GenBank-Updates@genbank.bio.net (02/08/91)
LOCUS ECOTRMA 1684 bp ds-DNA BCT 08-FEB-1991
DEFINITION E.coli tRNA(m-5-U54)methyltransferase (trmA) gene, complete cds and
vitamin B-12 binding protein (btuB) gene, 5' end.
ACCESSION M57568
KEYWORDS methyl transferase; tRNA modifying enzyme;
tRNA(m-5-U54)methyltransferase.
SOURCE E.coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1684)
AUTHORS Gustafsson,C., Lindstroem,P.H., Hagervall,T.G., Esberg,B. and
Bjoerk,G.R.
TITLE The trmA promoter has regulatory features and sequence elements in
common with the ribosomal RNA P1 promoter family in Esherichia
coli.
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
mRNA complement(1..256)
/gene="btuB"
/evidence=EXPERIMENTAL
/partial
CDS complement(1..16)
/gene="btuB"
/evidence=EXPERIMENTAL
/codon_start=1
/partial
/product="vitamin B-12 binding protein"
mRNA 360..1517
/gene="trmA"
/evidence=EXPERIMENTAL
CDS 385..1485
/EC_number="2.1.1.35"
/gene="trmA"
/evidence=EXPERIMENTAL
/codon_start=385
/product="tRNA(m-5-U54)methyltransferase"
BASE COUNT 455 a 421 c 422 g 386 t
ORIGIN
1 aagctttttt aatcattgta aagcatccac aatagaagaa ggatgccgca ggtttcatca
61 atattacgcg atgatgagaa ccagatgcga cgttggccgg caggtcttcg ggcttggagg
121 ggtatctaag atactaagag atgatgactt cccaccgaat ggcagtgtcc gcataacgca
181 atcatcgcac ctttccttac cgctgcgcgt cagctccaga ttcgcactgg attccctatt
241 aactcacagg accggcaagt ggatgctaca ggttgtaaca agttactgtc cagacgtagc
301 tcacaaatag gaattcatca agatctggac atctgatgag caatccctac aatcgccgcg
361 tactttaatt tttcaggata catcatgacc cccgaacacc ttccaacaga acagtatgaa
421 gcgcagttag ccgaaaaagt ggtacgtttg caaagtatga tggcaccgtt ttctgacctg
481 gttccggaag tgtttcgctc gccggtcagt cattaccgga tgcgcgcgga gttccgcatc
541 tggcacgatg gcgatgacct gtatcacatc attttcgatc aacaaaccaa aagccgcatc
601 cgcgtggata gcttccccgc cgccagtgaa cttatcaacc agttgatgac ggcgatgatt
661 gcgggtgtgc gtaataatcc cgttctgcgc cacaagttgt tccagattga ttacctcact
721 acactgagta atcaggcggt ggtttccctg ctataccata agaagctgga tgatgagtgg
781 cgtcaggaag cggaggccct gcgcgatgca ctgcgcgcgc agaatctgaa tgtgcatctg
841 attggtcggg caacgaaaac caaaatcgag ctggatcagg attacatcga tgaacgtctg
901 ccggtcgcag ggaaagagat gatctaccgt caggtagaaa acagctttac ccagccgaac
961 gcggcgatga atattcagat gctggaatgg gcgctggacg taaccaaagg ctcaaaaggc
1021 gatttactgg agctgtactg cggcaacggt aacttttcat tagcgctggc gcgtaatttt
1081 gatcgggtat tagccaccga aatcgctaag ccgtcggttg ctgctgcgca atacaacatc
1141 gcagctaacc atattgataa cgtacaaatt attcgtatgg cggcagaaga atttactcag
1201 gcgatgaatg gtgtgcgcga gtttaaccgc ctgcaaggga tcgacttaaa gagttatcag
1261 tgcgaaacca tttttgtcga ccctccgcgc agcggtctgg acagtgaaac cgagaaaatg
1321 gtgcaggcgt atccgcgtat tttgtacatc tcctgtaacc cggaaacgtt atgcaagaat
1381 ctggaaacat taagccagac gcacaaggtc gaacgtctgg ctctgtttga tcagttcccc
1441 tacacgcacc atatggagtg cggcgtatta ctgaccgcga agtaaaaccc atgcggatgc
1501 gccagcatcc ggcataatac cgattactca gcaacttcct gcttacggtt acgccatctt
1561 cgcgccaatc cagaacacca taatgacgag agtactgccg gcaagaagtt agagccgata
1621 tccggatatt ccgcacgaac taccgtgctg tacagcagta cgccgagaat aaaacaggca
1681 gctg
//GenBank-Updates@genbank.bio.net (03/19/91)
LOCUS ECOTRMA 1684 bp ds-DNA BCT 19-MAR-1991
DEFINITION E.coli tRNA(m-5-U54)methyltransferase (trmA) gene, complete cds and
vitamin B-12 binding protein (btuB) gene, 5' end.
