GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTN3X 4957 bp ds-DNA BCT 27-MAY-1991
DEFINITION Transposon Tn3 (E. coli).
ACCESSION V00613
KEYWORDS drug resistance gene; insertion sequence.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 3738 to 4844)
AUTHORS Sutcliffe,J.G.
TITLE Nucleotide sequence of the ampicillin resistance gene of
Escherichia coli plasmid pBR322
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 75, 3737-3741 (1978)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4957)
AUTHORS Heffron,F., Mccarthy,B.J., Ohtsubo,H. and Ohtsubo,E.
TITLE DNA sequence analysis of the transposon Tn3: Three genes and three
sites involved in transposition of Tn3
JOURNAL Cell 18, 1153-1163 (1979)
STANDARD full automatic
COMMENT SWISS-PROT; P00810; BLAT$ECOLI. SWISS-PROT; P03008; TRA1$ECOLI.
SWISS-PROT; P03011; TRAR$ECOLI.
From EMBL entry ISTN3X; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 1..38
/note="inverted repeat"
CDS complement(37..3081)
/note="reading frame (transposase)"
/codon_start=3081
CDS 3210..3764
/note="reading frame (repressor)"
/codon_start=3210
CDS 3950..4807
/note="reading frame (beta-lactamase)"
/codon_start=3950
misc_feature 4920..4957
/note="inverted repeat"
BASE COUNT 1249 a 1238 c 1234 g 1236 t
ORIGIN
1 ggggtctgac gctcagtgga acgaaaactc acgttaagca acgttttctg cctctgacgc
61 ctcttttaat ggtctcagat gacctttggt caccagttct gccagcgtga aggaataatg
121 gccgagcata ttgatatgtc cgtggcaaag cggggagagg cgtgcgatat cttcatcatt
181 cagtgtttca ccctgcgccc ggagatgatc cagagctgcc tgcatataaa tagtgttcca
241 taacacgacg gcgttagtga ccagccccag agctcccaac tgatcttcct gaccgtcggt
301 atatcgtttt cttatctcac ctttttgacc gtgacagatg gctctggcaa ctgcatgacg
361 gctttctccc cgattaagct gggtcagaat gcgccggcgg taatcttcat catcaatata
421 attaagcaga tacagcgttt tgttgatgcg ccccacttca atgattgcct gagtcagtcc
481 ggagggacgt tcacttttca gcaatgaacg gaccagcact gaggcctgta ctttgcccag
541 cttcagggag cctgcggtcc ggatcatttc gtcccactga aggactattt ttcggggatc
601 tgattgccct ctggcaatat cattcagcac gccatagtcg gcatcatggt ccattcgcca
661 gaaaaccgaa gcaccggcat cagccaggcg tggagaaaac tggtatccca gcagccagaa
721 aaggccaaag acaagatcgc tggcacctgc cgtatcggtc ataatttcgg ttggattcag
781 cccggtctcc tgttccagaa ggccttccag cacaaagata gagtccctca gcgtccccgg
841 tataacgatg ccatgaaagc catgaaagcc ggaatactga tcggacacaa agttgtacca
901 ggtgatccct ctgttattac caaagtattt gcggttcggt ccggcattga ttgttctgac
961 tggcgtaaca aagcgcattc catctgcaga tgccacttct cctccacccc atatctgtgc
1021 cagtggcagc gttgcctgaa aatcaaccag tctggcatta gcgctggtga tagtttcagc
1081 ccgcagatag ttcgcttttg tccagttcag ccggtgtcgg gtcagtgcag gaacatttga
1141 tctgatcagt ggttccagac cgatattgca ggcttcagcc atcagcacgg cgctgatgct
1201 gacgggcaga tcatcaactc tggcactggc ttcactggca tggaaaaact catcagcaaa
1261 tccggtatgg gcgttaattt cgagcagcaa ctccgttaaa tccaccggag ggagcagatc
1321 actgatcatt ttgctcagtc gtttcagact gtccggctca tcaagactgg cgaggggaga
1381 aattgtcaac cggggcttcg ggccagaaac atcgagttcg acagcctcat tttcgccaag
1441 acgtgcagca acctgtctgt aacgactatc aagctgatgg cccagagatt ttattgcttc
1501 ctgcgggtct gtcgggtgcc ccaaagaacg ataaacctta atccgatttg cctgccagtc
1561 agcaccctgt agtaatcttg cacgaggatc tccccaccgg ttactgccgg taacgtagac
1621 atccctccgt ctcagactat cctgcagttt actgagaaag cagagcgtgt atccccttcg
1681 ggtgatatgt ttttccttgt taatcaccag ccgtttccat gactgactga tgatttccgt
1741 tggtgcgtcg tcaaaaaact gccgccgtga gctgaactcc cggctgaggt agtcacaggc
1801 attcagagtg gtaaccccgg caggtgcgga tgaaaattta acggtattca gcagatgggg
