521 taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa
2581 ccgtcgtaat cgtcgccagg cacagcagca gactgccgag acgcgtgaga gccgtcagca
2641 ggctgaggta acggaaaaag cgtaccgccg acgagcagca agcgccgcgt cgtgaacgta
2701 gccgccgccg taatgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg
2761 aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc
2821 gtaaacagcg tcagctcaat cagaagtgcg ttacgagcaa agcgtagccg aagaagcggt
2881 agtcgcaccg gtggttgaag aaactgtcgc tgccgaacca attgttcagg aagcgccagc
2941 tccacgcaca gaactggtga aagtcccgct gccagtcgta gcgcaaactg caccagaaca
3001 gcaagaagag aacaatgctg ataaccgtga caacggtggc atgcgctcgt tctcgccgct
3061 cgcctcgtca cctgcgcgta agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
3121 caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactc gcgtctgg
//