GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOTN7GLM 574 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli left end of transposon Tn7 inserted into glmS terminator
ACCESSION X03474 M24254
KEYWORDS glmS gene; glutamine amidotransferase; terminator; transferase;
transposon; unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 574)
AUTHORS Gay,N.J., Tybulewicz,V.L. and Walker,J.E.
TITLE Insertion of transposon Tn7 into the Escherichia coli glmS
transcriptional terminator
JOURNAL Biochem. J. 234, 111-117 (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P13988; TNSA$ECOLI.
*source: strain=GD92::Tn7. see also V00620 Transposition of Tn7
into E. coli glmS transcriptional terminator abolishes
transcription termination at this site; the transcript now extends
into the left end of Tn7 and terminates at a new site "tm", 127
base pairs from the left end of Tn7. This region of the transposon
contains a long open reading frame which encodes a protein sequence
that is significantly related to the transposase proteins of the
transposable elements IS1 and Tn3.
From EMBL entry ECTN7GLM; dated 21-FEB-1991.
FEATURES Location/Qualifiers
CDS <1..9
/product="glutamine amidotransferase"
/gene="glmS"
/codon_start=1
misc_feature 17..37
/note="transcription terminator fragment (glmS)"
repeat_unit 17..28
/note="imp. inverted repeat A"
repeat_unit 33..37
/note="interrupted imp. inverted repeat A'"
transposon 38..>574
/note="inserted Tn7"
promoter 117..123
/note="put. -35 region (Tn7)"
promoter 140..145
/note="put. -10 region (Tn7)"
misc_feature 148..148
/note="put. transcription initiation site (Tn7)"
misc_signal 164..164
/note="put. new transcription termination site, tm site
(glmS)"
CDS 172..>574
/note="Tn7 URF1"
/codon_start=172
BASE COUNT 165 a 105 c 133 g 171 t
ORIGIN
1 acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
61 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
121 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
181 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
241 gggcatggta aagactatat tccatggcta acagtacaag aagttccttc ttcaggtcgt
301 tcccaccgta tttattctca taagacggga cgagtccatc atttgctatc tgacttagag
361 cttgctgttt ttctcagtct tgagtgggag agcagcgtgc tagatatacg cgagcagttc
421 cccttattac ctagtgatac caggcagatt gcaatagata gtggtattaa gcatcctgtt
481 attcgtggtg tagatcaggt tatgtctact gattttttag tggactgcaa agatggtcct
541 tttgagcagt ttgctattca agtcaaacct gcag
//GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTN7X 925 bp ds-DNA BCT 27-MAY-1991
DEFINITION E. coli left end of transposon TN7 inserted into glmS terminator
ACCESSION V00620
KEYWORDS glmS gene; glutamine amido transferase; transposon;
unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 925)
AUTHORS Lichtenstein,C. and Brenner,S.
TITLE Unique insertion site of Tn7 in the E. coli chromosome
JOURNAL Nature 297, 601-603 (1982)
STANDARD full automatic
REFERENCE 2 (bases 353 to 925)
AUTHORS Gay,N.J., Tybulewicz,V.L. and Walker,J.E.
TITLE Insertion of transposon Tn7 into the Escherichia coli glmS
transcriptional terminator
JOURNAL Biochem. J. 234, 111-117 (1986)
STANDARD full automatic
COMMENT From EMBL entry ISTN7X; dated 06-JUL-1989.
FEATURES Location/Qualifiers
CDS 362..364
/note="glmS translational stop codon"
/codon_start=362
misc_signal 369..389
/note="transcription terminator fragment"
transposon 390..>925
/note="Tn7 left end"
promoter 469..475
/note="put. -35 region (Tn7)"
promoter 492..497
/note="put. -10 region (Tn7)"
misc_feature 500..500
/note="put. transcription start site (Tn7)"
misc_signal 516..516
/note="put. new transcription termination site, tm site
(glmS)"
CDS 524..>925
/note="Tn7 URF1 (aa 1-134) (925 is 1st base in codon)"
/codon_start=524
BASE COUNT 248 a 194 c 227 g 256 t
ORIGIN
1 gatctcttac attcacgctg aagcctacgc tgctggcgaa ctgaaacacg gtccgctggc
61 gctaattgat gccgatatgc cggttattgt tgttgcaccg aacaacgaat tgctggaaaa
121 actgaaatcc aacattgaag aagttcgcgc gcgtggcggt cagttgtatg tcttcgccga
181 tcaggatgcg ggttttgtaa gtagcgataa catgcacatc atcgagatgc cgcatgtgga
241 agaggtgatt gcaccgatct tctacaccgt tccgctgcag ctgctggctt accatgtcgc
301 gctgatcaaa ggcaccgacg ttgaccagcc gcgtaacctg gcaaaatcgg ttacggttga
361 gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac
421 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa
481 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
541 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg
601 taaagactat attccatggc taacagtaca gaagttcctt cttcaggtcg ttcccaccgt
661 atttattctc ataagacggg acgagtccat catttgctat ctgacttaga gcttgctgtt
721 tttctcagtc ttgagtggga gagcagcgtg ctagatatac gcgagcagtt ccccttatta
781 cctagtgata ccaggcagat tgcaatagat agtggtatta agcatcctgt tattcgtggt
841 gtagatcagg ttatgtctac tgatttttta gtggactgca aagatggtcc ttttgagcag
901 tttgctattc aagtcaaacc tgcag
//