GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTN1721 490 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli transposon Tn1721 tnpR gene fragment for transposition
resolvase
ACCESSION X01299
KEYWORDS resolvase binding site; transposition resolvase; transposon.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 490)
AUTHORS Diver,W.P., Grinsted,J., Fritzinger,D.C., Brown,N.L.,
Altenbuchner,J., Rogowsky,P. and Schmitt,R.
TITLE DNA sequences of and complementation by the tnpR genes of Tn21,
Tn501 and Tn1721
JOURNAL Mol. Gen. Genet. 191, 189-193 (1983)
STANDARD full automatic
REFERENCE 2 (bases 488 to 488)
AUTHORS Schmitt,R.
JOURNAL Unpublished (1985)
STANDARD full automatic
REFERENCE 3 (sites)
AUTHORS Rogowsky,P., Halford,S.E. and Schmitt,R.
TITLE Definition of three resolvase binding sites at the res loci of Tn21
and Tn1721
JOURNAL EMBO J. 4, 2135-2141 (1985)
STANDARD full staff_review
COMMENT Data kindly reviewed (13-NOV-1985) by Schmitt R.
From EMBL entry ISTN1721; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 221..258
/note="put. resolvase binding site I(ref[2])"
misc_feature 239..240
/note="recombination cross over point(ref[2]"
misc_feature 272..313
/note="put. resolvase binding site II(ref[2]"
promoter 305..310
/note="pot. -35 region"
promoter 327..332
/note="pot. -10 region"
precursor_RNA 341..>490
/note="put. primary transcript"
RBS 401..405
/note="put. Shine-Dalgarno region"
CDS 410..>490
/note="transposition resolvase"
/codon_start=410
old_sequence 488..488
/note="g was t in [1]"
/citation=[1]
BASE COUNT 127 a 110 c 129 g 124 t
ORIGIN
1 ttaacgtacg tgatatggct ttgaggggag gtagtcgaaa gaatattggc atcttttggg
61 agttctaagt ttcgacccgt cactggtaaa ttctggcgca taagaacctc aagggttggc
121 tgttttattt tattgttttc ggcattaagc ccaatttctt gagcgttacg ataaagctag
181 catggaaacg ataggtgcaa gcaagttaag ggttgcatcg cgcatgtcaa tctaggctat
241 accctaactt gatgtcaggc agggccgcgc cgcttcgtca gaatagagtc tgctttccca
301 ttttttgaca catgcccgcg aaggttatag atttcagcct gacagaaatg ggctttgagg
361 cacaacggaa cagaaagtgc acttaagccg ccttcaacca aggagacatc gtgcaggggc
421 accgcatcgg ctacgttcgg gtcagcagct ttgaccagaa cccggaacgc cagctggaac
481 aaacccaggt
//