GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTN21A 1115 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli transposon Tn21 tnpR gene for transposition resolvase
ACCESSION X01298
KEYWORDS resolvase binding site; transposition resolvase; transposon.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1115)
AUTHORS Diver,W.P., Grinsted,J., Fritzinger,D.C., Brown,N.L.,
Altenbuchner,J., Rogowsky,P. and Schmitt,R.
TITLE DNA sequences of and complementation by the tnpR genes of Tn21,
Tn501 and Tn1721
JOURNAL Mol. Gen. Genet. 191, 189-193 (1983)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Rogowsky,P., Halford,S.E. and Schmitt,R.
TITLE Definition of three resolvase binding sites at the res loci of Tn21
and Tn1721
JOURNAL EMBO J. 4, 2135-2141 (1985)
STANDARD full staff_review
COMMENT SWISS-PROT; P04130; TNP1$ECOLI.
From EMBL entry ISTN21A; dated 07-NOV-1985.
FEATURES Location/Qualifiers
misc_feature 369..405
/note="put. resolvase binding site I [2]"
misc_feature 388..389
/note="recombination cross-over point [2]"
misc_feature 421..463
/note="put. resolvase binding site II [2]"
promoter 454..459
/note="pot. -35 region"
misc_feature 468..497
/note="put. resolvase binding site III [2]"
promoter 477..482
/note="pot. -10 region"
RBS 500..504
/note="put. Shine-Dalgarno region"
CDS 510..1067
/note="transposition resolvase"
/codon_start=510
CDS 1073..>1115
/note="put. tnpA protein (transposase)"
/codon_start=1073
BASE COUNT 245 a 306 c 338 g 226 t
ORIGIN
1 gacgttcagt gcagccgtct tctgaaaacg acaatggagg tggtagccga gggtgtggaa
61 acacccgact gccttgcgtg gttgcggcag gcgggttgcg acacggtgca gggtttcctg
121 ttcgccaggc cgatgccggc ggcggccttc gtcggcttcg tcaaccaatg gaggaacacc
181 accatgaacg ccaatgaacc gagcaccagt tgctgcgtgt gctgcaagga aatcccgctc
241 gatgccgcct tcacgccgga aggggccgag tacgtggagc atttctgcgg gctggagtgc
301 tatcagcgct tccaggcgcg ggccagcact gcgaccgaaa ccagcgtcaa accggacgct
361 tgtgattcgc cgccgtcagg ttgaggcata ccctaacctg atgtcagatg ccatgtgtaa
421 attgcgtcag gataggattg aattttgaat ttattgacat atctcgttga aggtcataga
481 gtcttccctg acattttgca gggaattcca tgactggaca gcgcattggg tatatcaggg
541 tcagcacctt cgaccagaac ccggaacggc aactggaagg cgtcaaggtt gatcgcgctt
601 ttagcgacaa ggcatccggc aaggatgtca agcgtccgca actggaagcg ctgataagct
661 tcgcccgcac cggcgacacc gtggtggtgc atagcatgga tcgcctggcg cgcaatctcg
721 atgatttgcg ccggatcgtg caaacgctga cacaacgcgg cgtgcatatc gaattcgtca
781 aggaacacct cagttttact ggcgaagact ctccgatggc gaacctgatg ctctcggtga
841 tgggcgcgtt cgccgagttc gagcgcgccc tgatccgcga gcgtcagcgc gagggtattg
901 cgctcgccaa gcaacgcggg gcttaccgtg gcaggaagaa atccctgtcg tctgagcgta
961 ttgccgaact gcgccaacgt gtcgaggctg gcgagcaaaa gaccaagctt gctcgtgaat
1021 tcggaatcag tcgcgaaacc ctgtatcaat acttgagaac ggatcagtaa atatgccacg
1081 tcgttccatc ctgtccgccg ccgagcggga aagcc
//