GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS FVBNYLBB 1455 bp ds-DNA BCT 27-MAY-1991
DEFINITION Flavobacterium sp. K172 pOAD2 RS-IIB repeat with nylB' gene coding
for enzyme EII' degrading nylon oligomers
ACCESSION X02864
KEYWORDS nylon; plasmid.
SOURCE Flavobacterium sp. DNA.
ORGANISM Flavobacterium sp.
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE 1 (bases 1 to 1455)
AUTHORS Okada,H., Negoro,S., Kimura,H. and Nakamura,S.
TITLE Evolutionary adaptation of plasmid-encoded enzymes for degrading
nylon obligomers
JOURNAL Nature 306, 203-206 (1983)
STANDARD full automatic
COMMENT SWISS-PROT; P07062; NYLC$FLASP.
From EMBL entry FSNYLBB; dated 28-JAN-1986.
FEATURES Location/Qualifiers
misc_feature 1..1455
/note="RS-II B repeated sequence"
RBS 211..214
/note="put. rRNA binding site"
CDS 222..1397
/note="EII' (aa 1-392)"
/codon_start=222
BASE COUNT 224 a 555 c 464 g 212 t
ORIGIN
1 cccgggggcc aagtactggt acaccggtgg cttcagcatt cccggcctcg ccgcggggat
61 catcggcggc gcagccgcct tcttcctcac caccgacttc tggagcggcg cgatcgccgg
121 gccatgggct acatcgatct ctccgctccc gttgcgatga tcgccagcgg cggactgtac
181 taccacttca cccgccgcgg ccacaccttc ggagacgctc gatgaacacg cccaccaccg
241 gctcgcaccc cgcccgatac cccagcgccg ccgcgggtga accgacgctc gacagctggc
301 aggagccccc gcacaaccgc tgggccttcg ctcacctcgg cgagatggtg ccctcggcag
361 cggtctcgcg gcgcccggtg aacgcgcccg gtcatgccct cgcgcggctc ggcgcgatcg
421 cggcgcagct tcccgacctc gaacagcgcc tcgaacagac ctacaccgac gccttcctcg
481 tgctgcgcgg aacggaggtc gtggccgagt actaccgcgc cggcttcgcg cccgacgacc
541 gtcacctgct gatgagcgtc tcgaagtcgc tgtgcggcac ggtcgtcgga gcactggtcg
601 acgaggggcg catcgatccc gcgcagcccg tcaccgagta tgtacccgag ctcgccggct
661 ccgtctacga cgggccctcc gtgctgcagg tgctcgacat gcagatctcg atcgactaca
721 acgaggacta cgtcgatccc gcatccgagg tgcagaccca cggtcgctcg gccggctggc
781 gcacgcgcgc cacgggcgac ccggccgaca cctacgagtt cctcaccacc ctccgcggcg
841 acggcagcac cggcgagttc cagtactgct cggcgaacac cgacgtactc gcctggatcg
901 tcgaacgggt caccggtctg cgctacgtcg aagcgctctc cacgtacctg tgggcgaagc
961 tcgacgccga ccgcgacgct accatcaccg tcgacacgac gggtttcggc ttcgcgcacg
1021 gcggcgtctc ctgcacggcg cgggatctcg cgcgcgtggg gcgcatgatg ctcgacggcg
1081 gcgtcgcccc cggtggccgc gtggtctccg aggactgggt gcgtcgcgta ctcgccggcg
1141 gatcgcacga ggcgatgacc gacaagggct tcacgaacac cttccccgac ggcagctaca
1201 cccgccagtg gtggtgcacg ggcaacgagc gcggcaacgt gagcggcatc ggcatccacg
1261 ggcagaatct gtggctcgac ccgctcaccg actcggtgat cgtcaagctg tcgtcgtggc
1321 ccgatcccga caccgagcac tggcaccggc tgcagaacgg gatcctgctc gacgtcagcc
1381 gcgcgctcga cgcagtgtag ccacggtcgc tccgctccgc cgggcctccc ccgccggtag
1441 agcgaggagc gcagc
//