GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STATN554 6691 bp ds-DNA BCT 27-MAY-1991
DEFINITION Staphylococcus aureus transposon Tn554
ACCESSION X03216
KEYWORDS erythromycin resistance; methylase; overlapping genes;
spectinomycin resistance; transposase; transposon;
unidentified reading frame.
SOURCE Staphylococcus aureus DNA.
ORGANISM Staphylococcus aureus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 1 to 6691)
AUTHORS Murphy,E., Huwyler,L. and do,C.d.F.B.M.
TITLE Transposon Tn554: complete nucleotide sequence and isolation of
transposition-defective and antibiotic-sensitive mutants
JOURNAL EMBO J. 4, 3357-3365 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P04827; S3AD$STAAU. SWISS-PROT; P06696; TNPA$STAAU.
SWISS-PROT; P06697; TNPB$STAAU. SWISS-PROT; P06698; TNPC$STAAU.
SWISS-PROT; P06699; MLS1$STAAU.
From EMBL entry ISTN554; dated 02-JUL-1986.
FEATURES Location/Qualifiers
RBS 118..126
/note="pot. Shine-Dalgarno sequence"
CDS 134..1216
/note="tnpA protein (aa 1-361)"
/codon_start=134
RBS 1201..1207
/note="pot. Shine-Dalgarno sequence"
CDS 1216..3105
/note="pot. tnpB protein (aa 1-630)"
/codon_start=1216
RBS 3099..3109
/note="pot. Shine-Dalgarno sequence"
CDS 3115..3489
/note="pot. tnpC protein (aa 1-125)"
/codon_start=3115
RBS 3629..3638
/note="pot. Shine-Dalgarno sequence"
CDS 3643..4422
/note="adenyltransferase AAD9 (spc) (aa 1-260)"
/codon_start=3643
CDS complement(4554..5282)
/note="S-adenosyl-methionine dependent methylase (ermA)
(aa 1-243)"
/codon_start=5282
RBS complement(5292..5300)
/note="pot. Shine-Dalgarno sequence"
CDS complement(5343..5399)
/note="peptide L (aa 1-19)"
/codon_start=5399
RBS complement(5408..5415)
/note="pot. Shine-Dalgarno sequence"
CDS complement(5430..5474)
/note="peptide 1 (aa 1-15)"
/codon_start=5474
RBS complement(5482..5489)
/note="pot. Shine-Dalgarno sequence"
RBS 5780..5791
/note="pot. Shine-Dalgarno sequence"
CDS 5795..6454
/note="ORF"
/codon_start=5795
BASE COUNT 2406 a 903 c 1278 g 2104 t
ORIGIN
1 ttaaccatag tataataaat aatagtagtt aaagtagttt aagaggagat attaaaggga
61 tattttactt taaataaaaa ttaacaaggt ttataatttg tagtagttaa agtatttaaa
121 gaggtgggaa catgtgaagg ttcaaaggat agaagtggag aataagccgt atccattgta
181 tttattacta gataaagaat accagctaat agaaccagta atgaaattta ttaaatactt
241 agataatact ggtaagtctc ctaataccat taaggcatac tgctatcatt taaagttgct
301 gtacgagttc atggaacaga gaggtgttat tcttaatgat attaactttg agttgttagc
361 agacttcgta ggttggttga gatatccttc agcatcaaat gtaattgatc ttcagtcaaa
421 aaaagccata agagaagaaa cgacagtgaa tacaatttta aatgtagtta tgagttttct
481 tgattattta agtagattag gagaatttaa atcaattgat gtatttaaac aagcaaaggg
541 aagaaatttc aaaggatttt tacatcatgt taataagggt agataccaaa agaatgtctt
601 aaagttaagg gttaaaaaga aacagataag aacattgaga tcaaaggaag ttaagcaaat
661 tattgatgct tgtcatacga aaagagataa attaatttta atgcttatgt atgagggtgg
721 tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat attgtcactt gggacaatca
781 aatccattta acacctagag atgttaatgt taatgaagct tatattaaat taaggaagga
841 aagaacaata catgtgagta aagaacttat gtcactttat acagattact tgatatatga
901 gtatagtgag gaattggagc atgattatgt ttttatttcc ttaaaagaag gctattttgg
961 gaaaccacta aagtaccaaa gtgttcttga tctagttaga agaatagtta aaaggactgg
1021 aatagaattt acatcacata tgcttcgcca cactcacgca acgcagctaa ttagggaagg
