GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOTN7GLM 574 bp ds-DNA BCT 18-MAY-1991 DEFINITION E. coli left end of transposon Tn7 inserted into glmS terminator ACCESSION X03474 M24254 KEYWORDS glmS gene; glutamine amidotransferase; terminator; transferase; transposon; unidentified reading frame. SOURCE Escherichia coli DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 574) AUTHORS Gay,N.J., Tybulewicz,V.L. and Walker,J.E. TITLE Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator JOURNAL Biochem. J. 234, 111-117 (1986) STANDARD full automatic COMMENT SWISS-PROT; P13988; TNSA$ECOLI. *source: strain=GD92::Tn7. see also V00620 Transposition of Tn7 into E. coli glmS transcriptional terminator abolishes transcription termination at this site; the transcript now extends into the left end of Tn7 and terminates at a new site "tm", 127 base pairs from the left end of Tn7. This region of the transposon contains a long open reading frame which encodes a protein sequence that is significantly related to the transposase proteins of the transposable elements IS1 and Tn3. From EMBL entry ECTN7GLM; dated 21-FEB-1991. FEATURES Location/Qualifiers CDS <1..9 /product="glutamine amidotransferase" /gene="glmS" /codon_start=1 misc_feature 17..37 /note="transcription terminator fragment (glmS)" repeat_unit 17..28 /note="imp. inverted repeat A" repeat_unit 33..37 /note="interrupted imp. inverted repeat A'" transposon 38..>574 /note="inserted Tn7" promoter 117..123 /note="put. -35 region (Tn7)" promoter 140..145 /note="put. -10 region (Tn7)" misc_feature 148..148 /note="put. transcription initiation site (Tn7)" misc_signal 164..164 /note="put. new transcription termination site, tm site (glmS)" CDS 172..>574 /note="Tn7 URF1" /codon_start=172 BASE COUNT 165 a 105 c 133 g 171 t ORIGIN 1 acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta 61 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt 121 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa 181 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa 241 gggcatggta aagactatat tccatggcta acagtacaag aagttccttc ttcaggtcgt 301 tcccaccgta tttattctca taagacggga cgagtccatc atttgctatc tgacttagag 361 cttgctgttt ttctcagtct tgagtgggag agcagcgtgc tagatatacg cgagcagttc 421 cccttattac ctagtgatac caggcagatt gcaatagata gtggtattaa gcatcctgtt 481 attcgtggtg tagatcaggt tatgtctact gattttttag tggactgcaa agatggtcct 541 tttgagcagt ttgctattca agtcaaacct gcag //
GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTN7X 925 bp ds-DNA BCT 27-MAY-1991 DEFINITION E. coli left end of transposon TN7 inserted into glmS terminator ACCESSION V00620 KEYWORDS glmS gene; glutamine amido transferase; transposon; unidentified reading frame. SOURCE Escherichia coli DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 925) AUTHORS Lichtenstein,C. and Brenner,S. TITLE Unique insertion site of Tn7 in the E. coli chromosome JOURNAL Nature 297, 601-603 (1982) STANDARD full automatic REFERENCE 2 (bases 353 to 925) AUTHORS Gay,N.J., Tybulewicz,V.L. and Walker,J.E. TITLE Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator JOURNAL Biochem. J. 234, 111-117 (1986) STANDARD full automatic COMMENT From EMBL entry ISTN7X; dated 06-JUL-1989. FEATURES Location/Qualifiers CDS 362..364 /note="glmS translational stop codon" /codon_start=362 misc_signal 369..389 /note="transcription terminator fragment" transposon 390..>925 /note="Tn7 left end" promoter 469..475 /note="put. -35 region (Tn7)" promoter 492..497 /note="put. -10 region (Tn7)" misc_feature 500..500 /note="put. transcription start site (Tn7)" misc_signal 516..516 /note="put. new transcription termination site, tm site (glmS)" CDS 524..>925 /note="Tn7 URF1 (aa 1-134) (925 is 1st base in codon)" /codon_start=524 BASE COUNT 248 a 194 c 227 g 256 t ORIGIN 1 gatctcttac attcacgctg aagcctacgc tgctggcgaa ctgaaacacg gtccgctggc 61 gctaattgat gccgatatgc cggttattgt tgttgcaccg aacaacgaat tgctggaaaa 121 actgaaatcc aacattgaag aagttcgcgc gcgtggcggt cagttgtatg tcttcgccga 181 tcaggatgcg ggttttgtaa gtagcgataa catgcacatc atcgagatgc cgcatgtgga 241 agaggtgatt gcaccgatct tctacaccgt tccgctgcag ctgctggctt accatgtcgc 301 gctgatcaaa ggcaccgacg ttgaccagcc gcgtaacctg gcaaaatcgg ttacggttga 361 gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac 421 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa 481 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc 541 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg 601 taaagactat attccatggc taacagtaca gaagttcctt cttcaggtcg ttcccaccgt 661 atttattctc ataagacggg acgagtccat catttgctat ctgacttaga gcttgctgtt 721 tttctcagtc ttgagtggga gagcagcgtg ctagatatac gcgagcagtt ccccttatta 781 cctagtgata ccaggcagat tgcaatagat agtggtatta agcatcctgt tattcgtggt 841 gtagatcagg ttatgtctac tgatttttta gtggactgca aagatggtcc ttttgagcag 901 tttgctattc aagtcaaacc tgcag //