1861 caggaaacga cgaacgcgcc cgtactgctc caccatttcg tcatgaaaat tatcgtctga
1921 gggccgggca atttcacgga caagcgtgat gatttcagcc agcttttgcc tagggatgta
1981 gctgaacacc tcagcacgaa tcgattcgtc cggtgtttct tctttcagca agtacgaaca
2041 tgcgctggcg agcgccaatg cagatttatc cagatccttc agcgagcgga gccgtttttt
2101 ctgcccaatc tttctggcgt cacggatgat aacggccagc atggcgtcca gaacgtccag
2161 tgcatcatcc agcgccagcg tttcccatgc aaggacaaag gcaaccagaa ccgccatcct
2221 tttctgcggt gacatcctgg caatattgaa caccgaagtc ataccagcat aacgtgcgag
2281 atttttcagg cgcacagccg ggagtgtact caggttttca gcatgcaggc caaaatcgtt
2341 cagagttttc cagcgttcaa ttgcttcatt aaacgccgga ccactgatgg tcacagggcc
2401 ttttttcagt gattccagta aagacaggcg gctgcaatca gttggcccca gcagcatctc
2461 cagctgtgaa cgctgttcgg ctgacggtat cagtgccagt ttgttccaca ggcgcaacgt
2521 cgccttttcc cttacctctg aaatcaaccg ggtcagcgtg gtggctccgg ggagaataat
2581 acgatgttgc ataagccacc ctgtcgccag gtcgaaaagc aggccaggac gttcgttgct
2641 tatccagctc cgggtatata aaagacgggt aaggcgaaat gtccagggcc aggcaaattc
2701 acgatactga tagtgctgac gtatcagcgc tgcatgctca cggcgggtat tttccctctg
2761 accgtattct gcaagaacgg tgatatcacg aatcccgagc tgtctggcgg taaaatgccg
2821 gacgccggaa ggaatatgat tcatgtcagt aagaaaagtg cccagaaaac gcacacaccc
2881 gatttgcagg gcaataccaa gtcgattgtg atcaccccgg cttttcccga taaattcttt
2941 atccgcttca tcaagatgaa aataacgcgc cagctgaagt tcatcgggtt cgccagtgaa
3001 cctgccataa ctctcaacct gctcagtggt caaaaaatca acgggcatat cggcctccct
3061 gcctgacggc ttttttaaca caactgcaac cgttcgaaat attataaatt atcagacata
3121 gtaaaacggc ttcgtttgag tgtccattaa atcgtcattt tggcataata gacacatcgt
3181 gtctgatatt cgatttaagg tacattttta tgcgaatttt tggttatgcg cgggtctcaa
3241 ccagccagca gtccctcgat attcagatca gagcgctcaa agatgcaggg gtaaaagcta
3301 accgcatctt taccgacaag gcatccggca gttcaacaga tcgggaaggg ctggatttgc
3361 tgaggatgaa ggtggaggaa ggtgatgtca ttctggtgaa gaagctcgac cgtcttggcc
3421 gcgacaccgc cgacatgatc caactgataa aagagtttga tgctcagggt gtagcggttc
3481 ggtttattga cgacgggatc agtaccgacg gtgatatggg gcaaatggtg gtcaccatcc
3541 tgtcggctgt ggcacaggct gaacgccgga ggatcctaga gcgcacgaat gagggccgac
3601 aggaagcaaa gctgaaagga atcaaatttg gccgcaggcg taccgtggac aggaacgtcg
3661 tgctgacgct tcatcagaag ggcactggtg caacggaaat tgctcatcag ctcagtattg
3721 cccgctccac ggtttataaa attcttgaag acgaaagggc ctcgtgatac gcctattttt
3781 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
3841 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
3901 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
3961 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
4021 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
4081 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
4141 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
4201 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
4261 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
4321 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
4381 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
4441 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
4501 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
4561 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
4621 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
4681 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
4741 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
4801 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
4861 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
4921 ttaacgtgag ttttcgttcc actgagcgtc agacccc
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