ACCESSION M57568
KEYWORDS methyl transferase; tRNA modifying enzyme;
tRNA(m-5-U54)methyltransferase.
SOURCE E.coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1684)
AUTHORS Gustafsson,C., Lindstroem,P.H., Hagervall,T.G., Esberg,K.B. and
Bjoerk,G.R.
TITLE The trmA promoter has regulatory features and sequence elements in
common with the rRNA P1 promoter family of Escherichia coli
JOURNAL J. Bacteriol. 173, 1757-1764 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
mRNA complement(1..256)
/gene="btuB"
/evidence=EXPERIMENTAL
/partial
CDS complement(1..16)
/product="vitamin B-12 binding protein"
/gene="btuB"
/evidence=EXPERIMENTAL
/codon_start=16
/partial
mRNA 360..1517
/gene="trmA"
/evidence=EXPERIMENTAL
CDS 385..1485
/EC_number="2.1.1.35"
/product="tRNA(m-5-U54)methyltransferase"
/gene="trmA"
/evidence=EXPERIMENTAL
/codon_start=385
BASE COUNT 455 a 421 c 422 g 386 t
ORIGIN
1 aagctttttt aatcattgta aagcatccac aatagaagaa ggatgccgca ggtttcatca
61 atattacgcg atgatgagaa ccagatgcga cgttggccgg caggtcttcg ggcttggagg
121 ggtatctaag atactaagag atgatgactt cccaccgaat ggcagtgtcc gcataacgca
181 atcatcgcac ctttccttac cgctgcgcgt cagctccaga ttcgcactgg attccctatt
241 aactcacagg accggcaagt ggatgctaca ggttgtaaca agttactgtc cagacgtagc
301 tcacaaatag gaattcatca agatctggac atctgatgag caatccctac aatcgccgcg
361 tactttaatt tttcaggata catcatgacc cccgaacacc ttccaacaga acagtatgaa
421 gcgcagttag ccgaaaaagt ggtacgtttg caaagtatga tggcaccgtt ttctgacctg
481 gttccggaag tgtttcgctc gccggtcagt cattaccgga tgcgcgcgga gttccgcatc
541 tggcacgatg gcgatgacct gtatcacatc attttcgatc aacaaaccaa aagccgcatc
601 cgcgtggata gcttccccgc cgccagtgaa cttatcaacc agttgatgac ggcgatgatt
661 gcgggtgtgc gtaataatcc cgttctgcgc cacaagttgt tccagattga ttacctcact
721 acactgagta atcaggcggt ggtttccctg ctataccata agaagctgga tgatgagtgg
781 cgtcaggaag cggaggccct gcgcgatgca ctgcgcgcgc agaatctgaa tgtgcatctg
841 attggtcggg caacgaaaac caaaatcgag ctggatcagg attacatcga tgaacgtctg
901 ccggtcgcag ggaaagagat gatctaccgt caggtagaaa acagctttac ccagccgaac
961 gcggcgatga atattcagat gctggaatgg gcgctggacg taaccaaagg ctcaaaaggc
1021 gatttactgg agctgtactg cggcaacggt aacttttcat tagcgctggc gcgtaatttt
1081 gatcgggtat tagccaccga aatcgctaag ccgtcggttg ctgctgcgca atacaacatc
1141 gcagctaacc atattgataa cgtacaaatt attcgtatgg cggcagaaga atttactcag
1201 gcgatgaatg gtgtgcgcga gtttaaccgc ctgcaaggga tcgacttaaa gagttatcag
1261 tgcgaaacca tttttgtcga ccctccgcgc agcggtctgg acagtgaaac cgagaaaatg
1321 gtgcaggcgt atccgcgtat tttgtacatc tcctgtaacc cggaaacgtt atgcaagaat
1381 ctggaaacat taagccagac gcacaaggtc gaacgtctgg ctctgtttga tcagttcccc
1441 tacacgcacc atatggagtg cggcgtatta ctgaccgcga agtaaaaccc atgcggatgc
1501 gccagcatcc ggcataatac cgattactca gcaacttcct gcttacggtt acgccatctt
1561 cgcgccaatc cagaacacca taatgacgag agtactgccg gcaagaagtt agagccgata
1621 tccggatatt ccgcacgaac taccgtgctg tacagcagta cgccgagaat aaaacaggca
1681 gctg
//GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTRNYSU 1655 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli tRNA(1)-Tyr amber-suppressor su3+ gene with two
supressor-negative derivatives (9311 upstream and 9313 downstream)
ACCESSION X04168
KEYWORDS amber suppressor; suppressor transfer RNA; transfer RNA;
transfer RNA-Tyr.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1655)
AUTHORS McCorkle,G.M. and Altman,S.
