GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTRPA 1404 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli trpA gene (codes for tryptophan synthetase alpha-SU).
ACCESSION V00364
KEYWORDS synthetase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 878)
AUTHORS Nichols,B.P. and Yanofsky,C.
TITLE Nucleotide sequences of trpA of Salmonella typhimurium
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 76, 5244-5248 (1979)
STANDARD full automatic
REFERENCE 2 (bases 775 to 1404)
AUTHORS Wu,A.M., Chapman,A.B., Platt,T., Guarente,L.P. and Beckwith,J.
TITLE Deletions of distal sequence affect termination of transcription at
the end of the tryptophan operon in E. coli
JOURNAL Cell 19, 829-836 (1980)
STANDARD full automatic
REFERENCE 3 (bases 1 to 878)
AUTHORS Schneider,W.P., Nichols,B.P. and Yanofsky,C.
TITLE Procedure for production of hybrid genes and proteins and its use
in assessing significance of amino acid differences in homologous
tryptophan synthetase alpha polypeptides
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78, 2169-2173 (1981)
STANDARD full automatic
COMMENT SWISS-PROT; P00928; TRPA$ECOLI.
See also <ECTRPX> for the complete trp operon (with slight
differences from this sequence).
Homologous to S. typhimurium trpA gene (199 differences).
From EMBL entry ECTRPA; dated 01-AUG-1990.
FEATURES Location/Qualifiers
mRNA <1..911
/note="messenger RNA"
CDS <1..69
/note="reading frame trpB"
/codon_start=1
conflict 18..18
/note="A is C in [1]"
/citation=[1]
CDS 72..875
/note="reading frame trpA"
/codon_start=72
BASE COUNT 369 a 355 c 324 g 352 t 4 others
ORIGIN
1 aacctttccg gtcgcggaga taaagacatc ttcaccgttc acgatatttt gaaagcacga
61 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
121 gcgcattcgt tcctttcgtc acgctcggtg atccgggcat tgagcagtca ttgaaaatta
181 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggtatcccc ttctccgacc
241 cactggcgga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
301 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
361 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
421 agtgcgaaaa agtcggcgtc gattcggtgc tggttgccga tgtgccagtt gaagagtccg
481 cgcccttccg ccaggccgcg ttgcgtcaca acgtcgcacc tatcttcatc tgcccgccaa
541 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
601 tgtcacgagc aggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
661 ttgcgaagct gaaagagtac aacgctgcac ctccattgca gggatttggt atttccgccc
721 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcggcca
781 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
841 tttttgtaca accgatgaaa gcggcgacgc gcagttaatc ccacagccgc cagttccgct
901 ggcggcattt taactttctt taatgaagcc ggaaaaatcc taaattcatt taatatttat
961 ctttttaccg tttcgcttac cccggtcgat cgtyractta cgtcattttt ccgcccaaca
1021 gtaatataaa caaacaaatt aaacccgcaa cataacacca gtaaaatcaa taattttctc
1081 taagtcactt attcctcagg taattcttaa tatatccaga atgttcctca aaatatattt
1141 tccctctatc ttctcgttgc gcttaatttg actaattctc attagcgact aattttaatg
1201 agtgtcgaca cacaacactc atattaatga aacaatgcaa cgcaacggga gaaataacat
1261 ggccgaacat cgtggtggtt caggaaattt cgccgaagac cgtgagaagg catccgacgc
1321 agccgtaaag gcggtcagca tagcggcggt aattttaaaa atgatcgcaa cgcgcatctg
1381 aagcgggtaa aaaaggcggt yrac
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