1081 atgggatgtt gcgttcgttc aaaagagatt aggtcacgca catgttcaga caacgttaaa
1141 tacctatgtt catctttcag atcaggatat gaaaaatgag tttaataaat acctcgagag
1201 aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca gagtgagatt
1261 agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt tttgggctaa taatagttgg
1321 gatataagaa aatgtccaca tccttctgcc atagaattaa gtaagaatcc tgctttaagg
1381 aatcgttggg ttcgttttga acgtgttaaa aatctgtggt taagaacaga attgaaatat
1441 ttttattttt accatttaaa caatggaata tggaatgcaa aaactgtctg gattagaaaa
1501 ggaacagtaa ttaataaaat gttagatttc ttagatttaa agtatcctag cattacttca
1561 attactgaag ttcctattga aaaagcaatg acggagtata gaacttattt gacaaaacgg
1621 ggtgttagaa ttaccactac taattataag attactgcta atcaagaaaa aacacctgta
1681 aaagctaatt cctactatgt tactaatcta aaacaattta tggagtttta tgagaacttt
1741 tattttgatg gagaggagtg ggataaagac gtttgggata gacgtaactt acctttgcca
1801 gatgataagg ttaacccaac acaatatgaa tatacaatta actttaaagg gtttcggaat
1861 acatatttta aacaacttgt aaaaagatat tgtaagttga gattgaacgt ggatagcttt
1921 tcctatgtaa gtgatattgc ccaaagactt aaagagttct ttaattttct ggacatgaaa
1981 tttaaacaag ttcagagagt acaccaatta acgagagtgg aaattgaagc atatttaagt
2041 gaactaaaca tgatgggaat aaaacctagt acaataactg ggaggatctc tatattggaa
2101 ggactattta gtacccttct taggctagaa tgggatgatg ttccttccaa aatattaatt
2161 tattctgagg actatccgaa aataccaaga gcaaaaccac gctttataga tgaattcgtc
2221 ctagagcaat tgaacagtca tcttgataaa ttacccgaat atatagctac gatgactatg
2281 attgttcaag aatgtggaat gaggataagt gaattgtgca ccttgaaaaa aggctgtcta
2341 ttagaggaca aagatggaga tttcttttta aagtattatc aatggaaaat gaaaaaggag
2401 catatagttc caatatctaa agaggtagct ttacttatta aagttcggga agataaagtt
2461 tcagaggaat ttccagatag tgaatacctc tttccaagaa aagatggatc gccattaaaa
2521 caagaaacat ttagaggtga gttaaataaa ttagcttatg agcaaaatat agtggataaa
2581 tcaggtgaga tttatagatt ccatgcccat gcctttcgcc atacagtagg aacaagaatg
2641 attaacaacg ggatgcccca gcatattgtg cagaaatttt tggggcatga aagcccagaa
2701 atgacaagca gatacgctca tatctttgat gaaactctaa aaaatgaatt tactaaattt
2761 caggaaaaac tggttaccaa taatggagat gtgcttgatc tagatgaaga taatgaagtc
2821 gatgatgtag agcttcaatg gttcaagaaa aatataaatg cacaagtgct tccaaatggt
2881 tattgtagat tgccagtagt agcaggtggt tgtccacatg cgaatgcatg cttagattgc
2941 actcacttct gtaccagtaa gcaattctta ccacagcacg aagaacagtt agagcgtaca
3001 gaagagttat tagccatagc taaggataaa caatggcaaa gacaagtaga gactaatagc
3061 cgtgttaaag agcgtttaga acaaatcatt ggaagtttga cggggtaatt atcaatggat
3121 aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca
3181 agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt
3241 aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt
3301 aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc
3361 aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa
3421 ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat
3481 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
3541 gtcagcttta ttgaacagta atttaagtat atgtccaatc tagggtaagt aaattgagta
3601 tcaatataaa ctttatatga acataatcaa cgaggtgaaa tcatgagcaa tttgattaac
3661 ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt
3721 tcaatagttg gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc
3781 gatgtagatg ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta
3841 acagaaagac taatgactat atcaggaaag attggaaata cggattctgt tagaccactt