TITLE Large deletion mutants of Escherichia coli tRNA-Tyr
JOURNAL J. Mol. Biol. 155, 83-103 (1982)
STANDARD full automatic
COMMENT From EMBL entry ECTRNYSU; dated 09-MAR-1987.
FEATURES Location/Qualifiers
misc_feature 1..516
/note="9311 DNA"
tRNA 517..643
/note="mat. amber suppressor tRNA(1)-Tyr su3+"
repeat_region 625..802
/note="178 bp repeat unit"
misc_feature 625..723
/note="region deleted in 9311 and 9313"
repeat_region 803..980
/note="178 bp repeat unit"
misc_feature 850..1027
/note="region deleted in 9313"
repeat_region 981..1158
/note="178 bp repeat unit"
misc_feature 1184..1655
/note="9313 DNA"
BASE COUNT 449 a 473 c 401 g 332 t
ORIGIN
1 gatcaaaaga tggcggatgc cattgatgct tatcaacctg actacgtggt gctggcgaag
61 tatatgcggg tattaacgcc ggaatttgtg gcacgcttcc cgaataagat catcaatatt
121 caccattcat tcctgccagc gtttattggc gcacgtcctt atcaccaggc ctatgaacgt
181 ggtgtgaaga ttattggcgc aaccgctcac tatgtgaatg acaatctgga cgaaggccca
241 atcatcatgc aggacgttat tcatgtcgat catacctaca cagctgaaga tatgatgcgc
301 gcaggtcgtg acgtcgagaa aaacgtctta agtcgtgcac tatacaaagt actggcacag
361 cgcgtctttg tttacggtaa tcgaacgatt attctttaat cgccagcaaa aataactggt
421 tacctttaat ccgttacgga tgaaaattac gcaaccagtt catttttctc aacgtaacac
481 tttacagcgg cgcgtcattt gatatgatgc gccccggctt cccgataagg gagcaggcca
541 gtaaaagcat taccccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
601 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccc
661 tgaacttccc aacgaatccg caattaaata ttctgcccat gcggggaagg atgagaagct
721 tcgaccaagg ttcgactcga gcgccagcga gagagcgttg ccgcaggcaa cgacccgaag
781 ggcgaagcgc gcagcgctga gtaatccttc ccccaccacc atcactttca aaagtccctg
841 aactctcaag cgaatccgca atcaaatatt ctgcccatgc ggggaaggat gagaagcttc
901 gactaaggtt cggctcgagc gtcagcgaga gagcgttgcc gcaggcaacg acccgaaggg
961 cgaagcgcgc agcgctgagt aatccttccc ccaccaccat cactttcaaa agccccggaa
1021 ttctcaaacg aatccgcaat caaatattct gcccaagcgg ggaaggatga gaagcttcga
1081 ccaaggttcg acttgagcgc cagcgagaga gcgttgccgc aggcaacgac ccgaagggcg
1141 aagcgcgcag cgctgagtaa tccttccccc accaccatcg cttccttaaa taatcaaaac
1201 ctgttactcc gagctaattt tgcccgatgg caccacgttt accgggccta caatatcctc
1261 ccctgtaggt cggataagcg aagcgcatcc gacaactcac cccgcaccgt gcccataaaa
1321 aaaccccgcc gaagcagggt ctatgtgaac taaaattcgc ttagtgacga gcgcgcacca
1381 gctggtactt acgtgtcaga tactccaccg gtacgctcca gatgtgtatc agacgcgaga
1441 acgggaacag caggaataac gtcatcccca gcaccaggtg cagacggaag ataaacgcca
1501 caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac caccgactgc gcccagccaa
1561 ccagtttcat catctcgcta ccgtccatat gctgagcgga gaacggaatg gtcaacaggc
1621 ccagcgcgca ctggataacg agcagcgaca ggatc
//