GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HEIHINCII 2825 bp ds-DNA BCT 27-MAY-1991
DEFINITION Haemophilus influenzae genes for HincII restriction-modification
system (HincII methyltransferase (EC 2.1.1.72) and HincII
endonuclease (EC 3.1.21.4))
ACCESSION X52124
KEYWORDS HincII endonuclease; HincII methyltransferase; endonuclease;
methyltransferase; nuclease; restriction modification enzyme;
transferase.
SOURCE Haemophilus influenzae DNA.
ORGANISM Haemophilus influenzae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Pasteurellaceae.
REFERENCE 1 (bases 1 to 2825)
AUTHORS Ito,H.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2825)
AUTHORS Ito,H., Sadaoka,A., Kotani,H., Hiraoka,N. and Nakamura,T.
TITLE Cloning, nucleotide sequence, and expression of the HincII
restriction-modification system
JOURNAL Nucleic Acids Res. 18, 3903-3911 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17743; T2H2$HAEIN. SWISS-PROT; P17744; MTH2$HAEIN.
*source: strain=Rc; library=pBR322; clone=pAH2 plasmid;
Data kindly reviewed (10-SEP-1990) by Ito H.
From EMBL entry HIHINCII; dated 24-SEP-1990.
FEATURES Location/Qualifiers
misc_feature 462..491
/note="stem loop structure"
CDS 516..2021
/note="HincII methyltransferase (AA 1-502)"
/codon_start=516
CDS 2021..2794
/note="HincII endonuclease (AA 1-258)"
/codon_start=2021
BASE COUNT 1045 a 340 c 487 g 953 t
ORIGIN
1 aagcttacga ctctgaattc cttgagggag aacaagtcga agtggctcgc gtgaaaattg
61 ttaatcgcca acgtgaagca gaaggcaaac caccagttga atttgaacgt gaattattgg
121 gtatcaccaa agcgtctctt gcgactgaat ccttcatctc tgccgcatcg ttccaagaaa
181 ctactcgtgt tcttaccgaa gcagcagtgg caggtaaacg tgatgaatta cgtggcttga
241 aagagaacgt aatagtaggt cgattaattc ctgcgggtac aggttttgcg tatcatcaaa
301 atcgtcacaa acatcgctta gttgatgatg tcgtagcgaa attatctgaa gaagatgaag
361 ccgtatcgct gatgagtgtc ataacggcag atgatgcaac gcaaaatctt gcaacattgt
421 taaattctga aatagaagat taatatttgt atcttatgtt taaaccctca gaatgagagt
481 tctgagggtt tttattagaa aaataggata aaaatatgga tgaatcaaag aaaatatcac
541 taggtcagtt ttttactcct acacatattg ttaaatatat gattggacta atgacgaaaa
601 ataaaaatgc tagtatttta gaacctagta gtgggaatgg tgtatttcta gatagtttaa
661 ttcaattagg ttatacaaat ttaacatcgt atgaaataga tggtgatatt atttctcatc
721 catttgtaat taactcaagt tttataacat catatgataa gcctcaatat gattcgatca
781 ttggaaatcc tccttatgtt cgttggaaaa acctaagtga gcttcagaag aaagagctta
841 aagataattc tatatggaaa atgtattgta atagtctttg tgattatttc tatattttta
901 ttattaaatc tatacttcaa ttaaaagttg gaggagaatt aatatttatt tgccctgatt
961 attttttttc cactaagaac gctgagggtt taaggaaatt tttgataaat aatggaagtt
1021 ttgaaaaaat tatattattt aatgaaagta aagtatttca tggagtttct tcttcagttg
1081 ttatttttaa atatataaaa ggaaaaaata ttgataatat aaatattatt aatatagatt
1141 caaaaagccc aataaaatct gaggatatag aatctttagg tgaatcatat tatattccaa
1201 gattttctag ctcagatgtt tgggttactt cgcccaatca tattaaagtt gcattagata
1261 aatttgagag ttactgcaaa actatcaaaa aagttcagca aaaatcatta tttgatgatc
1321 ttagttttct agataaggct tttcagatga atgatataaa ttattcagaa ttagaattgc
1381 ttaattcaat ttgtgtagct aaagctaaac acttagacgc attttgtttc tctggataca
1441 ccagatacaa gttaatatta gatgataata atgaggataa attaatcact tattttccaa
1501 atttctttta tgagtttaat aattataaag attatctatt aaaaagatat agttataata
1561 aatatttgcc ttattgggaa gtggcttttc ttagaaattt ttctctattc agcaagaatg
1621 agaagaaaat atttgtgccg tgcaaagaaa gaatttcaaa aaaaagtaat tttagattta
1681 gcttagttga tgaatttata tatccaacac aggatgttac tgcattatat aagaaagaaa
1741 atgtaaagga gagtattgag tatattacag catatttaaa ctctaaagct gtgtttttat
1801 ggatgaagta taagggtgtt gtcaaaggta atgtagttga attttcagaa aaaccattaa
1861 caaatattcc ttttagaaga atagattggc aattaaaaag cgaaaaaaaa attcatgatg
1921 atataactaa tttagtaaga aaatatttat caaacaaaga attttcaatt ttacatgaga
1981 ttaacttaaa tttagaaaaa ttaggtataa aggtggaaat atgagtttca taaaacctat
2041 ttatcaggat attaattcaa tattaatcgg gcaaaaagtg aaacgtccta aatcaggtac
2101 tctgtcaggt catgctgcag gggaaccatt tgaaaaatta gtatataagt ttttgaaaga
2161 aaacctgtca gatttaacat ttaagcaata tgaatatctt aatgatttat ttatgaagaa
2221 ccctgcgata attggacatg aagctagata taaattattt aattctccaa cattgctttt
2281 tttgttaagt agaggtaaag ctgcaactga aaattggagc atagaaaatt tatttgagga
2341 gaaacaaaat gatactgcag atattttatt agtaaaggat cagttctatg aattgttaga
2401 tgtcaaaaga agaaatatta gtaaatcagc tcaagcaccc aatattattt cagcatataa
2461 attagctcag acgtgtgcaa aaatgattga taataaagaa tttgatttat ttgatattaa
2521 ttatttagaa gtagactcgg aacttaatgg tgaagatcta gtttgtgtat ctacttcttt
2581 tgctgaacta tttaaatctg aacctagtga actatatatt aactgggctg cagctatgca
2641 aattcagttt catgtaagag atttagatca ggggtttaat ggaactagag aagaatgggc
2701 aaaatcttat ctaaaacatt ttgttactca agcagagcaa agagccatat ctatgataga
2761 taagtttgtt aagccattta agaaatatat actttagttt caagttcaaa agatattata
2821 agctt
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