3901 gaagttacgg ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa
3961 tttatatacg gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc
4021 tatgatcctg atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt
4081 ggtcctgatt cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt
4141 aaggattctt tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta
4201 accctagctc gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct
4261 gcagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga
4321 aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca
4381 ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca
4441 ataacaggtg cttactttta aaactactga tttattgata aatattgaac aatttttggg
4501 aagaataaag cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac
4561 aatttgtaac tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg
4621 acatttgcat gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca
4681 cggtttaccc acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat
4741 ggttgatgtc gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat
4801 agtggtggta cttttttgag catttttata tccatctcca ccattaatag taaacccaaa
4861 gctcgttgca gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat
4921 ttagcctgac tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata
4981 ttaccatata tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga
5041 atcactttta tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca
5101 tcaatttcta tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct
5161 tttcctgatc cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt
5221 tcttttacat gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc
5281 attataacct tctccttact ggttataatg aactgacatt aagcgttcat tataaccaat
5341 taattttgat ttggttgata acgaacttta ttaataacaa atatagaaaa agtacccata
5401 cttttatctc ctttccattt ttttcattat gaatgagata aagtaatttc tactactgcg
5461 atactggtgc acataattga aacctcctta tgtaactgaa tatgattgta tcatcttttc
5521 attttagaat aaacctttat atgtaatttt gcaccttgac gacttcagat ggacgttttc
5581 ttcaaaataa gtatgttttg aaacttgata agagaagcca ctgtctttac taaaaagtaa
5641 aggctgcgat aaaggacagt gactttaata aagttgcact gttgtattgt ctttggataa
5701 aattgtctaa agggaagttt tatccccaac gaaaaaacag tctaaagtgg taagtaatga
5761 aagaatgtcg ataaagtagg aatgggggat ctttttgaat acaatagaat cacagtttga
5821 taaggttgca gaagattacg atttcgtgaa tgagcttttg aatgattatt cattctttgt
5881 gtctaatatg tctccaaaga aaggcagagc attagatatc ggatgtggct cgggtttgtt
5941 agtggagaaa ttagcaagtt attatgatga agtggtaggg attgatattt ctaatcaaat
6001 gctcgatctt gccaaatcta aacgtcaact aacaaatacg gtctatctga atatgaatgc
6061 agaacaactt aattttaatg agaagtttga ttttattgta agccgaacaa cctttcatca
6121 tttggatgat atagccagcg taatacagca aatgaaggaa ctgttgaatg aggaaggaag
6181 aatagttatt cttgataatg tatctgaagt cgagacaccg cctacctatg tttataaatt
6241 aggggcaatc caagagtttt taccgcattg tttcaaattt ggaataaaaa atgcgattag
6301 aatttataat cacaacacat ctaaatcatg gcttgagcat ttagcttcag ataagtacct
6361 atctgaacaa aattattacg atttatatga aaagttgttg cctggatgcc aattccataa
6421 aatgggttgg gcaatggggg ttgtctggac aaaataactt aaagctgtgg ctttgaaaag
6481 ttgaatttgt gattgttttc agagctatcg ggtgctttag tataaataac aatgaataaa
6541 aaatcagcct gtatcttatg ttactattaa gtaatttaag atacaggttt tactattttt
6601 gaaataagta ccttccactt caaatacatg tattcaaagt gaaagtattg atttaaatga
6661 ttcttttgta atttactctt gataagatgt a
//