GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOWITAG 1560 bp ds-DNA BCT 27-MAY-1991
DEFINITION E.coli witA gene
ACCESSION X53027
KEYWORDS transporter protein; witA gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1560)
AUTHORS Seol,W. and Satkin,A.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Seol,W. and Shatkin,A.J.
TITLE A new gene located between pss and rrnG on the Escherichia coli
chromosome
JOURNAL J. Bacteriol. 172, 4745-4745 (1990)
STANDARD full staff_review
REFERENCE 3 (sites)
AUTHORS Seol,W. and Shatkin,A.J.
TITLE Escherichia coli kgtP encodes an alpha-ketoglutarate transporter
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 88, 3802-3806 (1991)
STANDARD full staff_review
COMMENT *source: strain=K12; **map: map position=56.5 minutes;
From EMBL entry ECWITAG; dated 29-OCT-1990.
FEATURES Location/Qualifiers
promoter 75..80
/note="Pribnow box"
RBS 137..141
/note="ribosome binding site"
CDS 152..1450
/note="witA gene product"
/codon_start=152
BASE COUNT 348 a 354 c 399 g 459 t
ORIGIN
1 cttaagctcc tcaataacca ttttcctgct aactaaattc atggttaagg ttgcataatg
61 atatgcaaca aatgtataat atttccttta caaaaaaaat aaacaaaagc gaccgacaaa
121 agcatcggat tacggcagga gacataatgg catggctgaa agtactgtaa cggcagacag
181 caaactgaca agtagtgata ctcgtcgccg catttgggcg attgtggggg cctcttcagg
241 taatctggtc gagtggttcg atttctatgt ctactcgttc tgttcactct actttgccca
301 catcttcttc ccttccggga acacgacgac tcaactacta caaacagcag gtgtttttgc
361 tgcgggattc ctgatgcgcc caataggcgg ttggctattt ggccgcatag ccgataaaca
421 tggtcgcaaa aaatcgatgc tgttatcggt gtgtatgatg tgtttcggat cgctggttat
481 cgcctgcctc ccaggttatg aaactatagg tacgtgggct ccggcattat tgcttctcgc
541 tcgtttattt cagggattat ctgttggcgg agaatatggc accagcgcca cctatatgag
601 tgaagttgcc gttgaagggc gcaaaggttt ttacgcatca tttcagtatg tgacgttgat
661 cggcggacaa ctgctagccc tactggttgt cgtggtttta caacacacca tggaagacgc
721 tgcactcaga gagtggggat ggcgtattcc tttcgcgtta ggagctgtgt tagctgttgt
781 ggcgttgtgg ttacgtcgtc agttagatga aacttcgcaa caagaaacgc gcgctttaaa
841 agaagctgga tctctgaaag gattatggcg caatcgccgt gcattcatca tggttctcgg
901 ttttaccgct gcgggctccc tttgtttcta taccttcact acttatatgc agaagtatct
961 ggtaaatact gcgggaatgc atgccaacgt ggcgagtggc attatgactg ccgcattgtt
1021 tgtattcatg cttattcaac cactcattgg cgcgctgtcg gataagattg gtcgccgtac
1081 ctcaatgtta tgtttcggtt cgctggcagc catttttacc gttcctattc tctcagcatt
1141 gcaaaacgtt tcctcgcctt atgccgcttt tggtctggtg atgtgtgccc tgctgatagt
1201 gagtttttat acatcaatca gtggaatact gaaggctgag atgttcccgg cacaggttcg
1261 cgcattaggc gttggtctgt catatgcggt cgctaatgct atatttggtg gttcggcgga
1321 gtacgtagcg ttgtcgctga aatcaatagg aatggaaaca gccttcttct ggtatgtgac
1381 cttgatggcc gtggtggcgt ttctggtttc tttgatgcta catcgcaaag ggaaggggat
1441 gcgtctttag tgacgggtca gttgccagac ggtatagccg gtgcttgcac cggcgacatc
1501 ccaggccaaa tccttccagc tccagccgct cccttcgggg cggctatccc aaagctcttt
//