GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTYRU 717 bp ds-DNA BCT 27-MAY-1991
DEFINITION E.coli tyr Ucluster and tufB gene 5'end
ACCESSION X04181
KEYWORDS elongation factor; elongation factor tu; transfer RNA;
transfer RNA-Gly; transfer RNA-Thr; transfer RNA-Tyr; tufB gene;
tyrU gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 717)
AUTHORS Hudson,L., Rossi,J. and Landy,A.
TITLE Dual function transcripts specifying tRNA and mRNA
JOURNAL Nature 294, 422-427 (1981)
STANDARD full automatic
COMMENT See also X04176 for overlapping sequence (some differences)
From EMBL entry ECTYRU; dated 19-SEP-1987.
FEATURES Location/Qualifiers
repeat_unit 53..66
/note="imp.inverted repeat A"
misc_feature 54..78
/note="A+T rich region"
repeat_unit 71..83
/note="imp.inverted repeat A'"
promoter 81..86
/note="-35 sequence (tyrU)"
repeat_unit 90..100
/note="imp.inverted repeat B"
promoter 104..110
/note="-10 region box (tyrU)"
repeat_unit 105..114
/note="imp.inverted repeat B'"
tRNA 117..>116
/note="tRNA-Thr(4) - tRNA-Tyr(2) dimer (about 200 bases)"
misc_feature 117..117
/note="transcription start site (tyrU cluster)"
tRNA 124..199
/note="tRNA-Thr (4)"
repeat_unit 189..205
/note="imp.inverted repeat C'"
tRNA 208..292
/note="tRNA-Tyr (2)"
repeat_unit 295..305
/note="imp.inverted repeat D"
repeat_unit 310..320
/note="imp.inverted repeat D'"
tRNA 398..567
/note="tRNA-Gly(2)-tRNA-Thr(3) dimer"
tRNA 408..482
/note="tRNA-Gly(2)"
tRNA 489..564
/note="tRNA-Thr(3)"
misc_feature 606..615
/note="sequence pot.involved in processing"
misc_signal 607..612
/note="pot.termination sequence"
repeat_unit 611..627
/note="imp.inverted repeat E"
repeat_unit 612..620
/note="imp.inverted repeat F"
repeat_unit 624..632
/note="imp.inverted repeat F'"
repeat_unit 638..654
/note="imp.inverted repeat E'"
promoter 640..646
/note="pot.promotor (tuf B)"
RBS 667..672
/note="pot.ribosome binding site"
CDS 688..717
/note="elongation factor Tu (Ef-Tu) (tuf B gene) (AA
1-13)"
/codon_start=688
BASE COUNT 170 a 179 c 172 g 196 t
ORIGIN
1 ctaatctgcc ctccgttcgg ctgtttcttc atcgtgtcgc ataaaatgtg accaataaaa
61 caaattatgc aattttttag ttgcatgaac tcgcatgtct ccatagaatg cgcgctactt
121 gatgccgact tagctcagta ggtagagcaa ctgacttgta atcagtaggt caccagttcg
181 attccggtag tcggcaccat caagtccggt ggggttcccg agcggccaaa gggagcagac
241 tgtaaatctg ccgtcacaga cttcgaaggt tcgaatcctt cccccaccac caatttcggc
301 cacgcgatgg cgtagccgag acgataagtt cgcttaccgg ctcgaataaa gagagcttct
361 ctcgatattc agtgcagaat gaaaatcagg tagccgagtt ccaggatgcg ggcatcgtat
421 aatggctatt acctcagcct tccaagctga tgatgcgggt tcgattcccg ctgcccgctc
481 caagatgtgc tgatatagct cagttggtag agcgcaccct tggtaagggt gaggtcggca
541 gttcgaatct gcctatcagc accacttctt ttctcctccc tgttttttcc ttctgtttat
601 tgcattcaac aagtcgggca tgttgcctgg ttgatgtggt gatgtcaccg atttatccgt
661 gtcttagagg gacaatcgat gtctaaagaa aagtttgaac gtacaaaacc gcacgtt
//